Development of a New Bioadhesive Gel in the Prevention of Bacterial Biofilms on Dental Implants: An In Vitro Study
- 1Department of Stomatology, Faculty of Dentistry University of Seville Seville Spain
- 2Faculty of Health Sciences Universidad Europea de Valencia Valencia Spain
- 3LACER Medical Department Barcelona Spain
Abstract
Objectives Peri‐implantitis is a reality that clinicians face in their daily practice. Current evidence discusses the applicability of bacteriostatic or bactericidal methods in order to minimize the colonization of bacteria, which entails controversy. In this scenario there arises a need to develop surfaces that minimize bacteria's appetite for them. Methods An in vitro microbiological study was conducted to assess the effect of a chlorhexidine‐based bioadhesive gel on the growth and adhesion of a simplified peri‐implantitis–associated microbial consortium. Grade IV titanium disks with three surface types (machined, sandblasted, and ContacTi) were incubated with the consortium under anaerobic conditions for 10 days, with and without gel treatment. Microbial viability was evaluated by colony‐forming unit (CFU) counts, and biofilm structure was analyzed by scanning electron microscopy. Microbial community composition was assessed using 16S rRNA gene amplicon sequencing. Results After 10 days, gel‐treated implants showed complete inhibition of cultivable microorganisms, with CFU values reduced below the detection limit on all surfaces, compared with untreated implants (p < 0.05). In untreated samples, ContacTi surfaces exhibited higher bacterial viability than machined and sandblasted surfaces. Metataxonomic analysis revealed a marked reduction in peri‐implant pathogens, particularly Porphyromonas gingivalis, whose relative abundance decreased from approximately 20% in the initial inoculum to ~1% after incubation. Conclusions The bioadhesive gel demonstrated a quantitatively significant antimicrobial effect, preventing biofilm formation on all tested implant surfaces after 10 days.
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BibTeX
@article{bausdominguez2026development,
title = {{Development of a New Bioadhesive Gel in the Prevention of Bacterial Biofilms on Dental Implants: An In Vitro Study}},
author = {María Baus‐Domínguez and Paula Hermida‐Cabrera and Javier Pascual and Fernando Vivancos‐Cuadras and María‐Ángeles Serrera‐Figallo and José‐Luis Gutiérrez‐Pérez and Daniel Torres‐Lagares},
journal = {Clinical and Experimental Dental Research},
year = {2026},
month = oct,
doi = {10.1002/cre2.70422},
url = {https://doi.org/10.1002/cre2.70422},
note = {CC BY},
}
RIS
TY - JOUR
TI - Development of a New Bioadhesive Gel in the Prevention of Bacterial Biofilms on Dental Implants: An In Vitro Study
AU - María Baus‐Domínguez
AU - Paula Hermida‐Cabrera
AU - Javier Pascual
AU - Fernando Vivancos‐Cuadras
AU - María‐Ángeles Serrera‐Figallo
AU - José‐Luis Gutiérrez‐Pérez
AU - Daniel Torres‐Lagares
PY - 2026
DA - 2026/10/30
JO - Clinical and Experimental Dental Research
DO - 10.1002/cre2.70422
UR - https://doi.org/10.1002/cre2.70422
AB - Objectives Peri‐implantitis is a reality that clinicians face in their daily practice. Current evidence discusses the applicability of bacteriostatic or bactericidal methods in order to minimize the colonization of bacteria, which entails controversy. In this scenario there arises a need to develop surfaces that minimize bacteria's appetite for them. Methods An in vitro microbiological study was conducted to assess the effect of a chlorhexidine‐based bioadhesive gel on the growth and adhesion of a simplified peri‐implantitis–associated microbial consortium. Grade IV titanium disks with three surface types (machined, sandblasted, and ContacTi) were incubated with the consortium under anaerobic conditions for 10 days, with and without gel treatment. Microbial viability was evaluated by colony‐forming unit (CFU) counts, and biofilm structure was analyzed by scanning electron microscopy. Microbial community composition was assessed using 16S rRNA gene amplicon sequencing. Results After 10 days, gel‐treated implants showed complete inhibition of cultivable microorganisms, with CFU values reduced below the detection limit on all surfaces, compared with untreated implants (p < 0.05). In untreated samples, ContacTi surfaces exhibited higher bacterial viability than machined and sandblasted surfaces. Metataxonomic analysis revealed a marked reduction in peri‐implant pathogens, particularly Porphyromonas gingivalis, whose relative abundance decreased from approximately 20% in the initial inoculum to ~1% after incubation. Conclusions The bioadhesive gel demonstrated a quantitatively significant antimicrobial effect, preventing biofilm formation on all tested implant surfaces after 10 days.
SN - 2057-4347
ER -