Therapeutic Potential of [(AP)₂M]: A Rationally Designed Polyherbal Formulation for Antidiabetic and Antihyperlipidemic Effects in Alloxan-Induced Diabetic Mice.
- 1Department of Biochemistry and Molecular Biology, Faculty of Science University of Rajshahi Rajshahi Bangladesh.
Abstract
Medicinal plants are widely used in diabetes management due to their multi-targeted bioactive constituents. Phyllanthus emblica, Piper nigrum, Aegle marmelos, Moringa oleifera, and Azadirachta indica possess well-documented antidiabetic properties, providing a scientific basis for developing a rationally designed polyherbal formulation. This study evaluated the basic phytochemical characterization, antioxidant, antidiabetic, antihyperlipidemic, and preliminary toxicity profiles of a polyherbal formulation (designated as [(AP)₂M]) in an alloxan-induced type 1 diabetic mouse model. Polyphenolic (phenolic and flavonoid) contents, antioxidant activity (DPPH & metal-chelating assays), and α-amylase and α-glucosidase inhibitory activities were determined. Acute oral toxicity was assessed according to OECD Guideline 423, with LD₅₀ estimation to establish the preliminary toxicity profile and effective dosing. Diabetic mice received the formulation [(AP)₂M] (30-120 mg/kg) orally for 3 weeks, after which blood glucose, the lipid profile, SGPT, SGOT, and histopathological changes in the liver, kidneys, and pancreas were evaluated. The formulation exhibited substantial phenolic (111.52 ± 0.444 mg GAE/g) and flavonoid (211.30 ± 5.129 mg CAE/g) contents together with strong antioxidant and antidiabetic enzyme inhibitory activity. Acute oral toxicity assessment estimated an LD₅₀ of 900 mg/kg (GHS Category 4). At 120 mg/kg, it reduced blood glucose to a level comparable with glibenclamide and significantly improved lipid profile, SGPT, and SGOT levels (p < 0.001). Histopathological examination demonstrated improvement in pancreatic, hepatic, and renal tissues. Since the formulation demonstrated promising antioxidant, antihyperglycemic, and antihyperlipidemic activities in a diabetic mouse model, it could be a potential alternative for further investigation as a complementary therapeutic approach for diabetes management.
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BibTeX
@article{ahmed2026therapeutic,
title = {{Therapeutic Potential of [(AP)₂M]: A Rationally Designed Polyherbal Formulation for Antidiabetic and Antihyperlipidemic Effects in Alloxan-Induced Diabetic Mice.}},
author = {Md Shakil Ahmed and Md Taohid Hasan and Md Bismillah and M Matiar Rahman and Mohammad Ismail Hossain and Masudul Hasan Khan and Mohammad Amirul Islam},
journal = {Food science \& nutrition},
year = {2026},
month = oct,
doi = {10.1002/fsn3.72520},
url = {https://europepmc.org/article/MED/42948181},
note = {CC BY},
}
RIS
TY - JOUR
TI - Therapeutic Potential of [(AP)₂M]: A Rationally Designed Polyherbal Formulation for Antidiabetic and Antihyperlipidemic Effects in Alloxan-Induced Diabetic Mice.
AU - Md Shakil Ahmed
AU - Md Taohid Hasan
AU - Md Bismillah
AU - M Matiar Rahman
AU - Mohammad Ismail Hossain
AU - Masudul Hasan Khan
AU - Mohammad Amirul Islam
PY - 2026
DA - 2026/10/26
JO - Food science & nutrition
DO - 10.1002/fsn3.72520
UR - https://europepmc.org/article/MED/42948181
AB - Medicinal plants are widely used in diabetes management due to their multi-targeted bioactive constituents. Phyllanthus emblica, Piper nigrum, Aegle marmelos, Moringa oleifera, and Azadirachta indica possess well-documented antidiabetic properties, providing a scientific basis for developing a rationally designed polyherbal formulation. This study evaluated the basic phytochemical characterization, antioxidant, antidiabetic, antihyperlipidemic, and preliminary toxicity profiles of a polyherbal formulation (designated as [(AP)₂M]) in an alloxan-induced type 1 diabetic mouse model. Polyphenolic (phenolic and flavonoid) contents, antioxidant activity (DPPH & metal-chelating assays), and α-amylase and α-glucosidase inhibitory activities were determined. Acute oral toxicity was assessed according to OECD Guideline 423, with LD₅₀ estimation to establish the preliminary toxicity profile and effective dosing. Diabetic mice received the formulation [(AP)₂M] (30-120 mg/kg) orally for 3 weeks, after which blood glucose, the lipid profile, SGPT, SGOT, and histopathological changes in the liver, kidneys, and pancreas were evaluated. The formulation exhibited substantial phenolic (111.52 ± 0.444 mg GAE/g) and flavonoid (211.30 ± 5.129 mg CAE/g) contents together with strong antioxidant and antidiabetic enzyme inhibitory activity. Acute oral toxicity assessment estimated an LD₅₀ of 900 mg/kg (GHS Category 4). At 120 mg/kg, it reduced blood glucose to a level comparable with glibenclamide and significantly improved lipid profile, SGPT, and SGOT levels (p < 0.001). Histopathological examination demonstrated improvement in pancreatic, hepatic, and renal tissues. Since the formulation demonstrated promising antioxidant, antihyperglycemic, and antihyperlipidemic activities in a diabetic mouse model, it could be a potential alternative for further investigation as a complementary therapeutic approach for diabetes management.
SN - 2048-7177
ER -