Coal dust-stimulated macrophage-derived extracellular vesicles promote thyroid cancer cell phenotypes involving miR-205-5p, CAMK1G, and NF-κB-associated signaling
- 1The First Affiliated Hospital of Anhui University of Science and Technology
- 2School of Medicine, Anhui University of Science & Technology
- 3Anhui University of Science and Technology
Abstract
Background: Occupational coal dust exposure causes persistent pulmonary inflammation, but the mechanisms by which it may influence thyroid cancer progression remain unclear. Macrophage-derived extracellular vesicles (EVs) may participate in intercellular communication under particulate stress.
Objective: This study investigated whether EVs released by coal dust-stimulated macrophages alter thyroid cancer cell behavior and examined the involvement of miR-205-5p, CAMK1G, and NF-κB-associated signaling.
Methods: Coal dust was physicochemically characterized before use. EVs were isolated from PMA-differentiated THP-1 macrophages following coal dust exposure and characterized by transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. Small-RNA sequencing, bulk RNA sequencing, TCGA-THCA analysis, and the GSE274674 single-cell dataset were integrated with RT-qPCR, nuclear–cytoplasmic fractionation, dual-luciferase reporter assays, and genetic rescue experiments. Coal dust-exposed C57BL/6J mice and PBMC-humanized NSFG mice bearing TPC-1 xenografts were used for in vivo evaluation.
Results: PKH26-labeled EV-associated material was detected in TPC-1 cells, and EV preparations increased cell viability, clonogenic growth, migration, and invasion while reducing apoptosis. miR-205-5p was enriched in these EVs and accumulated predominantly in the nuclear fraction of recipient cells. Inhibition of miR-205-5p attenuated the EV-associated malignant phenotypes. Integrated transcriptomic analyses prioritized CAMK1G and NF-κB-related signaling. EV treatment increased endogenous CAMK1G expression and the phosphorylation of p65 and IκBα. CAMK1G knockdown reduced these changes, whereas CAMK1G re-expression partially restored the effects suppressed by miR-205-5p inhibition. The reporter assay showed sequence-dependent repression of the isolated CAMK1G 3′-UTR construct, whereas endogenous-expression and rescue experiments supported a positive functional association; therefore, these findings are not interpreted as a simple direct positive regulatory relationship. In mice, coal dust exposure was associated with pulmonary abnormalities, altered serum FT4 and TPO-Ab levels, increased thyroid CAMK1G expression, and enhanced pulmonary CD206 immunoreactivity. In PBMC-humanized mice, coal dust exposure or experimentally administered macrophage-derived EVs increased xenograft growth, whereas CAMK1G knockdown attenuated tumor burden.
Conclusion: These findings support a functional association between coal dust-stimulated macrophage-derived EV signaling and thyroid cancer progression involving miR-205-5p, CAMK1G, and NF-κB-associated activity. However, direct physiological trafficking of endogenous lung-derived EVs to thyroid or tumor tissue was not demonstrated.
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BibTeX
@article{zhao2026coal,
title = {{Coal dust-stimulated macrophage-derived extracellular vesicles promote thyroid cancer cell phenotypes involving miR-205-5p, CAMK1G, and NF-κB-associated signaling}},
author = {Feng Zhao and Tianqing Ma and Chi Zhang and Hongzhen Zhang},
journal = {Frontiers in Immunology},
year = {2026},
month = oct,
doi = {10.3389/fimmu.2026.1856770},
url = {https://www.frontiersin.org/articles/10.3389/fimmu.2026.1856770/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Coal dust-stimulated macrophage-derived extracellular vesicles promote thyroid cancer cell phenotypes involving miR-205-5p, CAMK1G, and NF-κB-associated signaling
AU - Feng Zhao
AU - Tianqing Ma
AU - Chi Zhang
AU - Hongzhen Zhang
PY - 2026
DA - 2026/10/14
JO - Frontiers in Immunology
DO - 10.3389/fimmu.2026.1856770
UR - https://www.frontiersin.org/articles/10.3389/fimmu.2026.1856770/full
AB - Background: Occupational coal dust exposure causes persistent pulmonary inflammation, but the mechanisms by which it may influence thyroid cancer progression remain unclear. Macrophage-derived extracellular vesicles (EVs) may participate in intercellular communication under particulate stress. Objective: This study investigated whether EVs released by coal dust-stimulated macrophages alter thyroid cancer cell behavior and examined the involvement of miR-205-5p, CAMK1G, and NF-κB-associated signaling. Methods: Coal dust was physicochemically characterized before use. EVs were isolated from PMA-differentiated THP-1 macrophages following coal dust exposure and characterized by transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. Small-RNA sequencing, bulk RNA sequencing, TCGA-THCA analysis, and the GSE274674 single-cell dataset were integrated with RT-qPCR, nuclear–cytoplasmic fractionation, dual-luciferase reporter assays, and genetic rescue experiments. Coal dust-exposed C57BL/6J mice and PBMC-humanized NSFG mice bearing TPC-1 xenografts were used for in vivo evaluation. Results: PKH26-labeled EV-associated material was detected in TPC-1 cells, and EV preparations increased cell viability, clonogenic growth, migration, and invasion while reducing apoptosis. miR-205-5p was enriched in these EVs and accumulated predominantly in the nuclear fraction of recipient cells. Inhibition of miR-205-5p attenuated the EV-associated malignant phenotypes. Integrated transcriptomic analyses prioritized CAMK1G and NF-κB-related signaling. EV treatment increased endogenous CAMK1G expression and the phosphorylation of p65 and IκBα. CAMK1G knockdown reduced these changes, whereas CAMK1G re-expression partially restored the effects suppressed by miR-205-5p inhibition. The reporter assay showed sequence-dependent repression of the isolated CAMK1G 3′-UTR construct, whereas endogenous-expression and rescue experiments supported a positive functional association; therefore, these findings are not interpreted as a simple direct positive regulatory relationship. In mice, coal dust exposure was associated with pulmonary abnormalities, altered serum FT4 and TPO-Ab levels, increased thyroid CAMK1G expression, and enhanced pulmonary CD206 immunoreactivity. In PBMC-humanized mice, coal dust exposure or experimentally administered macrophage-derived EVs increased xenograft growth, whereas CAMK1G knockdown attenuated tumor burden. Conclusion: These findings support a functional association between coal dust-stimulated macrophage-derived EV signaling and thyroid cancer progression involving miR-205-5p, CAMK1G, and NF-κB-associated activity. However, direct physiological trafficking of endogenous lung-derived EVs to thyroid or tumor tissue was not demonstrated.
SN - 1664-3224
ER -