Peer-reviewedCC BYSTAR protocols
Protocol for quantifying chromatin DNA replication by multiplex labeling and single-molecule localization microscopy.
4 affiliations
- 1National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China; State Key Laboratory of Membrane Biology & Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing 100871, China. Electronic address: [email protected].
- 2National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China; State Key Laboratory of Membrane Biology & Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing 100871, China; School of Life Sciences, Peking University, Beijing 100871, China. Electronic address: [email protected].
- 3National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China; State Key Laboratory of Membrane Biology & Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing 100871, China; School of Life Sciences, Peking University, Beijing 100871, China.
- 4School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia. Electronic address: [email protected].
Abstract
We present a protocol for imaging and quantifying chromatin DNA replication-associated signals in synchronized mammalian cells. We describe steps for cell-cycle enrichment, nucleoside analog-based metabolic labeling, and fluorescence detection. We then detail the procedure for dual-color STORM imaging and SR-Tesseler-based cluster analysis. It can be adapted to distinct questions in DNA replication biology by modifying synchronization schemes, pulse durations, labeling strategies, fluorescence detection channels, or analysis parameters. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.
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BibTeX
@article{xiao2026protocol,
title = {{Protocol for quantifying chromatin DNA replication by multiplex labeling and single-molecule localization microscopy.}},
author = {Zhiguang Xiao and Xiaodong Guan and Yujie Sun and Mengling Zhang and Qian Peter Su},
journal = {STAR protocols},
year = {2026},
month = oct,
doi = {10.1016/j.xpro.2026.104882},
url = {https://europepmc.org/article/MED/42842375},
note = {CC BY},
}
RIS
TY - JOUR
TI - Protocol for quantifying chromatin DNA replication by multiplex labeling and single-molecule localization microscopy.
AU - Zhiguang Xiao
AU - Xiaodong Guan
AU - Yujie Sun
AU - Mengling Zhang
AU - Qian Peter Su
PY - 2026
DA - 2026/10/07
JO - STAR protocols
DO - 10.1016/j.xpro.2026.104882
UR - https://europepmc.org/article/MED/42842375
AB - We present a protocol for imaging and quantifying chromatin DNA replication-associated signals in synchronized mammalian cells. We describe steps for cell-cycle enrichment, nucleoside analog-based metabolic labeling, and fluorescence detection. We then detail the procedure for dual-color STORM imaging and SR-Tesseler-based cluster analysis. It can be adapted to distinct questions in DNA replication biology by modifying synchronization schemes, pulse durations, labeling strategies, fluorescence detection channels, or analysis parameters. For complete details on the use and execution of this protocol, please refer to Zhang et al.¹.
SN - 2666-1667
ER -