Dietary Cupplant (Silphium perfoliatum L.) flavonoid supplementation is associated with changes in cecal microbial diversity and community structure in broiler chickens.
4 affiliations
- 1Department of Animal Nutrition and Feed Science, College of Animal Sciences & Technology, Jilin Agricultural University, Changchun, 130118, China; Department of Animal Science, Faculty of Agriculture, National University of Lesotho, Roma, Maseru, 180, Lesotho.
- 2Department of Animal Nutrition and Feed Science, College of Animal Sciences & Technology, Jilin Agricultural University, Changchun, 130118, China.
- 3Jilin Provincial Animal Husbandry Station, Jilin, China.
- 4Department of Animal Nutrition and Feed Science, College of Animal Sciences & Technology, Jilin Agricultural University, Changchun, 130118, China. Electronic address: [email protected].
Abstract
Cupplant (Silphium perfoliatum L.) contains flavonoids that may influence the intestinal microbiota, but their effects in broilers remain poorly defined. We examined cecal microbial responses to dietary Cupplant flavonoids. Two hundred forty one-day-old broilers were assigned to four diets containing 0 (CH), 100 (S1), 200 (S2), or 300 (S3) mg/kg flavonoid extract, with five replicate cages per treatment. At day 42, cecal digesta from six birds per treatment were characterized using 16S rRNA gene amplicon sequencing; one cage per treatment contributed two sampled birds. QIIME2/DADA2 processing yielded 1,601,918 retained reads and 2,735 amplicon sequence variants. Observed ASVs, Chao1 richness, and Shannon diversity were higher in S3 than CH (adjusted P < 0.05), although responses were non-monotonic. Global Bray-Curtis PERMANOVA showed treatment-associated differences in community composition (pseudo-F(3,20) = 2.997, R² = 0.310, P = 0.001), while PERMDISP was non-significant (P = 0.168). All pairwise PERMANOVA comparisons remained significant after multiplicity correction. False-discovery-rate (FDR)-controlled metagenomeSeq identified six genera in the CH-S1 comparison and three in CH-S3, with Escherichia-Shigella, Desulfovibrio, and Anaerotignum recurring in both contrasts; CH-S2 had no FDR-significant genera. Reconstructed FDR-controlled networks retained only 6.8-12.0 % of nominal edges, and βNTI, NST, and iCAMP results did not support a monotonic dose-dependent shift in community assembly. Descriptive final body weight and average daily gain values were similar across treatments, but cage-level growth inference was not possible from the archived data. Cupplant flavonoid supplementation was associated with non-linear changes in cecal microbial diversity and composition, warranting confirmation in cage-aware, adequately powered studies.
Related
- Early-Life Oat Supplementation Is Associated with Altered Cecal Microbiota and Microbial Metabolic Profiles and Reduced Campylobacter jejuni Colonization in Broiler Chickens.
- Plant genetic and root‐associated microbial diversity modulate Lactuca sativa responsiveness to a soil inoculum under phosphate deficiency
- Editorial: Salt-stress-relieving and growth-promoting microbial diversity and its interaction mechanism with plant rhizosphere
- Microbial diversity, safety, and quality challenges of several commercial plant-based meat products in Thailand
- Influence of Dietary Rumen-Protected Choline and Nicotinic Acid Supplementation on Growth Outcomes and Meat Tenderness, Rumen Microbial Composition, and Serum Metabolomics of Late-Fattening Beef Cattle.
BibTeX
@article{makatjane2026dietary,
title = {{Dietary Cupplant (Silphium perfoliatum L.) flavonoid supplementation is associated with changes in cecal microbial diversity and community structure in broiler chickens.}},
author = {Kabelo Anthony Makatjane and Jiayin Xu and Wei Guo and Guanglong Liu and Yuhan Wang and Hongzhi Lin and Zhang Fang and Tengyuqi Li and Haizhu Zhou},
journal = {Poultry science},
year = {2026},
month = oct,
doi = {10.1016/j.psj.2026.107876},
url = {https://europepmc.org/article/MED/42849234},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Dietary Cupplant (Silphium perfoliatum L.) flavonoid supplementation is associated with changes in cecal microbial diversity and community structure in broiler chickens.
AU - Kabelo Anthony Makatjane
AU - Jiayin Xu
AU - Wei Guo
AU - Guanglong Liu
AU - Yuhan Wang
AU - Hongzhi Lin
AU - Zhang Fang
AU - Tengyuqi Li
AU - Haizhu Zhou
PY - 2026
DA - 2026/10/05
JO - Poultry science
DO - 10.1016/j.psj.2026.107876
UR - https://europepmc.org/article/MED/42849234
AB - Cupplant (Silphium perfoliatum L.) contains flavonoids that may influence the intestinal microbiota, but their effects in broilers remain poorly defined. We examined cecal microbial responses to dietary Cupplant flavonoids. Two hundred forty one-day-old broilers were assigned to four diets containing 0 (CH), 100 (S1), 200 (S2), or 300 (S3) mg/kg flavonoid extract, with five replicate cages per treatment. At day 42, cecal digesta from six birds per treatment were characterized using 16S rRNA gene amplicon sequencing; one cage per treatment contributed two sampled birds. QIIME2/DADA2 processing yielded 1,601,918 retained reads and 2,735 amplicon sequence variants. Observed ASVs, Chao1 richness, and Shannon diversity were higher in S3 than CH (adjusted P < 0.05), although responses were non-monotonic. Global Bray-Curtis PERMANOVA showed treatment-associated differences in community composition (pseudo-F(3,20) = 2.997, R² = 0.310, P = 0.001), while PERMDISP was non-significant (P = 0.168). All pairwise PERMANOVA comparisons remained significant after multiplicity correction. False-discovery-rate (FDR)-controlled metagenomeSeq identified six genera in the CH-S1 comparison and three in CH-S3, with Escherichia-Shigella, Desulfovibrio, and Anaerotignum recurring in both contrasts; CH-S2 had no FDR-significant genera. Reconstructed FDR-controlled networks retained only 6.8-12.0 % of nominal edges, and βNTI, NST, and iCAMP results did not support a monotonic dose-dependent shift in community assembly. Descriptive final body weight and average daily gain values were similar across treatments, but cage-level growth inference was not possible from the archived data. Cupplant flavonoid supplementation was associated with non-linear changes in cecal microbial diversity and composition, warranting confirmation in cage-aware, adequately powered studies.
SN - 0032-5791
SN - 1525-3171
ER -