Equine (Equus caballus) primary post-natal brain-derived cells: immortalization and preliminary reprogramming attempts to induced pluripotent stem cells.
- 1Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
- 2Department of Molecular Medicine, University of Padua, Padua, Italy.
- 3Department of Agronomy, Food, Natural Resources, Animals, and Environment, University of Padua, Padua, Italy.
Abstract
Equines represent an underexplored yet highly relevant model for studying neurological diseases within a One Health framework. Here, we report the first establishment of post-natal equine brain-derived in vitro systems, including equine primary post-natal brain-derived cells (EpBCs) and their immortalized counterparts (EiBCs). EpBCs were isolated from the hippocampus and successfully expanded, while immortalization via Simian virus 40 large T antigen generated EiBCs lines with distinct transcriptomic reprogramming characterized by cell cycle activation and partial dedifferentiation. Despite testing three non-integrating approaches-Sendai virus, episomal vectors, and synthetic mRNA-no induced pluripotent stem cells (iPSCs)-like colonies were detected under the tested conditions, possibly reflecting a combination of species-specific, cell-intrinsic, and donor-specific factors, as well as culture conditions, factor compatibility, and delivery efficiency. Together, these findings establish a foundational equine brain-derived cell platform and highlight key challenges in equine reprogramming. Its One Health relevance is prospective, as this system may support future comparative neurological research and the development of equine brain organoids.
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BibTeX
@article{molinari2026equine,
title = {{Equine (Equus caballus) primary post-natal brain-derived cells: immortalization and preliminary reprogramming attempts to induced pluripotent stem cells.}},
author = {Antonella Molinari and Camilla Lucca and Stefania Vogiatzis and Valentina Moccia and Massimiliano Babbucci and Luca Peruzza and Enrico Negrisolo and Marta Trevisan and Luisa Barzon and Cinzia Centelleghe and Sandro Mazzariol and Valentina Elena Giuditta Zappulli},
journal = {Frontiers in veterinary science},
year = {2026},
month = oct,
doi = {10.3389/fvets.2026.1931938},
url = {https://europepmc.org/article/MED/42909034},
note = {CC BY},
}
RIS
TY - JOUR
TI - Equine (Equus caballus) primary post-natal brain-derived cells: immortalization and preliminary reprogramming attempts to induced pluripotent stem cells.
AU - Antonella Molinari
AU - Camilla Lucca
AU - Stefania Vogiatzis
AU - Valentina Moccia
AU - Massimiliano Babbucci
AU - Luca Peruzza
AU - Enrico Negrisolo
AU - Marta Trevisan
AU - Luisa Barzon
AU - Cinzia Centelleghe
AU - Sandro Mazzariol
AU - Valentina Elena Giuditta Zappulli
PY - 2026
DA - 2026/10/07
JO - Frontiers in veterinary science
DO - 10.3389/fvets.2026.1931938
UR - https://europepmc.org/article/MED/42909034
AB - Equines represent an underexplored yet highly relevant model for studying neurological diseases within a One Health framework. Here, we report the first establishment of post-natal equine brain-derived in vitro systems, including equine primary post-natal brain-derived cells (EpBCs) and their immortalized counterparts (EiBCs). EpBCs were isolated from the hippocampus and successfully expanded, while immortalization via Simian virus 40 large T antigen generated EiBCs lines with distinct transcriptomic reprogramming characterized by cell cycle activation and partial dedifferentiation. Despite testing three non-integrating approaches-Sendai virus, episomal vectors, and synthetic mRNA-no induced pluripotent stem cells (iPSCs)-like colonies were detected under the tested conditions, possibly reflecting a combination of species-specific, cell-intrinsic, and donor-specific factors, as well as culture conditions, factor compatibility, and delivery efficiency. Together, these findings establish a foundational equine brain-derived cell platform and highlight key challenges in equine reprogramming. Its One Health relevance is prospective, as this system may support future comparative neurological research and the development of equine brain organoids.
SN - 2297-1769
ER -