Integrated Analysis of Grapevine Responses to Winter Pruning Reveals Temperature‐Associated, Polyphenol‐Rich Xylem Occlusions
6 affiliations
- 1EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV Villenave d'Ornon France
- 2Department of Agronomy, Biotechnology and Food Science Univ. Pública de Navarra UPNA Pamplona Spain
- 3Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Umeå Sweden
- 4Institute of Biophysics BOKU University Vienna Austria
- 5Univ. Bordeaux, Bordeaux INP, INRAE, OENO, UMR 1366, ISVV Villenave‐d'Ornon France
- 6Bordeaux Metabolome Facility, MetaboHUB, PHENOME‐EMPHASIS Villenave d'Ornon France
Abstract
Perennial crops are continually wounded throughout cultivation. To limit pathogen infection and tissue dehydration, plants have evolved a range of wound responses that protect damaged tissues. These responses include changes in gene expression and the synthesis of protective compounds such as polyphenols. In addition, plants occlude damaged xylem vessels following wounding and pathogen infection, forming structures such as gels, gums, and tyloses. We explored grapevine responses to pruning. First, we examined the dynamics of xylem vessel occlusion formation at different time points and depths from the wound surface. Second, we characterised transcriptional changes and polyphenol accumulation in response to pruning. Finally, we used Raman microscopy to resolve the spatial chemical composition of xylem vessel occlusions. We found that the winter xylem vessel occlusion response is strongly associated with air temperature. Furthermore, Raman microscopy demonstrated that these occlusions were gel‐ or gum‐like structures enriched in stilbenoids and flavonoids. The accumulation of polyphenols within the occlusions was associated with the up‐regulation of stilbene synthase genes and the accumulation and oligomerization of stilbenes. Overall, this study provides an integrated characterisation of perennial woody tissue responses to winter pruning, combining histological, metabolomic, transcriptomic, and spectroscopic approaches, and highlights a potential regulatory role for temperature in xylem vessel occlusion formation and dynamics.
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BibTeX
@article{camboue2026integrated,
title = {{Integrated Analysis of Grapevine Responses to Winter Pruning Reveals Temperature‐Associated, Polyphenol‐Rich Xylem Occlusions}},
author = {Marilou Camboué and Monica Galar‐Martínez and Grégoire Loupit and Paraskevi Charalambous and Enric Zehraoui and Joseph Tran and Josep Valls Fonayet and Luis Gonzaga Santesteban and Notburga Gierlinger and Sarah Jane Cookson},
journal = {Plant, Cell \& Environment},
year = {2026},
month = sep,
doi = {10.1111/pce.70921},
url = {https://doi.org/10.1111/pce.70921},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Integrated Analysis of Grapevine Responses to Winter Pruning Reveals Temperature‐Associated, Polyphenol‐Rich Xylem Occlusions
AU - Marilou Camboué
AU - Monica Galar‐Martínez
AU - Grégoire Loupit
AU - Paraskevi Charalambous
AU - Enric Zehraoui
AU - Joseph Tran
AU - Josep Valls Fonayet
AU - Luis Gonzaga Santesteban
AU - Notburga Gierlinger
AU - Sarah Jane Cookson
PY - 2026
DA - 2026/09/23
JO - Plant, Cell & Environment
DO - 10.1111/pce.70921
UR - https://doi.org/10.1111/pce.70921
AB - Perennial crops are continually wounded throughout cultivation. To limit pathogen infection and tissue dehydration, plants have evolved a range of wound responses that protect damaged tissues. These responses include changes in gene expression and the synthesis of protective compounds such as polyphenols. In addition, plants occlude damaged xylem vessels following wounding and pathogen infection, forming structures such as gels, gums, and tyloses. We explored grapevine responses to pruning. First, we examined the dynamics of xylem vessel occlusion formation at different time points and depths from the wound surface. Second, we characterised transcriptional changes and polyphenol accumulation in response to pruning. Finally, we used Raman microscopy to resolve the spatial chemical composition of xylem vessel occlusions. We found that the winter xylem vessel occlusion response is strongly associated with air temperature. Furthermore, Raman microscopy demonstrated that these occlusions were gel‐ or gum‐like structures enriched in stilbenoids and flavonoids. The accumulation of polyphenols within the occlusions was associated with the up‐regulation of stilbene synthase genes and the accumulation and oligomerization of stilbenes. Overall, this study provides an integrated characterisation of perennial woody tissue responses to winter pruning, combining histological, metabolomic, transcriptomic, and spectroscopic approaches, and highlights a potential regulatory role for temperature in xylem vessel occlusion formation and dynamics.
SN - 0140-7791
SN - 1365-3040
ER -