Distinct Radiobiological Responses to BNCT in SAS Oral Squamous Cell Carcinoma and MCF-7 Breast Cancer Cells
Abstract
This work compared the radiobiological responses of SAS oral squamous cell carcinoma cells and MCF-7 breast cancer cells following accelerator-based boron neutron capture therapy (BNCT). Neutrons were generated by bombarding a lithium target with proton beams, followed by moderation to obtain sufficient thermal neutrons for BNCT irradiation. Boronophenylalanine (BPA) was used as the boron delivery agent. BNCT-induced biological responses were evaluated by gamma-H2AX immunofluorescence staining, cell-cycle analysis, apoptosis analysis, and clonogenic survival assays. BNCT induced marked gamma-H2AX foci formation in both cell lines, indicating DNA damage-associated responses after irradiation. The two cell lines further showed distinct post-irradiation outcomes. SAS cells exhibited stronger clonogenic suppression and prominent G2/M accumulation, whereas MCF-7 cells showed sustained G0/G1 accumulation and delayed apoptosis. These results suggest that BNCT sensitivity is determined by both boron accumulation and cell-line-specific biological characteristics. This work provides experimental evidence highlighting the importance of tumor-dependent cellular responses in understanding and optimizing BNCT efficacy.
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BibTeX
@misc{zhao2026distinct,
title = {{Distinct Radiobiological Responses to BNCT in SAS Oral Squamous Cell Carcinoma and MCF-7 Breast Cancer Cells}},
author = {Yuxiang Zhao and Zhao Sun and Changming Wang and Jianghao Lai and Jie Zhou and Zhencen He and Zhimin Hu},
howpublished = {arXiv},
year = {2026},
month = sep,
doi = {10.48550/arxiv.2609.05931},
eprint = {2609.05931},
archivePrefix = {arXiv},
url = {https://arxiv.org/abs/2609.05931},
}
RIS
TY - UNPB
TI - Distinct Radiobiological Responses to BNCT in SAS Oral Squamous Cell Carcinoma and MCF-7 Breast Cancer Cells
AU - Yuxiang Zhao
AU - Zhao Sun
AU - Changming Wang
AU - Jianghao Lai
AU - Jie Zhou
AU - Zhencen He
AU - Zhimin Hu
PY - 2026
DA - 2026/09/05
PB - arXiv
DO - 10.48550/arxiv.2609.05931
UR - https://arxiv.org/abs/2609.05931
AB - This work compared the radiobiological responses of SAS oral squamous cell carcinoma cells and MCF-7 breast cancer cells following accelerator-based boron neutron capture therapy (BNCT). Neutrons were generated by bombarding a lithium target with proton beams, followed by moderation to obtain sufficient thermal neutrons for BNCT irradiation. Boronophenylalanine (BPA) was used as the boron delivery agent. BNCT-induced biological responses were evaluated by gamma-H2AX immunofluorescence staining, cell-cycle analysis, apoptosis analysis, and clonogenic survival assays. BNCT induced marked gamma-H2AX foci formation in both cell lines, indicating DNA damage-associated responses after irradiation. The two cell lines further showed distinct post-irradiation outcomes. SAS cells exhibited stronger clonogenic suppression and prominent G2/M accumulation, whereas MCF-7 cells showed sustained G0/G1 accumulation and delayed apoptosis. These results suggest that BNCT sensitivity is determined by both boron accumulation and cell-line-specific biological characteristics. This work provides experimental evidence highlighting the importance of tumor-dependent cellular responses in understanding and optimizing BNCT efficacy.
ER -