Quercus salicina Blume Inhibits Bone Loss via Dual Effects on Osteoblasts and Osteoclasts
4 affiliations
- 1Gangneung Science & Industrial Promotion Agency, Gangneung 25440, Republic of Korea
- 2Smart Farm Research Center, Gangneung Institute of Natural Products, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of Korea
- 3Division of Natural Product Applied Science, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of Korea
- 4Department of Plant Science, Kangwon National University, Gangneung 25457, Republic of Korea
Abstract
Background/Objectives: Osteoporosis is a progressive metabolic bone disorder with reduced bone mass and microarchitectural deterioration, leading to an increased risk of fractures. With an aging population, there is a growing demand for safe and effective long-term therapeutic methods for osteoporosis and medicinal plants could be promising candidates as novel agents for osteoporosis. In this study, we investigated the effects of the 70% ethanol extract of Quercus salicina Blume (QS), an herbal plant, on osteoporosis. Methods: MC3T3-E1 mouse pre-osteoblast cells and RAW264.7 mouse macrophage cells were utilized to assess osteoblast and osteoclast differentiation, respectively. QSEE (10 mg/kg and 40 mg/kg) was orally administered for 12 weeks in OVX mice, a postmenopausal osteoporosis animal model, and bone parameters were evaluated using Micro-Computed Tomography (Micro-CT). Ex vivo experiments were conducted using primary cells isolated from mouse femurs and tibias. Expression levels of proteins and mRNAs related to osteoblasts and osteoclasts were estimated by Western blot and Quantitative Real-Time PCR (qRT-PCR). Results: QSEE showed osteogenic activity by increasing alkaline phosphatase (ALP) activity, protein and mRNA expression related to osteoblast differentiation. QSEE decreased osteoclast differentiation by suppressing tartrate-resistant acid phosphatase (TRAP) activity, protein and mRNA expression related to osteoclast differentiation. In OVX mice, QSEE administration restored bone microarchitecture decreased by OVX. In primary cells isolated from mouse femurs, QSEE increased osteoblast-related markers and decreased osteoclast-related markers. Conclusions: These findings suggest Quercus salicina Blume has potential as an anti-osteoporosis agent by inducing osteoblast differentiation and suppressing osteoclast differentiation.
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BibTeX
@article{cho2026quercus,
title = {{Quercus salicina Blume Inhibits Bone Loss via Dual Effects on Osteoblasts and Osteoclasts}},
author = {Suhyun Cho and Nadzeya Lazerka and Soyeon Hong and Minji Gwon and Byeong Jun Jeon and Jeong Do Kim and Gyhye Yoo},
journal = {Nutrients},
year = {2026},
month = oct,
doi = {10.3390/nu18213578},
url = {https://www.mdpi.com/2072-6643/18/21/3578},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Quercus salicina Blume Inhibits Bone Loss via Dual Effects on Osteoblasts and Osteoclasts
AU - Suhyun Cho
AU - Nadzeya Lazerka
AU - Soyeon Hong
AU - Minji Gwon
AU - Byeong Jun Jeon
AU - Jeong Do Kim
AU - Gyhye Yoo
PY - 2026
DA - 2026/10/29
JO - Nutrients
DO - 10.3390/nu18213578
UR - https://www.mdpi.com/2072-6643/18/21/3578
AB - Background/Objectives: Osteoporosis is a progressive metabolic bone disorder with reduced bone mass and microarchitectural deterioration, leading to an increased risk of fractures. With an aging population, there is a growing demand for safe and effective long-term therapeutic methods for osteoporosis and medicinal plants could be promising candidates as novel agents for osteoporosis. In this study, we investigated the effects of the 70% ethanol extract of Quercus salicina Blume (QS), an herbal plant, on osteoporosis. Methods: MC3T3-E1 mouse pre-osteoblast cells and RAW264.7 mouse macrophage cells were utilized to assess osteoblast and osteoclast differentiation, respectively. QSEE (10 mg/kg and 40 mg/kg) was orally administered for 12 weeks in OVX mice, a postmenopausal osteoporosis animal model, and bone parameters were evaluated using Micro-Computed Tomography (Micro-CT). Ex vivo experiments were conducted using primary cells isolated from mouse femurs and tibias. Expression levels of proteins and mRNAs related to osteoblasts and osteoclasts were estimated by Western blot and Quantitative Real-Time PCR (qRT-PCR). Results: QSEE showed osteogenic activity by increasing alkaline phosphatase (ALP) activity, protein and mRNA expression related to osteoblast differentiation. QSEE decreased osteoclast differentiation by suppressing tartrate-resistant acid phosphatase (TRAP) activity, protein and mRNA expression related to osteoclast differentiation. In OVX mice, QSEE administration restored bone microarchitecture decreased by OVX. In primary cells isolated from mouse femurs, QSEE increased osteoblast-related markers and decreased osteoclast-related markers. Conclusions: These findings suggest Quercus salicina Blume has potential as an anti-osteoporosis agent by inducing osteoblast differentiation and suppressing osteoclast differentiation.
SN - 2072-6643
ER -