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Peer-reviewedCC BY-NC-ND 4.0ACS Omega

Single-Excitation, Orthogonally Encoded Quantum Dot Probes for Dual-Mode Multiplexed Biosensing

  • Heshan Yu1,2
  • Yan Li2
  • Rong Yang2
  • Tingting Zhang1
  • Shaoqing Zhang1
  • Xudong He1
  • Jing Ping1
  • Yongjv Yang1
  • Lijian Pang1
  1. 1Liaoning University of Traditional Chinese Medicine Affiliated Hospital , , , ,
  2. 2Liaoning University of Traditional Chinese Medicine , , , ,
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Abstract

Simultaneous quantitative detection of multiple rheumatoid arthritis (RA) biomarkers is often constrained by the need for separate analytical platforms and complex instrumentation. Here, we describe a dual-mode multiplex immunoassay that integrates a lateral flow immunoassay (LFIA) with a magnetic bead immunoassay (MBIA) using spectrally resolved SiO2-encapsulated quantum dot (SiO2@QDs) probes. By combining SiO2@CdSe and carbon dot-based SiO2 nanoparticles, three spectrally distinct fluorescence signals (blue, green, and red) were generated under single-wavelength excitation (365 nm), enabling concurrent detection of anticyclic citrullinated peptide antibodies (anti-CCP), rheumatoid factor IgM (RF-IgM), and C-reactive protein (CRP). The LFIA module enables multiplex quantification within 10 min with minimal instrumentation, whereas the MBIA module extends analytical sensitivity and dynamic range through homogeneous immunoreactions and magnetic separation. The limits of detection of the MBIA sensor were 0.142 U/mL for anti-CCP, 0.057 IU/mL for RF-IgM, and 0.075 mg/L for CRP, with linear responses (R2 > 0.98), coefficients of variation below 8%, and minimal cross-reactivity. Analysis of clinical RA samples showed good agreement with commercial reference assays, as assessed by Passing–Bablok regression and Bland–Altman analysis. By integrating rapid screening and laboratory-grade quantification within a single fluorescence-encoded nanoprobe system, this dual-mode platform illustrates a modular strategy for multiplex autoimmune biomarker analysis, in which assay format and signal encoding are decoupled within a unified optical framework.

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Cite

BibTeXRIS
BibTeX
@article{yu2026singleexcitation,
  title = {{Single-Excitation, Orthogonally Encoded Quantum Dot Probes for Dual-Mode Multiplexed Biosensing}},
  author = {Heshan Yu and Yan Li and Rong Yang and Tingting Zhang and Shaoqing Zhang and Xudong He and Jing Ping and Yongjv Yang and Lijian Pang},
  journal = {ACS Omega},
  year = {2026},
  month = oct,
  doi = {10.1021/acsomega.6c07095},
  url = {https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c07095/5459286/Single-Excitation-Orthogonally-Encoded-Quantum-Dot},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - Single-Excitation, Orthogonally Encoded Quantum Dot Probes for Dual-Mode Multiplexed Biosensing
AU  - Heshan Yu
AU  - Yan Li
AU  - Rong Yang
AU  - Tingting Zhang
AU  - Shaoqing Zhang
AU  - Xudong He
AU  - Jing Ping
AU  - Yongjv Yang
AU  - Lijian Pang
PY  - 2026
DA  - 2026/10/27
JO  - ACS Omega
DO  - 10.1021/acsomega.6c07095
UR  - https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c07095/5459286/Single-Excitation-Orthogonally-Encoded-Quantum-Dot
AB  - Simultaneous quantitative detection of multiple rheumatoid arthritis (RA) biomarkers is often constrained by the need for separate analytical platforms and complex instrumentation. Here, we describe a dual-mode multiplex immunoassay that integrates a lateral flow immunoassay (LFIA) with a magnetic bead immunoassay (MBIA) using spectrally resolved SiO2-encapsulated quantum dot (SiO2@QDs) probes. By combining SiO2@CdSe and carbon dot-based SiO2 nanoparticles, three spectrally distinct fluorescence signals (blue, green, and red) were generated under single-wavelength excitation (365 nm), enabling concurrent detection of anticyclic citrullinated peptide antibodies (anti-CCP), rheumatoid factor IgM (RF-IgM), and C-reactive protein (CRP). The LFIA module enables multiplex quantification within 10 min with minimal instrumentation, whereas the MBIA module extends analytical sensitivity and dynamic range through homogeneous immunoreactions and magnetic separation. The limits of detection of the MBIA sensor were 0.142 U/mL for anti-CCP, 0.057 IU/mL for RF-IgM, and 0.075 mg/L for CRP, with linear responses (R2 > 0.98), coefficients of variation below 8%, and minimal cross-reactivity. Analysis of clinical RA samples showed good agreement with commercial reference assays, as assessed by Passing–Bablok regression and Bland–Altman analysis. By integrating rapid screening and laboratory-grade quantification within a single fluorescence-encoded nanoprobe system, this dual-mode platform illustrates a modular strategy for multiplex autoimmune biomarker analysis, in which assay format and signal encoding are decoupled within a unified optical framework.
SN  - 2470-1343
ER  -