Genomic epidemiology of multidrug-resistant and non-multidrug-resistant clinical Salmonella enterica isolates in Guangdong, China
5 affiliations
- 1Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China
- 2School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China
- 3Guangdong Provincial Key Laboratory of Pathogen Detection for Emerging Infectious Disease Response, Guangzhou, China
- 4Guangdong Workstation for Emerging Infectious Disease Control and Prevention, Chinese Academy of Medical Sciences, Guangzhou, China
- 5School of Public Health, Southern Medical University, Guangzhou, China
Abstract
Salmonella enterica (S. enterica) is a major foodborne pathogen that threatens human health. Multidrug resistance (MDR) has complicated the prevention and treatment of salmonellosis. The present study aimed to characterize the antimicrobial resistance patterns, population structure, and genomic features of clinical S. enterica isolates from Guangdong, China. Among 4413 isolates collected between 2022 and 2024, 1084 underwent antimicrobial susceptibility testing and 170 were further analyzed by whole-genome sequencing. MDR was defined as resistance to at least three antimicrobial classes and was identified in 71.13% of the tested isolates. Genomic analyses included serotyping, multilocus sequence typing, resistance and virulence gene analyses, and core-genome phylogenetic analysis. XGBoost and LASSO were used to prioritize genomic features associated with MDR phenotype. Serovar I 4,[5],12:i:- was the predominant serovar among MDR isolates, while ST34 and ST19 were the most common sequence types. Compared with non-MDR isolates, MDR isolates more frequently carried several resistance genes, including CTX-M-55, qnrS1, tet(A), floR, sul2, sul3, and TEM-1. Differences in virulence gene content were also observed between the two groups. Both XGBoost and LASSO consistently prioritized floR and CTX-M-55 as candidate genomic features associated with the MDR phenotype. Overall, MDR and non-MDR clinical S. enterica isolates from Guangdong differed markedly in lineage distribution and gene carriage, providing a genomic basis for regional surveillance of MDR S. enterica.
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BibTeX
@article{chen2026genomic,
title = {{Genomic epidemiology of multidrug-resistant and non-multidrug-resistant clinical Salmonella enterica isolates in Guangdong, China}},
author = {Sitong Chen and Sitong Chen and Jiajun Liu and Jiajun Liu and Jiajun Liu and Dongmei He and Dongmei He and Dongmei He and Bojun Wang and Ouyang Fangzhu and Ouyang Fangzhu and Ouyang Fangzhu and Yahui Shi and Yahui Shi and Yahui Shi and Bixia Ke and Bixia Ke and Bixia Ke and Baisheng Li and Baisheng Li and Baisheng Li},
journal = {Frontiers in Cellular and Infection Microbiology},
year = {2026},
month = oct,
doi = {10.3389/fcimb.2026.1852160},
url = {https://doi.org/10.3389/fcimb.2026.1852160},
note = {CC BY},
}
RIS
TY - JOUR
TI - Genomic epidemiology of multidrug-resistant and non-multidrug-resistant clinical Salmonella enterica isolates in Guangdong, China
AU - Sitong Chen
AU - Sitong Chen
AU - Jiajun Liu
AU - Jiajun Liu
AU - Jiajun Liu
AU - Dongmei He
AU - Dongmei He
AU - Dongmei He
AU - Bojun Wang
AU - Ouyang Fangzhu
AU - Ouyang Fangzhu
AU - Ouyang Fangzhu
AU - Yahui Shi
AU - Yahui Shi
AU - Yahui Shi
AU - Bixia Ke
AU - Bixia Ke
AU - Bixia Ke
AU - Baisheng Li
AU - Baisheng Li
AU - Baisheng Li
PY - 2026
DA - 2026/10/23
JO - Frontiers in Cellular and Infection Microbiology
DO - 10.3389/fcimb.2026.1852160
UR - https://doi.org/10.3389/fcimb.2026.1852160
AB - Salmonella enterica (S. enterica) is a major foodborne pathogen that threatens human health. Multidrug resistance (MDR) has complicated the prevention and treatment of salmonellosis. The present study aimed to characterize the antimicrobial resistance patterns, population structure, and genomic features of clinical S. enterica isolates from Guangdong, China. Among 4413 isolates collected between 2022 and 2024, 1084 underwent antimicrobial susceptibility testing and 170 were further analyzed by whole-genome sequencing. MDR was defined as resistance to at least three antimicrobial classes and was identified in 71.13% of the tested isolates. Genomic analyses included serotyping, multilocus sequence typing, resistance and virulence gene analyses, and core-genome phylogenetic analysis. XGBoost and LASSO were used to prioritize genomic features associated with MDR phenotype. Serovar I 4,[5],12:i:- was the predominant serovar among MDR isolates, while ST34 and ST19 were the most common sequence types. Compared with non-MDR isolates, MDR isolates more frequently carried several resistance genes, including CTX-M-55, qnrS1, tet(A), floR, sul2, sul3, and TEM-1. Differences in virulence gene content were also observed between the two groups. Both XGBoost and LASSO consistently prioritized floR and CTX-M-55 as candidate genomic features associated with the MDR phenotype. Overall, MDR and non-MDR clinical S. enterica isolates from Guangdong differed markedly in lineage distribution and gene carriage, providing a genomic basis for regional surveillance of MDR S. enterica.
SN - 2235-2988
ER -