A high-throughput 96-well plate method to measure human uterine contractility
Abstract
Uterine smooth muscle dysfunction is a common factor in many gynecological conditions, such as endometriosis, adenomyosis, dysmenorrhea, infertility, and labor dystocia. Here, we present a novel high-throughput, optical, low-cost, 96-well plate-based method for measuring myogenic exvivo uterine contractility. We validate this new method on non-pregnant human myometrial samples obtained from hysterectomies by systematically comparing contraction frequency, amplitude, and duration with a standard method using isometric force transducers. We illustrate the potential of this method by screening 8 different compounds, including hormones and pharmacological agents. In particular, we found that antagonists of T-type calcium channels (Z944) and chloride channels (benzbromarone) both decreased contractility in the non-pregnant human uterus. This method will accelerate the pharmacological development of myometrium-specific relaxing agents and provide a powerful tool to investigate the dysregulation of myogenic contractility in gynecological pathologies.
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BibTeX
@misc{emilie2026highthroughput,
title = {{A high-throughput 96-well plate method to measure human uterine contractility}},
author = {Huon Emilie and Rayssac Justine and Lamau Marie-Charlotte and Abo Carole and Dietrich Gauthier and Abrahami Ylann and Kouby Jordan and Adam Julien and Chevalier R. Nicolas},
howpublished = {HAL},
year = {2026},
month = aug,
url = {https://hal.science/hal-05732517v1},
note = {CC BY-NC 4.0},
}
RIS
TY - UNPB
TI - A high-throughput 96-well plate method to measure human uterine contractility
AU - Huon Emilie
AU - Rayssac Justine
AU - Lamau Marie-Charlotte
AU - Abo Carole
AU - Dietrich Gauthier
AU - Abrahami Ylann
AU - Kouby Jordan
AU - Adam Julien
AU - Chevalier R. Nicolas
PY - 2026
DA - 2026/08/31
PB - HAL
UR - https://hal.science/hal-05732517v1
AB - Uterine smooth muscle dysfunction is a common factor in many gynecological conditions, such as endometriosis, adenomyosis, dysmenorrhea, infertility, and labor dystocia. Here, we present a novel high-throughput, optical, low-cost, 96-well plate-based method for measuring myogenic exvivo uterine contractility. We validate this new method on non-pregnant human myometrial samples obtained from hysterectomies by systematically comparing contraction frequency, amplitude, and duration with a standard method using isometric force transducers. We illustrate the potential of this method by screening 8 different compounds, including hormones and pharmacological agents. In particular, we found that antagonists of T-type calcium channels (Z944) and chloride channels (benzbromarone) both decreased contractility in the non-pregnant human uterus. This method will accelerate the pharmacological development of myometrium-specific relaxing agents and provide a powerful tool to investigate the dysregulation of myogenic contractility in gynecological pathologies.
ER -