Genomic analysis of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from a dog with a severe episode of self-trauma and urinary tract infection
- 1Department of Veterinary Medicine, University Vila Velha (UVV), Av. Comissário José Dantas de Melo, n.21, Vila Velha, ES 29102-920 Brazil
- 2Department of Animal Clinic, Surgery and Reproduction, School of Veterinary Medicine, São Paulo State University (UNESP), 793 Clóvis Pestana St, Araçatuba, São Paulo 16050-680 Brazil
- 3Department of Pharmaceutical Sciences, Health Sciences Center, Federal University of Paraíba, João Pessoa, Paraíba Brazil
Abstract
Extended-spectrum β-lactamase (ESBL)-producing Enterobacterales are emerging pathogens of major importance in veterinary medicine. This study describes the clinical, microbiological, and genomic characteristics of ESBL-producing Escherichia coli and Klebsiella pneumoniae isolated from a dog with paraparesis and concurrent pyelonephritis, chronic cystitis, and a self-traumatized limb lesion. A 12-year-old mixed-breed female dog underwent repeated antimicrobial therapy during hospitalization. Urine obtained by cystocentesis and wound samples were cultured, and ESBL-producing strains were characterized by antimicrobial susceptibility testing and whole-genome sequence-based analysis. CTX-M-55-producing E. coli sequence type (ST) 457, phylogroup F, O11:H25, and fimH145, was recovered from urine and wound samples, while the wound sample also yielded a CTX-M-15- and SHV-2-producing K. pneumoniae ST39, KL149:OL2α.2. The two closely related E. coli strains differed by 96 single nucleotide polymorphisms, despite sharing the same ST, resistome, plasmid replicons, and virulence profile. ESBL-positive strains carried multiple virulence genes, with the E. coli strains classified as extraintestinal pathogenic E. coli strains. To the best of our knowledge, this is the first report of ESBL-producing K. pneumoniae ST39 in an animal in Brazil. The co-occurrence of ESBL-producing E. coli ST457 and K. pneumoniae ST39 in the same dog highlights the potential for companion animals to harbor clinically relevant multidrug-resistant lineages and reinforces the need for continuous antimicrobial resistance monitoring and antimicrobial stewardship in this sector.
Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s11259-026-11615-4.
Related
- Phenotypic and molecular characterization of extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae isolates from communities in South Western Uganda
- Genomic analysis and antimicrobial resistance profiling of Klebsiella pneumoniae clinical isolates from urinary tract infections in India: prevalence of class A β-lactamase with molecular docking and simulation insights.
- Assessment on the Effects of Glycerol Monolaurate on the Antibacterial and Beta-Lactamase blaTEM and blaSHV Gene Expression in Multidrug-Resistant Klebsiella pneumoniae.
- Rapid inter-regional and cross-border dissemination of carbapenemase-producing Klebsiella pneumoniae ST6668, European Union, April 2022 to August 2026.
- From sepsis to necrotizing enterocolitis with appendiceal perforation: a case report of successful salvage therapy with aztreonam–avibactam in an extremely preterm infant with NDM-producing Klebsiella pneumoniae
BibTeX
@article{repossi2026genomic,
title = {{Genomic analysis of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from a dog with a severe episode of self-trauma and urinary tract infection}},
author = {Camile Pereira Repossi and Sara Colodetti Paiva and Matheus Nunes Simões and Bruna Modenese Fernandes and João Vitor Oliveira Silva and Thiago Aragão de Araújo and Carolina Magri Ferraz and Igor Luiz Salardani Senhorello and Gabriel Augusto Marques Rossi and João Pedro Rueda Furlan},
journal = {Veterinary Research Communications},
year = {2026},
month = nov,
doi = {10.1007/s11259-026-11615-4},
url = {https://doi.org/10.1007/s11259-026-11615-4},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Genomic analysis of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from a dog with a severe episode of self-trauma and urinary tract infection
AU - Camile Pereira Repossi
AU - Sara Colodetti Paiva
AU - Matheus Nunes Simões
AU - Bruna Modenese Fernandes
AU - João Vitor Oliveira Silva
AU - Thiago Aragão de Araújo
AU - Carolina Magri Ferraz
AU - Igor Luiz Salardani Senhorello
AU - Gabriel Augusto Marques Rossi
AU - João Pedro Rueda Furlan
PY - 2026
DA - 2026/11/06
JO - Veterinary Research Communications
DO - 10.1007/s11259-026-11615-4
UR - https://doi.org/10.1007/s11259-026-11615-4
AB - Extended-spectrum β-lactamase (ESBL)-producing Enterobacterales are emerging pathogens of major importance in veterinary medicine. This study describes the clinical, microbiological, and genomic characteristics of ESBL-producing Escherichia coli and Klebsiella pneumoniae isolated from a dog with paraparesis and concurrent pyelonephritis, chronic cystitis, and a self-traumatized limb lesion. A 12-year-old mixed-breed female dog underwent repeated antimicrobial therapy during hospitalization. Urine obtained by cystocentesis and wound samples were cultured, and ESBL-producing strains were characterized by antimicrobial susceptibility testing and whole-genome sequence-based analysis. CTX-M-55-producing E. coli sequence type (ST) 457, phylogroup F, O11:H25, and fimH145, was recovered from urine and wound samples, while the wound sample also yielded a CTX-M-15- and SHV-2-producing K. pneumoniae ST39, KL149:OL2α.2. The two closely related E. coli strains differed by 96 single nucleotide polymorphisms, despite sharing the same ST, resistome, plasmid replicons, and virulence profile. ESBL-positive strains carried multiple virulence genes, with the E. coli strains classified as extraintestinal pathogenic E. coli strains. To the best of our knowledge, this is the first report of ESBL-producing K. pneumoniae ST39 in an animal in Brazil. The co-occurrence of ESBL-producing E. coli ST457 and K. pneumoniae ST39 in the same dog highlights the potential for companion animals to harbor clinically relevant multidrug-resistant lineages and reinforces the need for continuous antimicrobial resistance monitoring and antimicrobial stewardship in this sector. Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s11259-026-11615-4.
SN - 0165-7380
SN - 1573-7446
ER -