Peer-reviewedCC BY 4.0Biochemistry and Biophysics Reports
Addressing complex autofluorescence signatures in solid tissue samples to enhance full spectrum flow cytometry of non-immune cells
- 1Centre for Microvascular Research, William Harvey Research Institute, Queen Mary University of London, EC1M 6BQ, United Kingdom
- 2Flow Cytometry Core Facility, Barts Cancer Institute, Queen Mary University of London, London, EC1M 6BQ, United Kingdom
Abstract
FSFC is an emerging technology that can greatly enhance our understanding of the single-cell proteomic landscape. We demonstrate that this application is applicable to cells derived from solid tissues which have traditionally been hampered by their complex autofluorescence signatures and lack of optimized tools for non-immune cells. We present a protocol and discuss key controls that minimize the impact of unmixing errors, enabling us to resolve multiple EC subpopulations isolated from different tissues in models of chronic tissue injury.
Research topic(s): vascular biology, cell heterogeneity, full spectrum flow cytometry.
Related
- Gut microbiota composition and dysbiosis signatures associated with complex gastrointestinal and urological conditions in neonates: a case series study
- Application of a light emitting diode based autofluorescence removal system to improve fluorescence imaging in plant tissues
- Single-shot light-field microscopy captures delivery-dependent dye and autofluorescence patterns in Caenorhabditis elegans
- Metabolic-State-Dependent Magnetic Field Effects on Endogenous Flavin Autofluorescence in Escherichia coli
- Wide-Field Fundus Autofluorescence Patterns in a Polish Cohort of Genotyped Patients with Phenotype of Retinitis Pigmentosa, Usher Syndrome and Cone–Rod Dystrophy
BibTeX
@article{gkantsinikoudi2026addressing,
title = {{Addressing complex autofluorescence signatures in solid tissue samples to enhance full spectrum flow cytometry of non-immune cells}},
author = {Christina Gkantsinikoudi and Manuela Terranova-Barberio and Neil Dufton},
journal = {Biochemistry and Biophysics Reports},
year = {2026},
month = oct,
doi = {10.1016/j.bbrep.2026.102845},
url = {https://doi.org/10.1016/j.bbrep.2026.102845},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Addressing complex autofluorescence signatures in solid tissue samples to enhance full spectrum flow cytometry of non-immune cells
AU - Christina Gkantsinikoudi
AU - Manuela Terranova-Barberio
AU - Neil Dufton
PY - 2026
DA - 2026/10/17
JO - Biochemistry and Biophysics Reports
DO - 10.1016/j.bbrep.2026.102845
UR - https://doi.org/10.1016/j.bbrep.2026.102845
AB - FSFC is an emerging technology that can greatly enhance our understanding of the single-cell proteomic landscape. We demonstrate that this application is applicable to cells derived from solid tissues which have traditionally been hampered by their complex autofluorescence signatures and lack of optimized tools for non-immune cells. We present a protocol and discuss key controls that minimize the impact of unmixing errors, enabling us to resolve multiple EC subpopulations isolated from different tissues in models of chronic tissue injury. Research topic(s): vascular biology, cell heterogeneity, full spectrum flow cytometry.
SN - 2405-5808
ER -