Application of a light emitting diode based autofluorescence removal system to improve fluorescence imaging…

Abstract

Autofluorescence derived from plant tissues, originating from cell walls, chloroplasts, and various endogenous pigments, often interferes with fluorescence imaging and hampers quantitative analysis. Although numerous fluorescence-based techniques have been developed for plant cell studies, autofluorescence remains a persistent technical obstacle. An LED-based autofluorescence removal system (TiYO), originally developed for animal neural tissues, has recently been shown to selectively reduce tissue autofluorescence without compromising specific fluorescence signals. In this study, we examined whether this system can be applied to plant materials.

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BibTeX

@article{kuriyama2026application,
  title = {{Application of a light emitting diode based autofluorescence removal system to improve fluorescence imaging in plant tissues}},
  author = {Akira Kuriyama and Iyo Li and Ryo Terajima and Hiroto Kitano and Tomoko Abe and Aki Makanae and Mitsuru Yashiro and Shigenobu Toné},
  journal = {Plant Biotechnology},
  year = {2026},
  month = sep,
  doi = {10.5511/plantbiotechnology.26.0527a},
  url = {https://www.jstage.jst.go.jp/article/plantbiotechnology/advpub/0/advpub_26.0527a/_article},
  note = {CC BY},
}

RIS

TY  - JOUR
TI  - Application of a light emitting diode based autofluorescence removal system to improve fluorescence imaging in plant tissues
AU  - Akira Kuriyama
AU  - Iyo Li
AU  - Ryo Terajima
AU  - Hiroto Kitano
AU  - Tomoko Abe
AU  - Aki Makanae
AU  - Mitsuru Yashiro
AU  - Shigenobu Toné
PY  - 2026
DA  - 2026/09/17
JO  - Plant Biotechnology
DO  - 10.5511/plantbiotechnology.26.0527a
UR  - https://www.jstage.jst.go.jp/article/plantbiotechnology/advpub/0/advpub_26.0527a/_article
AB  - Autofluorescence derived from plant tissues, originating from cell walls, chloroplasts, and various endogenous pigments, often interferes with fluorescence imaging and hampers quantitative analysis. Although numerous fluorescence-based techniques have been developed for plant cell studies, autofluorescence remains a persistent technical obstacle. An LED-based autofluorescence removal system (TiYO), originally developed for animal neural tissues, has recently been shown to selectively reduce tissue autofluorescence without compromising specific fluorescence signals. In this study, we examined whether this system can be applied to plant materials.
SN  - 1347-6114
SN  - 1342-4580
ER  -

Abstract

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