Optimization of Supercritical CO2 Extraction Conditions and Metabolite Composition of Chamaepericlymenum suecicum L. Berries from Two Locations in the Magadan Region
5 affiliations
- 1N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, Saint-Petersburg 190000, Russia
- 2Advanced Engineering School, Institute of Biotechnology, Bioengineering and Food Systems, Far Eastern Federal University, Fr. Russian, pos. Ajax, 10, Vladivostok 690922, Russia
- 3Higher Engineering School of Agrobiotechnology, National Research Tomsk State University, Lenin Ave, 36, Tomsk 634050, Russia
- 4Laboratory for Research and Application of Supercritical Fluid Technologies in Agro-Food Biotechnology, National Research Tomsk State University, Lenin Ave, 36, Tomsk 634050, Russia
- 5Siberian Federal Scientific Centre of Agrobiotechnology RAS, 7 Centralnaya Street, Krasnoobsk 633501, Russia
Abstract
Supercritical fluid extraction (SFE) using CO2 has many advantages for recovering bioactive compounds, such as high selectivity, minimal solvent use and preservation of thermolabile metabolites. This study optimized SFE for extracting Chamaepericlymenum suecicum (Eurasian dwarf cornel) berries and compared the metabolite profiles of samples from two locations in the Magadan region: Magadan and Ola. The extraction process was conducted at temperatures ranging from 100 to 400 °C and pressures ranging from 31 to 70 MPa, using 1% ethanol as a co-solvent. Global yield ranged from 2.60 to 8.45 mg/100 mg, with the highest yield (8.45 mg/100 mg) obtained at 250 bar and 55 °C. Two-way ANOVA revealed that temperature, pressure, and their interaction significantly influenced the extraction yield (p < 0.001). A total of 47 compounds were identified by HPLC-ion trap-MS/MS, including 33 polyphenolic compounds (flavones, flavonols, flavan-3-ols, anthocyanins, phenolic acids, coumarins, and lignans), as well as 14 other metabolites (including fatty acids, oxylipins, alkaloids, organic acids, sugars, naphthoquinones, and anabolic steroids). The metabolite profiles differed between the two locations: Magadan samples contained 26 unique polyphenolic compounds, while Ola samples were characterized by the presence of flavan-3-ols, which were absent in Magadan samples. Only nine compounds were shared between the two locations. The number of compounds ranged from 8 to 24, and the Shannon diversity index was higher in the Magadan samples (H′ = 3.178 and 2.303) than in the Ola samples (H′ = 2.079 and 2.398). These findings demonstrate that supercritical fluid extraction (SFE) combined with metabolomic analysis is a powerful tool for profiling C. suecicum berries, revealing significant geographic variation in their chemical composition and highlighting their potential as a source of bioactive compounds for functional foods and pharmaceutical applications.
Related
- SFEModeling.jl: multi-model, multi-curve fitting of supercritical fluid extraction kinetics with a graphical interface
- Supercritical CO₂ extraction of onion seed oil and its synergistic effect with gentamicin against bacterial strains.
- Winery By-Products as Sustainable Functional Resources: An Overview of Composition, Green Extraction Technologies, and Industrial Valorization
- Effect of extraction temperature on the fatty acid composition, metabolic profile, and bioactivity-related properties of Lepidium sativum seed oil
- Pore Solution of Stoichiometric MKP Cement Paste: Study of Composition Using Different Extraction Protocols and Thermodynamic Modelling
BibTeX
@article{razgonova2026optimization,
title = {{Optimization of Supercritical CO2 Extraction Conditions and Metabolite Composition of Chamaepericlymenum suecicum L. Berries from Two Locations in the Magadan Region}},
author = {Mayya P. Razgonova and Muhammad A. Nawaz and Andrey S. Sabitov and Tamara A. Senotrusova and Natalya G. Li and Kirill S. Golokhvast},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31203658},
url = {https://www.mdpi.com/1420-3049/31/20/3658},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Optimization of Supercritical CO2 Extraction Conditions and Metabolite Composition of Chamaepericlymenum suecicum L. Berries from Two Locations in the Magadan Region
AU - Mayya P. Razgonova
AU - Muhammad A. Nawaz
AU - Andrey S. Sabitov
AU - Tamara A. Senotrusova
AU - Natalya G. Li
AU - Kirill S. Golokhvast
PY - 2026
DA - 2026/10/01
JO - Molecules
DO - 10.3390/molecules31203658
UR - https://www.mdpi.com/1420-3049/31/20/3658
AB - Supercritical fluid extraction (SFE) using CO2 has many advantages for recovering bioactive compounds, such as high selectivity, minimal solvent use and preservation of thermolabile metabolites. This study optimized SFE for extracting Chamaepericlymenum suecicum (Eurasian dwarf cornel) berries and compared the metabolite profiles of samples from two locations in the Magadan region: Magadan and Ola. The extraction process was conducted at temperatures ranging from 100 to 400 °C and pressures ranging from 31 to 70 MPa, using 1% ethanol as a co-solvent. Global yield ranged from 2.60 to 8.45 mg/100 mg, with the highest yield (8.45 mg/100 mg) obtained at 250 bar and 55 °C. Two-way ANOVA revealed that temperature, pressure, and their interaction significantly influenced the extraction yield (p < 0.001). A total of 47 compounds were identified by HPLC-ion trap-MS/MS, including 33 polyphenolic compounds (flavones, flavonols, flavan-3-ols, anthocyanins, phenolic acids, coumarins, and lignans), as well as 14 other metabolites (including fatty acids, oxylipins, alkaloids, organic acids, sugars, naphthoquinones, and anabolic steroids). The metabolite profiles differed between the two locations: Magadan samples contained 26 unique polyphenolic compounds, while Ola samples were characterized by the presence of flavan-3-ols, which were absent in Magadan samples. Only nine compounds were shared between the two locations. The number of compounds ranged from 8 to 24, and the Shannon diversity index was higher in the Magadan samples (H′ = 3.178 and 2.303) than in the Ola samples (H′ = 2.079 and 2.398). These findings demonstrate that supercritical fluid extraction (SFE) combined with metabolomic analysis is a powerful tool for profiling C. suecicum berries, revealing significant geographic variation in their chemical composition and highlighting their potential as a source of bioactive compounds for functional foods and pharmaceutical applications.
SN - 1420-3049
ER -