Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 10 min ago
Peer-reviewedCC BYExtracellular vesicles and circulating nucleic acids

Dynamic profiles and subpopulation characteristics of extracellular vesicles in patients undergoing transcatheter pulmonary valve replacement.

  • Runzhang Liang1,2,3
  • Jiaxiong Wu1,2,3
  • Naijimuding Abudurexiti4,3
  • Jing Ling1,2,5
  • Zirui Peng2,5
  • Canxin Wang1,2
  • Jinxin Li1,2
  • Yong Zhang1,2,5
  • Haiyun Yuan1,2,5
  • Shusheng Wen1,2,5
5 affiliations
  1. 1Department of Cardiovascular Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, Guangdong, China.
  2. 2Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangzhou 510080, Guangdong, China.
  3. 3These authors contributed equally to this work.
  4. 4Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong, China.
  5. 5Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong, China.
Read full textPDFDOICite

Abstract

Aim: Transcatheter pulmonary valve replacement (TPVR) is a crucial intervention for severe pulmonary regurgitation (PR). Extracellular vesicle (EV) surface proteins reflect cardiovascular pathology. This study employed a proximity barcoding assay (PBA) to dynamically monitor EV protein profiles during TPVR. Methods: In this study, 40 biological plasma samples were analyzed in triplicate, including 10 from healthy controls (N group) and 30 from TPVR patients across three time points: preoperative (T1 group), 1 day postoperative (T2 group), and 1 week postoperative (T3 group). We performed combinatorial and functional analyses of membrane proteins across different groups and evaluated their predictive performance. EV subpopulations were clustered and characterized. Results: Compared with the N group, patients in the T1 group exhibited significantly downregulated expression of DSCAML1, ALCAM, and LAG3 proteins, while CD8A, NECTIN1, and LGR5 were significantly upregulated (P < 0.001; AUC: 0.94-1.00). Postoperatively, the expression of ALCAM and LAG3 rapidly rebounded. The ALCAM&CD36&DSCAML1&PECAM1 combination was significantly elevated in the control group, whereas CD36&CD8A&DSCAML1&TACSTD2 was reduced. Gene Ontology (GO) analysis indicated associations with response to stress, side of membrane, and protein binding pathways. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed enrichment in the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB/AKT) signaling pathway. Subpopulation analysis revealed that Cluster 1 was significantly elevated, whereas Cluster 2 was markedly decreased in the disease group. Additionally, Cluster 4 dominated in the T3 group. Furthermore, CD8A is most abundant in Cluster 1, while DSCAML1 dominates in Clusters 2 and 4. Conclusion: This study provides the first dynamic profiles and subpopulation characteristics of plasma EVs in patients undergoing TPVR, establishing a molecular atlas of perioperative right ventricular remodeling.

Related

  1. Extensive monolayer formation after transplantation depends on an RPE subpopulation derived from human iPSCs
    Karen Tessmer, Juliane Hammer, Sylvia J. Gasparini +20
    iSciencePeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationCell PressCrossrefEurope PMC
  2. Sustained photoprotection involves enhanced fluorescence intermittency in a subpopulation of LHCII
    Aurélie Crepin, Madeline P Hoffmann, Cristian Ilioaia +6
    HALPreprintCC BY 4.0Full textMolecular biology & gene regulationHAL
  3. Multi-gene biomarkers reveal spatial organization and subpopulation-specific damage response in biliary epithelial cells
    Kendall G. Kanakanui, Fransky Hantelys, Hannah R. Hrncir +2
    Development (Cambridge, England)Peer-reviewedCC BY 4.0Full text2 citedMolecular biology & gene regulationPMC
  4. Combined Nav1.7-Nav1.8 channel abrogation: Supralinear suppression of excitability in a subpopulation of peripheral sensory neurons.
    Dmytro Vasylyev, Sidharth Tyagi, Mohammad-Reza Ghovanloo +2
    The Journal of general physiologyPeer-reviewedCC BYFull textBiophysicsBioinformatics & computational biologyEurope PMCPMC
  5. Transcatheter Mitral Edge-to-edge Repair in Patients with Transthyretin Cardiac Amyloidosis: A Four-case Series
    Ayaka Ujita, Masayoshi Yamamoto, Tomoya Hoshi +5
    Internal MedicinePeer-reviewedCC BY-NC-NDFull text2 citedMolecular biology & gene regulationJ-STAGE

Cite

BibTeXRIS
BibTeX
@article{liang2026dynamic,
  title = {{Dynamic profiles and subpopulation characteristics of extracellular vesicles in patients undergoing transcatheter pulmonary valve replacement.}},
  author = {Runzhang Liang and Jiaxiong Wu and Naijimuding Abudurexiti and Jing Ling and Zirui Peng and Canxin Wang and Jinxin Li and Yong Zhang and Haiyun Yuan and Shusheng Wen},
  journal = {Extracellular vesicles and circulating nucleic acids},
  year = {2026},
  month = aug,
  doi = {10.20517/evcna.2026.24},
  url = {https://europepmc.org/article/MED/42898751},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Dynamic profiles and subpopulation characteristics of extracellular vesicles in patients undergoing transcatheter pulmonary valve replacement.
AU  - Runzhang Liang
AU  - Jiaxiong Wu
AU  - Naijimuding Abudurexiti
AU  - Jing Ling
AU  - Zirui Peng
AU  - Canxin Wang
AU  - Jinxin Li
AU  - Yong Zhang
AU  - Haiyun Yuan
AU  - Shusheng Wen
PY  - 2026
DA  - 2026/08/18
JO  - Extracellular vesicles and circulating nucleic acids
DO  - 10.20517/evcna.2026.24
UR  - https://europepmc.org/article/MED/42898751
AB  - Aim: Transcatheter pulmonary valve replacement (TPVR) is a crucial intervention for severe pulmonary regurgitation (PR). Extracellular vesicle (EV) surface proteins reflect cardiovascular pathology. This study employed a proximity barcoding assay (PBA) to dynamically monitor EV protein profiles during TPVR. Methods: In this study, 40 biological plasma samples were analyzed in triplicate, including 10 from healthy controls (N group) and 30 from TPVR patients across three time points: preoperative (T1 group), 1 day postoperative (T2 group), and 1 week postoperative (T3 group). We performed combinatorial and functional analyses of membrane proteins across different groups and evaluated their predictive performance. EV subpopulations were clustered and characterized. Results: Compared with the N group, patients in the T1 group exhibited significantly downregulated expression of DSCAML1, ALCAM, and LAG3 proteins, while CD8A, NECTIN1, and LGR5 were significantly upregulated (P < 0.001; AUC: 0.94-1.00). Postoperatively, the expression of ALCAM and LAG3 rapidly rebounded. The ALCAM&CD36&DSCAML1&PECAM1 combination was significantly elevated in the control group, whereas CD36&CD8A&DSCAML1&TACSTD2 was reduced. Gene Ontology (GO) analysis indicated associations with response to stress, side of membrane, and protein binding pathways. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed enrichment in the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB/AKT) signaling pathway. Subpopulation analysis revealed that Cluster 1 was significantly elevated, whereas Cluster 2 was markedly decreased in the disease group. Additionally, Cluster 4 dominated in the T3 group. Furthermore, CD8A is most abundant in Cluster 1, while DSCAML1 dominates in Clusters 2 and 4. Conclusion: This study provides the first dynamic profiles and subpopulation characteristics of plasma EVs in patients undergoing TPVR, establishing a molecular atlas of perioperative right ventricular remodeling.
SN  - 2767-6641
ER  -