Cell wall-associated proteins from Phaeomoniella chlamydospora trigger calcium-dependent oxidative burst in grapevine cells
Abstract
Esca is a major grapevine trunk disease causing devastating economic losses in vineyards. This complex disease results from trunk colonization by fungi such as Phaeomoniella chlamydospora ( P.ch ). Because the characterization of early molecular events occurring during infection is difficult to perform directly in grapevine trunks, we previously developed a procedure based on stomatal movement to investigate early signaling events during pathogen perception. Assays performed on epidermal peels revealed that cell wall–associated proteins, but not polysaccharides, from P.ch induced stomatal closure in Vitis vinifera . Using the fluorescent probe H 2 DCFDA, we further showed that the perception of these cell wall proteins triggered a rapid accumulation of reactive oxygen species (ROS) in grapevine cells. The addition of diphenyleneiodonium chloride (DPI), an inhibitor of NADPH oxidase–dependent ROS production, prevented the stomatal closure induced by the cell wall proteins. Furthermore, treatment with the calcium (Ca)-chelating agent EGTA blocked both ROS accumulation and stomatal closure triggered by the cell wall proteins, suggesting that the oxidative burst induced by these proteins is dependent on Ca signaling. Altogether, these results provide new insights into the mechanisms underlying grapevine perception and early defense responses against an esca-associated pathogen.
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BibTeX
@article{cruyssen2026cell,
title = {{Cell wall-associated proteins from Phaeomoniella chlamydospora trigger calcium-dependent oxidative burst in grapevine cells}},
author = {Anaïs Vander Cruyssen and Matthias Bidaud and Camille Mourlanne and Ana Romeo-Oliván and Alban Jacques and Olivier Rodrigues},
journal = {Plant Physiology and Biochemistry},
year = {2026},
month = sep,
doi = {10.1016/j.plaphy.2026.111520},
url = {https://hal.science/hal-05738615v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Cell wall-associated proteins from Phaeomoniella chlamydospora trigger calcium-dependent oxidative burst in grapevine cells
AU - Anaïs Vander Cruyssen
AU - Matthias Bidaud
AU - Camille Mourlanne
AU - Ana Romeo-Oliván
AU - Alban Jacques
AU - Olivier Rodrigues
PY - 2026
DA - 2026/09/04
JO - Plant Physiology and Biochemistry
DO - 10.1016/j.plaphy.2026.111520
UR - https://hal.science/hal-05738615v1
AB - Esca is a major grapevine trunk disease causing devastating economic losses in vineyards. This complex disease results from trunk colonization by fungi such as Phaeomoniella chlamydospora ( P.ch ). Because the characterization of early molecular events occurring during infection is difficult to perform directly in grapevine trunks, we previously developed a procedure based on stomatal movement to investigate early signaling events during pathogen perception. Assays performed on epidermal peels revealed that cell wall–associated proteins, but not polysaccharides, from P.ch induced stomatal closure in Vitis vinifera . Using the fluorescent probe H 2 DCFDA, we further showed that the perception of these cell wall proteins triggered a rapid accumulation of reactive oxygen species (ROS) in grapevine cells. The addition of diphenyleneiodonium chloride (DPI), an inhibitor of NADPH oxidase–dependent ROS production, prevented the stomatal closure induced by the cell wall proteins. Furthermore, treatment with the calcium (Ca)-chelating agent EGTA blocked both ROS accumulation and stomatal closure triggered by the cell wall proteins, suggesting that the oxidative burst induced by these proteins is dependent on Ca signaling. Altogether, these results provide new insights into the mechanisms underlying grapevine perception and early defense responses against an esca-associated pathogen.
SN - 0981-9428
SN - 0981-9428
ER -