A Colorimetric Strategy for Arsenic(V) Determination in Rice via Boosting the Peroxidase-like Activity of NCuzyme
- 1School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China
Abstract
Arsenic (As) is extremely poisonous, and prolonged exposure greatly raises the likelihood of developing cancer. It is crucial to develop selective and sensitive methods for detecting As. In this study, using melamine and Cu(II), a Cu-based complex (NCuzyme) with peroxidase (POD)-like activity was prepared using a green and simple approach for the first time. NCuzyme can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) with H2O2 to form a blue product (oxTMB). The addition of As(V) can boost the POD-like activity of NCuzyme, deepening the blue color and increasing the absorbance signal of the reaction system. Therefore, a colorimetric method has been developed for the detection of As(V). This method exhibits excellent selectivity for As(V), with a linear range of 4–150 μg/L and a limit of detection of 3.35 μg/L, which is below the limit for arsenic in drinking water (10 μg/L) set by the World Health Organization. Finally, the developed colorimetric sensing method has been successfully applied to detect As(V) in real sample (rice), with good recovery rates (93.3–102.9%) and an RSD of less than 2.3%. In summary, the colorimetric method developed based on enhancing POD-like activity of NCuzyme provides a rapid and simple method for the As(V) detection.
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BibTeX
@article{xu2026colorimetric,
title = {{A Colorimetric Strategy for Arsenic(V) Determination in Rice via Boosting the Peroxidase-like Activity of NCuzyme}},
author = {Lei Xu and Mao-Ling Luo and Shan Qiu and Jing-Jing Dai and Huan Zhu and Feng-Qing Yang},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31193494},
url = {https://www.mdpi.com/1420-3049/31/19/3494},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - A Colorimetric Strategy for Arsenic(V) Determination in Rice via Boosting the Peroxidase-like Activity of NCuzyme
AU - Lei Xu
AU - Mao-Ling Luo
AU - Shan Qiu
AU - Jing-Jing Dai
AU - Huan Zhu
AU - Feng-Qing Yang
PY - 2026
DA - 2026/10/01
JO - Molecules
DO - 10.3390/molecules31193494
UR - https://www.mdpi.com/1420-3049/31/19/3494
AB - Arsenic (As) is extremely poisonous, and prolonged exposure greatly raises the likelihood of developing cancer. It is crucial to develop selective and sensitive methods for detecting As. In this study, using melamine and Cu(II), a Cu-based complex (NCuzyme) with peroxidase (POD)-like activity was prepared using a green and simple approach for the first time. NCuzyme can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) with H2O2 to form a blue product (oxTMB). The addition of As(V) can boost the POD-like activity of NCuzyme, deepening the blue color and increasing the absorbance signal of the reaction system. Therefore, a colorimetric method has been developed for the detection of As(V). This method exhibits excellent selectivity for As(V), with a linear range of 4–150 μg/L and a limit of detection of 3.35 μg/L, which is below the limit for arsenic in drinking water (10 μg/L) set by the World Health Organization. Finally, the developed colorimetric sensing method has been successfully applied to detect As(V) in real sample (rice), with good recovery rates (93.3–102.9%) and an RSD of less than 2.3%. In summary, the colorimetric method developed based on enhancing POD-like activity of NCuzyme provides a rapid and simple method for the As(V) detection.
SN - 1420-3049
ER -