Characterization of Rosa damascena Callus-Derived Exosome-like Vesicles and Their Multifunctional Activities in Skin-Related Cellular Models
- 1Department of Prescriptionology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; [email protected] (J.J.L.); [email protected] (J.Y.H.); [email protected] (S.K.P.); [email protected] (C.P.); [email protected] (M.S.C.)
- 2ExoCoBio Exosome Institute (EEI), ExoCoBio Inc., Seoul 08594, Republic of Korea; [email protected] (B.S.C.); [email protected] (H.J.L.); [email protected] (B.S.); [email protected] (E.L.); [email protected] (S.Y.M.); [email protected] (E.S.); [email protected] (D.H.H.)
- 3Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea; Natural Science Institute, Hanyang University, Seoul 04763, Republic of Korea; Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea; [email protected] (K.L.)
Abstract
callus culture medium (RSC-EXO) and evaluated their molecular features and biological activities in skin-related cellular models. Nanoparticle tracking analysis and cryo-electron microscopy showed that RSC-EXO exhibited a nanoscale size distribution and spherical morphology. Western blotting confirmed enrichment of the plant EV-associated markers PEN1 and TET8. RSC-EXO were efficiently internalized by human dermal fibroblasts and showed markedly improved biocompatibility compared with crude conditioned medium (RSC-CM). Functionally, RSC-EXO significantly increased collagen synthesis and showed a trend toward enhanced wound closure in fibroblasts. In addition, RSC-EXO reduced melanin production in α-MSH-stimulated B16F10 melanoma cells and suppressed the secretion of pro-inflammatory cytokines, including IL-1α, IL-6, and TNF-α, in LPS-stimulated RAW 264.7 macrophages. Proteomic analysis revealed a distinct cargo enriched in stress-, defense-, and metabolism-related proteins, while small RNA sequencing identified a heterogeneous small RNA population containing a limited fraction of miRNA-sized reads. Collectively, these findings suggest that RSC-EXO represents a biologically active plant-derived vesicle population with regenerative and anti-inflammatory activity observed in vitro in skin-related cellular models and support its potential as a promising platform for future cosmeceutical and dermatological applications.
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BibTeX
@article{cho2026characterization,
title = {{Characterization of Rosa damascena Callus-Derived Exosome-like Vesicles and Their Multifunctional Activities in Skin-Related Cellular Models}},
author = {Byong Seung Cho and Hyun Jung Lee and Bogeun Son and Esther Lee and Sang Yun Moon and Ella Shin and Jeong Jin Lee and Jun Young Hur and Seong Kyu Park and Cholhyun Park and Kyung-Min Lee and Dae Hyun Ha and Mun Seog Chang},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = may,
doi = {10.3390/ijms27114938},
url = {https://doi.org/10.3390/ijms27114938},
note = {CC BY},
}
RIS
TY - JOUR
TI - Characterization of Rosa damascena Callus-Derived Exosome-like Vesicles and Their Multifunctional Activities in Skin-Related Cellular Models
AU - Byong Seung Cho
AU - Hyun Jung Lee
AU - Bogeun Son
AU - Esther Lee
AU - Sang Yun Moon
AU - Ella Shin
AU - Jeong Jin Lee
AU - Jun Young Hur
AU - Seong Kyu Park
AU - Cholhyun Park
AU - Kyung-Min Lee
AU - Dae Hyun Ha
AU - Mun Seog Chang
PY - 2026
DA - 2026/05/29
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27114938
UR - https://doi.org/10.3390/ijms27114938
AB - callus culture medium (RSC-EXO) and evaluated their molecular features and biological activities in skin-related cellular models. Nanoparticle tracking analysis and cryo-electron microscopy showed that RSC-EXO exhibited a nanoscale size distribution and spherical morphology. Western blotting confirmed enrichment of the plant EV-associated markers PEN1 and TET8. RSC-EXO were efficiently internalized by human dermal fibroblasts and showed markedly improved biocompatibility compared with crude conditioned medium (RSC-CM). Functionally, RSC-EXO significantly increased collagen synthesis and showed a trend toward enhanced wound closure in fibroblasts. In addition, RSC-EXO reduced melanin production in α-MSH-stimulated B16F10 melanoma cells and suppressed the secretion of pro-inflammatory cytokines, including IL-1α, IL-6, and TNF-α, in LPS-stimulated RAW 264.7 macrophages. Proteomic analysis revealed a distinct cargo enriched in stress-, defense-, and metabolism-related proteins, while small RNA sequencing identified a heterogeneous small RNA population containing a limited fraction of miRNA-sized reads. Collectively, these findings suggest that RSC-EXO represents a biologically active plant-derived vesicle population with regenerative and anti-inflammatory activity observed in vitro in skin-related cellular models and support its potential as a promising platform for future cosmeceutical and dermatological applications.
SN - 1422-0067
SN - 1661-6596
ER -