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Peer-reviewedCC BYStructure

Streamlined montage cryo-electron tomography for exploring the ultrastructure of cells and tissues

  • Ryan K. Hylton1,2
  • Micaela Boiero Sanders1,2
  • Adriana Georgiana Prajica1,2
  • Gavin Rice1,2
  • Stefan Raunser3,2
  1. 1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
  2. 2Max Planck Institute of Molecular Physiology
  3. 3Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany. Electronic address: [email protected]
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Abstract

Cryo-electron tomography reveals the architecture of biological specimens at molecular resolution, albeit with a limited field of view. Montage tomography overcomes this limitation, but existing strategies depend on complex dose distribution imaging schemes or hardware modifications which prevent widespread adoption of the method. To lower the barrier to entry, we developed montaging via streamlined acquisition and integration of cryotomograms (MOSAIC), a straightforward workflow that deviates minimally from conventional image acquisition and leverages our software MontageMaker to merge overlapping tilt series. The resulting montage tomograms are produced with high throughput, retain nanoscale detail despite the uneven accumulation of dose, and are easily acquired on a wide variety of biological samples. Most importantly, this approach can be implemented immediately by any lab with access to automated cryo-ET data collection.

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Cite

BibTeXRIS
BibTeX
@article{hylton2026streamlined,
  title = {{Streamlined montage cryo-electron tomography for exploring the ultrastructure of cells and tissues}},
  author = {Ryan K. Hylton and Micaela Boiero Sanders and Adriana Georgiana Prajica and Gavin Rice and Stefan Raunser},
  journal = {Structure},
  year = {2026},
  month = jul,
  doi = {10.1016/j.str.2026.07.001},
  url = {https://doi.org/10.1016/j.str.2026.07.001},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Streamlined montage cryo-electron tomography for exploring the ultrastructure of cells and tissues
AU  - Ryan K. Hylton
AU  - Micaela Boiero Sanders
AU  - Adriana Georgiana Prajica
AU  - Gavin Rice
AU  - Stefan Raunser
PY  - 2026
DA  - 2026/07/27
JO  - Structure
DO  - 10.1016/j.str.2026.07.001
UR  - https://doi.org/10.1016/j.str.2026.07.001
AB  - Cryo-electron tomography reveals the architecture of biological specimens at molecular resolution, albeit with a limited field of view. Montage tomography overcomes this limitation, but existing strategies depend on complex dose distribution imaging schemes or hardware modifications which prevent widespread adoption of the method. To lower the barrier to entry, we developed montaging via streamlined acquisition and integration of cryotomograms (MOSAIC), a straightforward workflow that deviates minimally from conventional image acquisition and leverages our software MontageMaker to merge overlapping tilt series. The resulting montage tomograms are produced with high throughput, retain nanoscale detail despite the uneven accumulation of dose, and are easily acquired on a wide variety of biological samples. Most importantly, this approach can be implemented immediately by any lab with access to automated cryo-ET data collection.
SN  - 0969-2126
SN  - 1878-4186
ER  -