Chemical and Proteomic Characterization of Bioactive Protein-Enriched Fraction from Schizophyllum commune with Anticancer Activity in HeLa Cells
- 1King Mongkut’s University of Technology North Bangkok , , ,
- 2Kasetsart University , , ,
Abstract
Schizophyllum commune, commonly known as the split gill mushroom, is an edible fungus recognized as a source of bioactive compounds with potential applications in the food and biotechnology industries. In this study, protein-enriched fractions were isolated from the fruiting bodies of S. commune using ion-exchange chromatography coupled with a bioassay-guided approach. Cytotoxic activity was evaluated in HeLa cells using MTT and colony formation assays. Among the fractions, the protein-enriched fraction F2 exhibited antiproliferative activity with an IC50 value of 0.37 μg/mL and significantly inhibited colony formation, while showing minimal effects on normal HaCaT cells. Flow cytometry and fluorescence staining further supported the involvement of apoptosis in F2-treated HeLa cells. Chemical and proteomic characterization of the protein-enriched fraction F2 by SDS-PAGE and LC–MS/MS identified 64 protein groups. Gene ontology analysis revealed proteins involved in diverse biological processes, while physicochemical profiling demonstrated a heterogeneous protein composition with isoelectric points ranging from 4 to 7. These findings provide insight into the chemical and proteomic composition of protein-enriched fraction F2. The results support further investigation of S. commune-derived protein-enriched fractions as a source of bioactive proteins, particularly in bioactivity-guided fractionation studies.
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BibTeX
@article{sedtananun2026chemical,
title = {{Chemical and Proteomic Characterization of Bioactive Protein-Enriched Fraction from Schizophyllum commune with Anticancer Activity in HeLa Cells}},
author = {Saranya Sedtananun and Lueacha Tabtimmai and Chanapat Butsukon and Chanikan Sonklin and Kiattawee Choowongkomon},
journal = {ACS omega},
year = {2026},
month = oct,
doi = {10.1021/acsomega.6c03766},
url = {https://europepmc.org/article/PMC/PMC13670856},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Chemical and Proteomic Characterization of Bioactive Protein-Enriched Fraction from Schizophyllum commune with Anticancer Activity in HeLa Cells
AU - Saranya Sedtananun
AU - Lueacha Tabtimmai
AU - Chanapat Butsukon
AU - Chanikan Sonklin
AU - Kiattawee Choowongkomon
PY - 2026
DA - 2026/10/01
JO - ACS omega
DO - 10.1021/acsomega.6c03766
UR - https://europepmc.org/article/PMC/PMC13670856
AB - Schizophyllum commune, commonly known as the split gill mushroom, is an edible fungus recognized as a source of bioactive compounds with potential applications in the food and biotechnology industries. In this study, protein-enriched fractions were isolated from the fruiting bodies of S. commune using ion-exchange chromatography coupled with a bioassay-guided approach. Cytotoxic activity was evaluated in HeLa cells using MTT and colony formation assays. Among the fractions, the protein-enriched fraction F2 exhibited antiproliferative activity with an IC50 value of 0.37 μg/mL and significantly inhibited colony formation, while showing minimal effects on normal HaCaT cells. Flow cytometry and fluorescence staining further supported the involvement of apoptosis in F2-treated HeLa cells. Chemical and proteomic characterization of the protein-enriched fraction F2 by SDS-PAGE and LC–MS/MS identified 64 protein groups. Gene ontology analysis revealed proteins involved in diverse biological processes, while physicochemical profiling demonstrated a heterogeneous protein composition with isoelectric points ranging from 4 to 7. These findings provide insight into the chemical and proteomic composition of protein-enriched fraction F2. The results support further investigation of S. commune-derived protein-enriched fractions as a source of bioactive proteins, particularly in bioactivity-guided fractionation studies.
SN - 2470-1343
ER -