The ultrastructure of the mature wheat grain after resin embedding, as observed using atomic force microscopy

Abstract

This study examines the ultrastructure of the outer layers of hexaploid wheat (Triticum aestivum L.) seeds using atomic force microscopy (AFM) in air tapping mode. The specimens were resin-embedded after hydration. A standardised protocol for preparing specimens specifically for AFM investigations is presented, focusing on revealing the ultrastructure while minimising artefacts and optimising the resolution of the structural morphology. AFM provides a comprehensive histological description of the hydrated mature wheat seed, encompassing each layer from the outer pericarp to the starchy endosperm. This study highlights the ultrastructural details of the tissues in their hydrated state, particularly with regard to morphology and size. Thus, AFM shows great potential for revealing intricate details of plant tissues structure and ultrastructure.

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BibTeX RIS

BibTeX

@article{gaillard2026ultrastructure,
  title = {{The ultrastructure of the mature wheat grain after resin embedding, as observed using atomic force microscopy}},
  author = {Cedric Gaillard},
  journal = {Journal of Cereal Science},
  year = {2026},
  month = oct,
  doi = {10.1016/j.jcs.2026.104377},
  url = {https://hal.inrae.fr/hal-05797371v1},
  note = {CC BY 4.0},
}

RIS

TY  - JOUR
TI  - The ultrastructure of the mature wheat grain after resin embedding, as observed using atomic force microscopy
AU  - Cedric Gaillard
PY  - 2026
DA  - 2026/10/13
JO  - Journal of Cereal Science
DO  - 10.1016/j.jcs.2026.104377
UR  - https://hal.inrae.fr/hal-05797371v1
AB  - This study examines the ultrastructure of the outer layers of hexaploid wheat (Triticum aestivum L.) seeds using atomic force microscopy (AFM) in air tapping mode. The specimens were resin-embedded after hydration. A standardised protocol for preparing specimens specifically for AFM investigations is presented, focusing on revealing the ultrastructure while minimising artefacts and optimising the resolution of the structural morphology. AFM provides a comprehensive histological description of the hydrated mature wheat seed, encompassing each layer from the outer pericarp to the starchy endosperm. This study highlights the ultrastructural details of the tissues in their hydrated state, particularly with regard to morphology and size. Thus, AFM shows great potential for revealing intricate details of plant tissues structure and ultrastructure.
SN  - 0733-5210
SN  - 1095-9963
ER  -

Abstract

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