Novel method for extraction and quantitation of 6PPD-Q in solid matrices using ultrasonic-assisted extraction (UAE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
- 1City of Tacoma Environmental Services Laboratory, 326 E D St., Tacoma, WA, 98421, United States.
Abstract
This study presents a UAE-LC-MS/MS method for the extraction and quantitation of 6PPD-Q in stormwater sediment matrices. The final method uses ultrasonic-assisted extraction with a hexane:ethyl acetate solvent system and LC-MS/MS instrumental analysis with matrix-matched calibration, and demonstrated acceptable sensitivity, selectivity, and reproducibility. This study addressed challenges specific to sediment matrix, a lack of commercially available reference material, and co-extracted chromatographic interferences. Matrix effects were reduced through optimized extraction parameters, rather than post-extraction cleanup procedures. A total of 28 sediment samples were analyzed using LC-MS/MS to evaluate 6PPD-Q concentrations, resulting in values ranging from 11.4 ng/g to 691 ng/g, with a mean value of 171 ng/g. The method achieved a method detection limit of 1.1 ng/g and limit of quantitation of 5 ng/g using a 250 mg sample size. The mean recovery rate of 6PPD-Q in laboratory control samples was 80.1% (range: 74.8% - 82.3%).•Extract sediment sample with hexane:ethyl acetate mix and dry.•Reconstitute with acetonitrile.•Analyze using LC-MS/MS.
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BibTeX
@article{chontofalsky2026novel,
title = {{Novel method for extraction and quantitation of 6PPD-Q in solid matrices using ultrasonic-assisted extraction (UAE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).}},
author = {Thomas Chontofalsky and Fatima Alsinai and Terri Torres and Monica Herbert and Tiffany Ryan},
journal = {MethodsX},
year = {2026},
month = sep,
doi = {10.1016/j.mex.2026.104178},
url = {https://europepmc.org/article/MED/42898596},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Novel method for extraction and quantitation of 6PPD-Q in solid matrices using ultrasonic-assisted extraction (UAE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
AU - Thomas Chontofalsky
AU - Fatima Alsinai
AU - Terri Torres
AU - Monica Herbert
AU - Tiffany Ryan
PY - 2026
DA - 2026/09/29
JO - MethodsX
DO - 10.1016/j.mex.2026.104178
UR - https://europepmc.org/article/MED/42898596
AB - This study presents a UAE-LC-MS/MS method for the extraction and quantitation of 6PPD-Q in stormwater sediment matrices. The final method uses ultrasonic-assisted extraction with a hexane:ethyl acetate solvent system and LC-MS/MS instrumental analysis with matrix-matched calibration, and demonstrated acceptable sensitivity, selectivity, and reproducibility. This study addressed challenges specific to sediment matrix, a lack of commercially available reference material, and co-extracted chromatographic interferences. Matrix effects were reduced through optimized extraction parameters, rather than post-extraction cleanup procedures. A total of 28 sediment samples were analyzed using LC-MS/MS to evaluate 6PPD-Q concentrations, resulting in values ranging from 11.4 ng/g to 691 ng/g, with a mean value of 171 ng/g. The method achieved a method detection limit of 1.1 ng/g and limit of quantitation of 5 ng/g using a 250 mg sample size. The mean recovery rate of 6PPD-Q in laboratory control samples was 80.1% (range: 74.8% - 82.3%).•Extract sediment sample with hexane:ethyl acetate mix and dry.•Reconstitute with acetonitrile.•Analyze using LC-MS/MS.
SN - 2215-0161
ER -