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Peer-reviewedCC BYAnimals : an open access journal from MDPI

MAPK Signaling Contributes to Primary Follicle Development in Zebrafish (Danio rerio).

  • Guangjing Zhang1,2
  • Jing Huang1
  • Xiudan Yuan1,3
  • Yunpeng Fan1,4
  • Fangyuan Peng1,5
  • Wen Fu1
  • Jinhui Liu1
  • Wenbin Liu1
  • Liangyue Peng1
  • Yamei Xiao1
5 affiliations
  1. 1College of Life Sciences, Hunan Normal University, Changsha 410081, China.
  2. 2Henan Academy of Fishery Sciences, Zhengzhou 450044, China.
  3. 3Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Hunan Academy of Agricultural Sciences, Changsha 410153, China.
  4. 4Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries (Zhejiang Freshwater Fishery Environmental Monitoring Station), Huzhou 313001, China.
  5. 5Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
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Abstract

Folliculogenesis is essential for fish reproduction, but the role of ERK signaling during early primary follicle development in teleosts remains unclear. Here, we reanalyzed and integrated previously published transcriptomic and DNA methylation datasets from four primary follicle subtypes in zebrafish to identify MAPK-related candidate genes. No new sequencing was performed. Additional qRT-PCR and immunohistochemical analyses examined gene expression and the localization of total ERK and JNK proteins, respectively. Zebrafish were also exposed to the ERK inhibitor SCH772984 from 25 to 60 days post-fertilization to assess changes in ovarian development. The reanalysis identified MAPK-related genes with stage-associated differences in expression and DNA methylation. SCH772984 exposure was associated with reduced ovarian ERK phosphorylation and a lower proportion of advanced follicles. These results support a role for ERK signaling in the progression of early ovarian follicles in zebrafish. These observations derive from a single ERK inhibitor at nominal exposure; complementary assays could strengthen the evidence for target specificity.

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Cite

BibTeXRIS
BibTeX
@article{zhang2026mapk,
  title = {{MAPK Signaling Contributes to Primary Follicle Development in Zebrafish (Danio rerio).}},
  author = {Guangjing Zhang and Jing Huang and Xiudan Yuan and Yunpeng Fan and Fangyuan Peng and Wen Fu and Jinhui Liu and Wenbin Liu and Liangyue Peng and Yamei Xiao},
  journal = {Animals : an open access journal from MDPI},
  year = {2026},
  month = sep,
  doi = {10.3390/ani16193078},
  url = {https://europepmc.org/article/MED/42883048},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - MAPK Signaling Contributes to Primary Follicle Development in Zebrafish (Danio rerio).
AU  - Guangjing Zhang
AU  - Jing Huang
AU  - Xiudan Yuan
AU  - Yunpeng Fan
AU  - Fangyuan Peng
AU  - Wen Fu
AU  - Jinhui Liu
AU  - Wenbin Liu
AU  - Liangyue Peng
AU  - Yamei Xiao
PY  - 2026
DA  - 2026/09/30
JO  - Animals : an open access journal from MDPI
DO  - 10.3390/ani16193078
UR  - https://europepmc.org/article/MED/42883048
AB  - Folliculogenesis is essential for fish reproduction, but the role of ERK signaling during early primary follicle development in teleosts remains unclear. Here, we reanalyzed and integrated previously published transcriptomic and DNA methylation datasets from four primary follicle subtypes in zebrafish to identify MAPK-related candidate genes. No new sequencing was performed. Additional qRT-PCR and immunohistochemical analyses examined gene expression and the localization of total ERK and JNK proteins, respectively. Zebrafish were also exposed to the ERK inhibitor SCH772984 from 25 to 60 days post-fertilization to assess changes in ovarian development. The reanalysis identified MAPK-related genes with stage-associated differences in expression and DNA methylation. SCH772984 exposure was associated with reduced ovarian ERK phosphorylation and a lower proportion of advanced follicles. These results support a role for ERK signaling in the progression of early ovarian follicles in zebrafish. These observations derive from a single ERK inhibitor at nominal exposure; complementary assays could strengthen the evidence for target specificity.
SN  - 2076-2615
ER  -