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Peer-reviewedCC BY-NC-NDNature communications

Three-dimensional chromatin architecture is rapidly restored after DNA replication.

  • Tyler K Fenstermaker1,2
  • Svetlana Petruk3
  • Alexander Mazo4
4 affiliations
  1. 1Department of Biochemistry and Molecular Biology, Sidney Kimmel Medical College, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA. [email protected].
  2. 2Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, 19104, USA. [email protected].
  3. 3Department of Biochemistry and Molecular Biology, Sidney Kimmel Medical College, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA.
  4. 4Department of Biochemistry and Molecular Biology, Sidney Kimmel Medical College, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, 19107, USA. [email protected].
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Abstract

Studies of post-replicative chromatin exclusively focus on the kinetics of protein binding after DNA replication to understand maintenance of transcriptional programs. Here we investigated both chromatin-bound factors and the three-dimensional architecture of the post-replicative genome in human cells. We found that transcription factors and chromatin looping proteins rapidly associate with nascent DNA. We discovered that the architectural factors, CTCF and YY1, bind to their target sequences immediately following DNA replication, and that chromatin loops from 39 to 425 kb re-form within 10 min of DNA replication. This suggests that the 3D structure of chromatin is at most briefly disrupted by DNA replication and quickly restores following replisome passage, providing insight into an unaddressed question of the structure of post-replicated chromatin. Our results suggest that all components of the transcriptional network and tertiary chromatin structure are restored shortly after replication, which is consistent with previously detected fast resumption of transcription on daughter DNA strands.

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Cite

BibTeXRIS
BibTeX
@article{fenstermaker2026threedimensional,
  title = {{Three-dimensional chromatin architecture is rapidly restored after DNA replication.}},
  author = {Tyler K Fenstermaker and Svetlana Petruk and Alexander Mazo},
  journal = {Nature communications},
  year = {2026},
  month = sep,
  doi = {10.1038/s41467-026-77901-6},
  url = {https://europepmc.org/article/MED/42893985},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Three-dimensional chromatin architecture is rapidly restored after DNA replication.
AU  - Tyler K Fenstermaker
AU  - Svetlana Petruk
AU  - Alexander Mazo
PY  - 2026
DA  - 2026/09/16
JO  - Nature communications
DO  - 10.1038/s41467-026-77901-6
UR  - https://europepmc.org/article/MED/42893985
AB  - Studies of post-replicative chromatin exclusively focus on the kinetics of protein binding after DNA replication to understand maintenance of transcriptional programs. Here we investigated both chromatin-bound factors and the three-dimensional architecture of the post-replicative genome in human cells. We found that transcription factors and chromatin looping proteins rapidly associate with nascent DNA. We discovered that the architectural factors, CTCF and YY1, bind to their target sequences immediately following DNA replication, and that chromatin loops from 39 to 425 kb re-form within 10 min of DNA replication. This suggests that the 3D structure of chromatin is at most briefly disrupted by DNA replication and quickly restores following replisome passage, providing insight into an unaddressed question of the structure of post-replicated chromatin. Our results suggest that all components of the transcriptional network and tertiary chromatin structure are restored shortly after replication, which is consistent with previously detected fast resumption of transcription on daughter DNA strands.
SN  - 2041-1723
ER  -