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Peer-reviewedCC BYNature Neuroscience

Probing inter-areal computations with a two-photon holographic mesoscope

  • Lamiae Abdeladim1,2
  • Uday K. Jagadisan3,4
  • Hyeyoung Shin3,4,5
  • Mora Belén Ogando3,4
  • Hillel Adesnik6,7,8
8 affiliations
  1. 1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. [email protected]
  2. 2Department of Neuroscience, University of California, Berkeley, CA, USA. [email protected]
  3. 3Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
  4. 4Department of Neuroscience, University of California, Berkeley, CA, USA
  5. 5School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
  6. 6Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. [email protected]
  7. 7Department of Neuroscience, University of California, Berkeley, CA, USA. [email protected]
  8. 8The Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA. [email protected]
Open at sourceDOICite

Abstract

Brain computation depends on intricately connected yet highly distributed neural networks. Owing to the absence of the requisite technologies, causally testing fundamental hypotheses on inter-areal processing has remained largely out of reach. Here, we developed a two-photon holographic mesoscope capable of simultaneously reading and writing neural activity patterns with near-single-cell resolution across large regions of the mouse cortex. We demonstrate the precise photoactivation of spatial and temporal sequences of neurons in one or multiple cortical areas while reading out the downstream effects in several other regions. Thus, we have established mesoscale two-photon holographic optogenetics as a platform for mapping functional connectivity and causal interactions across distributed cortical areas with high resolution.

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Cite

BibTeXRIS
BibTeX
@article{abdeladim2026probing,
  title = {{Probing inter-areal computations with a two-photon holographic mesoscope}},
  author = {Lamiae Abdeladim and Uday K. Jagadisan and Hyeyoung Shin and Mora Belén Ogando and Hillel Adesnik},
  journal = {Nature Neuroscience},
  year = {2026},
  month = aug,
  doi = {10.1038/s41593-026-02350-9},
  url = {https://doi.org/10.1038/s41593-026-02350-9},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Probing inter-areal computations with a two-photon holographic mesoscope
AU  - Lamiae Abdeladim
AU  - Uday K. Jagadisan
AU  - Hyeyoung Shin
AU  - Mora Belén Ogando
AU  - Hillel Adesnik
PY  - 2026
DA  - 2026/08/01
JO  - Nature Neuroscience
DO  - 10.1038/s41593-026-02350-9
UR  - https://doi.org/10.1038/s41593-026-02350-9
AB  - Brain computation depends on intricately connected yet highly distributed neural networks. Owing to the absence of the requisite technologies, causally testing fundamental hypotheses on inter-areal processing has remained largely out of reach. Here, we developed a two-photon holographic mesoscope capable of simultaneously reading and writing neural activity patterns with near-single-cell resolution across large regions of the mouse cortex. We demonstrate the precise photoactivation of spatial and temporal sequences of neurons in one or multiple cortical areas while reading out the downstream effects in several other regions. Thus, we have established mesoscale two-photon holographic optogenetics as a platform for mapping functional connectivity and causal interactions across distributed cortical areas with high resolution.
SN  - 1097-6256
SN  - 1546-1726
ER  -