Investigation of ferroptosis-related gene expression and oxidative stress markers in end-stage diabetic kidney disease: A case–control analysis
- 1Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
- 2Department of Nephrology, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
End-stage renal disease (ESRD), the most advanced stage of chronic kidney disease, is a major diabetes-related complication associated with severe metabolic and oxidative disturbances. Ferroptosis, an iron-dependent form of regulated cell death, may contribute to diabetic kidney disease (DKD) progression; however, its role in ESRD remains unclear. This case-control study investigated oxidative stress markers and ferroptosis-related gene expression in ESRD patients secondary to DKD and healthy controls. Biochemical parameters, oxidative stress markers, and the mRNA expression of tumor suppressor genes (p21 and p53) and nuclear factor erythroid 2-related factor 2 (Nrf2) were assessed in peripheral blood mononuclear cells. Group comparisons were performed using appropriate statistical tests, and logistic regression was used to evaluate the relationship between ESRD status. ESRD patients showed significantly higher Nrf2 expression (P < 0.0001) and lower p53 expression (P = 0.026), while p21 expression did not differ significantly (P = 0.32). Catalase activity and malondialdehyde levels were significantly increased in ESRD (P = 0.0001), whereas glutathione peroxidase activity was decreased (P = 0.018). Logistic regression demonstrated significant associations between ESRD and CAT activity (OR = 1.082), MDA levels (OR = 3.627), GPx activity (OR = 0.985), Nrf2 expression (OR = 2.133), and p53 expression (OR = 0.711) (all P < 0.05). These findings suggest that ESRD secondary to DKD is associated with significant alterations in oxidative stress status and changes in the expression of genes linked to ferroptosis-related pathways, providing indirect evidence of their possible involvement
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BibTeX
@article{ebrh2026investigation,
title = {{Investigation of ferroptosis-related gene expression and oxidative stress markers in end-stage diabetic kidney disease: A case–control analysis}},
author = {Shaker Ghalioun Ebrh and Gholamreza Dehghan and Hamid Tayebi Khosroshahi and Mohammad Khalaj Kondori},
journal = {International Journal of Molecular and Cellular Medicine},
year = {2026},
month = jun,
doi = {10.22088/ijmcm.bums.15.2.1356},
url = {https://doi.org/10.22088/ijmcm.bums.15.2.1356},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Investigation of ferroptosis-related gene expression and oxidative stress markers in end-stage diabetic kidney disease: A case–control analysis
AU - Shaker Ghalioun Ebrh
AU - Gholamreza Dehghan
AU - Hamid Tayebi Khosroshahi
AU - Mohammad Khalaj Kondori
PY - 2026
DA - 2026/06/01
JO - International Journal of Molecular and Cellular Medicine
DO - 10.22088/ijmcm.bums.15.2.1356
UR - https://doi.org/10.22088/ijmcm.bums.15.2.1356
AB - End-stage renal disease (ESRD), the most advanced stage of chronic kidney disease, is a major diabetes-related complication associated with severe metabolic and oxidative disturbances. Ferroptosis, an iron-dependent form of regulated cell death, may contribute to diabetic kidney disease (DKD) progression; however, its role in ESRD remains unclear. This case-control study investigated oxidative stress markers and ferroptosis-related gene expression in ESRD patients secondary to DKD and healthy controls. Biochemical parameters, oxidative stress markers, and the mRNA expression of tumor suppressor genes (p21 and p53) and nuclear factor erythroid 2-related factor 2 (Nrf2) were assessed in peripheral blood mononuclear cells. Group comparisons were performed using appropriate statistical tests, and logistic regression was used to evaluate the relationship between ESRD status. ESRD patients showed significantly higher Nrf2 expression (P < 0.0001) and lower p53 expression (P = 0.026), while p21 expression did not differ significantly (P = 0.32). Catalase activity and malondialdehyde levels were significantly increased in ESRD (P = 0.0001), whereas glutathione peroxidase activity was decreased (P = 0.018). Logistic regression demonstrated significant associations between ESRD and CAT activity (OR = 1.082), MDA levels (OR = 3.627), GPx activity (OR = 0.985), Nrf2 expression (OR = 2.133), and p53 expression (OR = 0.711) (all P < 0.05). These findings suggest that ESRD secondary to DKD is associated with significant alterations in oxidative stress status and changes in the expression of genes linked to ferroptosis-related pathways, providing indirect evidence of their possible involvement
SN - 2251-9637
SN - 2251-9645
ER -