Cryo-EM Structures of Infectious Low-Twist α-Synuclein Filaments in Lewy Bodies
- 1University of California San Francisco
Abstract
-Synuclein (aSyn) aggregation within Lewy bodies underlies Parkinson's disease and associated dementias. These aggregates are often comprised of amyloid filaments, most of which appear untwisted or have extremely low twist, complicating high-resolution structural determination by cryogenic electron microscopy (cryo-EM). Here, we isolated filaments from the brain tissue of a patient with dementia with Lewy bodies and show infectivity in a cell line system that propagates Lewy aSyn. Then, we determined 2.3-2.9 [A] cryo-EM structures of low-twist aSyn filaments from this tissue and from a publicly available Parkinson's disease dementia dataset. In both structures, the protofilament adopts a fold highly similar to the canonical Lewy fold with conserved cofactor and peptidic densities. Compared to previously resolved high-twist filaments, the low-twist filament is left-handed and flattened along the filament axis, resulting in register shifts of interlayer contacts, while preserving the overall architecture. Our results establish that low-twist filaments, which include over 75% of disease-associated Lewy aSyn amyloids, share a common structural core with high-twist forms and are the predominant aSyn species in samples that engage in templated infection.
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BibTeX
@misc{yan2026cryoem,
title = {{Cryo-EM Structures of Infectious Low-Twist α-Synuclein Filaments in Lewy Bodies}},
author = {N. L. Yan and M. Mayer and A. A. Melo and J. I. Ayers and D. A. Mordes and W. W. Seeley and S. B. Prusiner and E. Tse and V. G. Chittoor-Vinod and G. E. Merz},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.16.752119},
url = {https://www.biorxiv.org/content/10.64898/2026.09.16.752119v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Cryo-EM Structures of Infectious Low-Twist α-Synuclein Filaments in Lewy Bodies
AU - N. L. Yan
AU - M. Mayer
AU - A. A. Melo
AU - J. I. Ayers
AU - D. A. Mordes
AU - W. W. Seeley
AU - S. B. Prusiner
AU - E. Tse
AU - V. G. Chittoor-Vinod
AU - G. E. Merz
PY - 2026
DA - 2026/09/16
PB - bioRxiv
DO - 10.64898/2026.09.16.752119
UR - https://www.biorxiv.org/content/10.64898/2026.09.16.752119v1
AB - -Synuclein (aSyn) aggregation within Lewy bodies underlies Parkinson's disease and associated dementias. These aggregates are often comprised of amyloid filaments, most of which appear untwisted or have extremely low twist, complicating high-resolution structural determination by cryogenic electron microscopy (cryo-EM). Here, we isolated filaments from the brain tissue of a patient with dementia with Lewy bodies and show infectivity in a cell line system that propagates Lewy aSyn. Then, we determined 2.3-2.9 [A] cryo-EM structures of low-twist aSyn filaments from this tissue and from a publicly available Parkinson's disease dementia dataset. In both structures, the protofilament adopts a fold highly similar to the canonical Lewy fold with conserved cofactor and peptidic densities. Compared to previously resolved high-twist filaments, the low-twist filament is left-handed and flattened along the filament axis, resulting in register shifts of interlayer contacts, while preserving the overall architecture. Our results establish that low-twist filaments, which include over 75% of disease-associated Lewy aSyn amyloids, share a common structural core with high-twist forms and are the predominant aSyn species in samples that engage in templated infection.
ER -