Decoding grapevine elicitation responses through cell culture metabolomics
Abstract
Plant cell culture systems offer a reproducible and controlled platform for studying stress responses in grapevine. However, the extent to which grapevine cell cultures capture inducible metabolic features observed in organ-level systems remains insufficiently explored. Here, we established cell suspension cultures (CSCs) from two grapevine cultivars with contrasting reported disease susceptibility backgrounds, Cabernet Sauvignon and Chambourcin, and compared their jasmonic acid (JA)-induced metabolic responses with those observed in detached leaves. CSCs and detached leaves were elicited with JA, and their metabolic profiles were assessed at 2 and 5 days post-elicitation using Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in negative ion mode. Putatively annotated metabolite candidates revealed cultivar- and time-dependent responses in both systems. Although CSCs and leaves retained distinct global metabolic profiles, a partial functional convergence was observed in selected categories, particularly oxidative stress defense, stress signaling, and secondary metabolism. These results support the use of grapevine CSCs as complementary platforms to investigate selected inducible stress responses, while highlighting the need for further validation in intact plants and targeted metabolite confirmation.
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BibTeX
@article{paes2026decoding,
title = {{Decoding grapevine elicitation responses through cell culture metabolomics}},
author = {Maria Paes and João Proença Pereira and Catarina Paiva-Silva and Nuno Domingues and Jorge Cunha and Jasmine Hertzog and Vincent Carré and Andreia Figueiredo and Marisa Maia and Rita B Santos},
journal = {BMC Plant Biology},
year = {2026},
month = jun,
doi = {10.1186/s12870-026-09217-y},
url = {https://hal.univ-lorraine.fr/hal-05740520v1},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Decoding grapevine elicitation responses through cell culture metabolomics
AU - Maria Paes
AU - João Proença Pereira
AU - Catarina Paiva-Silva
AU - Nuno Domingues
AU - Jorge Cunha
AU - Jasmine Hertzog
AU - Vincent Carré
AU - Andreia Figueiredo
AU - Marisa Maia
AU - Rita B Santos
PY - 2026
DA - 2026/06/22
JO - BMC Plant Biology
DO - 10.1186/s12870-026-09217-y
UR - https://hal.univ-lorraine.fr/hal-05740520v1
AB - Plant cell culture systems offer a reproducible and controlled platform for studying stress responses in grapevine. However, the extent to which grapevine cell cultures capture inducible metabolic features observed in organ-level systems remains insufficiently explored. Here, we established cell suspension cultures (CSCs) from two grapevine cultivars with contrasting reported disease susceptibility backgrounds, Cabernet Sauvignon and Chambourcin, and compared their jasmonic acid (JA)-induced metabolic responses with those observed in detached leaves. CSCs and detached leaves were elicited with JA, and their metabolic profiles were assessed at 2 and 5 days post-elicitation using Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in negative ion mode. Putatively annotated metabolite candidates revealed cultivar- and time-dependent responses in both systems. Although CSCs and leaves retained distinct global metabolic profiles, a partial functional convergence was observed in selected categories, particularly oxidative stress defense, stress signaling, and secondary metabolism. These results support the use of grapevine CSCs as complementary platforms to investigate selected inducible stress responses, while highlighting the need for further validation in intact plants and targeted metabolite confirmation.
SN - 1471-2229
ER -