Overexpression of the grapevine PGIP1 in tobacco is associated with leaf cell wall pectin modification and glycoprotein changes following Botrytis cinerea infection
Abstract
Introduction: Grapevine PGIP1 (polygalacturonase inhibiting protein 1) expressed in tobacco produces plants with enhanced resistance against B. cinerea infection. Grapevine PGIP1 has been shown to remodel leaf cell walls in transgenic tobacco in the absence of fungal infection. The associated dynamic cell wall changes linked to the mechanism of resistance have not been investigated in the context of fungal infection. Methods: Scanning electron microscopy was used to evaluate infection progression. Monosaccharide composition analysis was used to evaluate cell wall changes as infection progressed. Comprehensive microarray polymer profiling tracked polysaccharide and glycoprotein changes. Results: No difference was observed for lesions between wild-type and transgenic PGIP1 during the first 72 hpi. Rhamnogalacturonan I (RG-I) levels increased at the infection site. The cell wall glycoproteins, arabinogalactan proteins and extensin proteins, show higher signals in the lesion and adjacent tissue in response to Botrytis cinerea infection. Discussion: RG-I and cell wall protein reorganisation in grapevine PGIP1expressing tobacco lines upon infection with Botrytis cinerea suggest cell wall remodelling enhances fungal resistance.
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BibTeX
@article{weiller2026overexpression,
title = {{Overexpression of the grapevine PGIP1 in tobacco is associated with leaf cell wall pectin modification and glycoprotein changes following Botrytis cinerea infection}},
author = {Florent Weiller and Jonatan U Fangel and William G T Willats and Azeddine Driouich and Melané A Vivier and John P Moore},
journal = {Frontiers in Plant Science},
year = {2026},
month = oct,
doi = {10.3389/fpls.2026.1935736},
url = {https://hal.science/hal-05835143v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Overexpression of the grapevine PGIP1 in tobacco is associated with leaf cell wall pectin modification and glycoprotein changes following Botrytis cinerea infection
AU - Florent Weiller
AU - Jonatan U Fangel
AU - William G T Willats
AU - Azeddine Driouich
AU - Melané A Vivier
AU - John P Moore
PY - 2026
DA - 2026/10/21
JO - Frontiers in Plant Science
DO - 10.3389/fpls.2026.1935736
UR - https://hal.science/hal-05835143v1
AB - Introduction: Grapevine PGIP1 (polygalacturonase inhibiting protein 1) expressed in tobacco produces plants with enhanced resistance against B. cinerea infection. Grapevine PGIP1 has been shown to remodel leaf cell walls in transgenic tobacco in the absence of fungal infection. The associated dynamic cell wall changes linked to the mechanism of resistance have not been investigated in the context of fungal infection. Methods: Scanning electron microscopy was used to evaluate infection progression. Monosaccharide composition analysis was used to evaluate cell wall changes as infection progressed. Comprehensive microarray polymer profiling tracked polysaccharide and glycoprotein changes. Results: No difference was observed for lesions between wild-type and transgenic PGIP1 during the first 72 hpi. Rhamnogalacturonan I (RG-I) levels increased at the infection site. The cell wall glycoproteins, arabinogalactan proteins and extensin proteins, show higher signals in the lesion and adjacent tissue in response to Botrytis cinerea infection. Discussion: RG-I and cell wall protein reorganisation in grapevine PGIP1expressing tobacco lines upon infection with Botrytis cinerea suggest cell wall remodelling enhances fungal resistance.
SN - 1664-462X
ER -