Genetic determinants that permit growth without the core septin Cdc12 in Cryptococcus neoformans
Abstract
Septins are conserved cytoskeletal GTPases that regulate morphogenesis, cytokinesis, and cell-surface organization in fungi. In most model and pathogenic yeasts that proliferate by budding, including Saccharomyces cerevisiae and Candida albicans, core septin subunits such as Cdc3 and Cdc12 are essential and their loss results in lethal cytokinetic defects. In Cryptococcus neoformans, by contrast, deletion of CDC12 or CDC3 yields viable cells that grow normally under permissive conditions, raising the question of how this pathogen maintains cell integrity without a functional septin complex. To address this, we compared the transcriptomes of wild-type cells and a cdc12Δ mutant. The cdc12Δ strain showed a focused set of transcriptional changes, with induction of genes involved in cell wall remodeling, stress-dependent morphogenetic programs, and pathways that promote fitness under compromised cytoskeletal conditions. Genetic analysis of the most highly induced genes uncovered factors that contribute to proliferation in the absence of Cdc12, including a previously uncharacterized aspartic-type endopeptidase that exhibits a genetic interaction with the loss of septin function. These findings outline the compensatory circuitry that permits septin-independent growth in C. neoformans and help explain why this pathogen can tolerate the absence of the septin complex. This intrinsic plasticity highlights C. neoformans as a powerful comparative model for studying the evolution and function of septins.
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BibTeX
@article{stempinski2026genetic,
title = {{Genetic determinants that permit growth without the core septin Cdc12 in Cryptococcus neoformans}},
author = {Piotr R Stempinski and Hannah S Akahoho and Rooksana E Noorai and Vijay Shankar and Lukasz Kozubowski},
journal = {G3: Genes | Genomes | Genetics},
year = {2026},
month = aug,
doi = {10.1093/g3journal/jkag240},
url = {https://doi.org/10.1093/g3journal/jkag240},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Genetic determinants that permit growth without the core septin Cdc12 in Cryptococcus neoformans
AU - Piotr R Stempinski
AU - Hannah S Akahoho
AU - Rooksana E Noorai
AU - Vijay Shankar
AU - Lukasz Kozubowski
PY - 2026
DA - 2026/08/29
JO - G3: Genes | Genomes | Genetics
DO - 10.1093/g3journal/jkag240
UR - https://doi.org/10.1093/g3journal/jkag240
AB - Septins are conserved cytoskeletal GTPases that regulate morphogenesis, cytokinesis, and cell-surface organization in fungi. In most model and pathogenic yeasts that proliferate by budding, including Saccharomyces cerevisiae and Candida albicans, core septin subunits such as Cdc3 and Cdc12 are essential and their loss results in lethal cytokinetic defects. In Cryptococcus neoformans, by contrast, deletion of CDC12 or CDC3 yields viable cells that grow normally under permissive conditions, raising the question of how this pathogen maintains cell integrity without a functional septin complex. To address this, we compared the transcriptomes of wild-type cells and a cdc12Δ mutant. The cdc12Δ strain showed a focused set of transcriptional changes, with induction of genes involved in cell wall remodeling, stress-dependent morphogenetic programs, and pathways that promote fitness under compromised cytoskeletal conditions. Genetic analysis of the most highly induced genes uncovered factors that contribute to proliferation in the absence of Cdc12, including a previously uncharacterized aspartic-type endopeptidase that exhibits a genetic interaction with the loss of septin function. These findings outline the compensatory circuitry that permits septin-independent growth in C. neoformans and help explain why this pathogen can tolerate the absence of the septin complex. This intrinsic plasticity highlights C. neoformans as a powerful comparative model for studying the evolution and function of septins.
SN - 2160-1836
ER -