Isolation, structural characterization of oligosaccharide AMO-1 from Avicennia marina fruit and its anti-osteoporotic effects via PI3K/AKT/HIF‑1α signaling pathway activation.
8 affiliations
- 1The Marine Biomedical Research Institute of Guangdong Zhanjiang, School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, 524023, People's Republic of China.
- 2Department of Environmental Science and Engineering, Fudan University, Shanghai, 200438, People's Republic of China.
- 3The Marine Biomedical Research Institute of Guangdong Zhanjiang, School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, 524023, People's Republic of China. [email protected].
- 4Guangdong Engineering Technology Research Center for the Development and Utilization of Mangrove Wetland Medicinal Resources, Guangdong Medical University, Zhanjiang, 524023, People's Republic of China. [email protected].
- 5College of Tropical Southern Medicinal Herbs and Marine Organism Utilization, Guangdong Medical University, Zhanjiang, 524023, People's Republic of China. [email protected].
- 6The Marine Biomedical Research Institute of Guangdong Zhanjiang, School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, 524023, People's Republic of China. [email protected].
- 7Guangdong Engineering Technology Research Center for the Development and Utilization of Mangrove Wetland Medicinal Resources, Guangdong Medical University, Zhanjiang, 524023, People's Republic of China. [email protected].
- 8College of Tropical Southern Medicinal Herbs and Marine Organism Utilization, Guangdong Medical University, Zhanjiang, 524023, People's Republic of China. [email protected].
Abstract
Avicennia marina (Forssk.) Vierh. is an important mangrove plant with high nutritional and medicinal values, featuring saccharides as its primary bioactive compounds. In this study, we successfully isolated a neutral tetrasaccharide (AMO-1) from the fruits of A. marina. Through monosaccharide composition, methylation, UPLC-ESI-MS/MS, and NMR analyses, we revealed that the structure of AMO-1 was α-D-Galp-(1 → 6)-α-D-Galp-(1 → 6)-α-D-Glcp-(1 → 2)-β-D-Fruf. Subsequently, we investigated the anti-osteoporosis (anti-OP) efficacy and underlying mechanisms of AMO-1. Our findings demonstrated that AMO-1 promoted the differentiation and mineralization of MC3T3-E1 cells. Furthermore, AMO-1 increased trabecular bone density and restored its reticular structure in dexamethasone-induced OP mice. Additionally, AMO-1 upregulated the expression levels of osteogenic factors and elevated serum levels of osteocalcin and PINP. Mechanistically, AMO-1 promoted osteogenesis through activation of the PI3K/AKT/HIF-1α signaling pathway, thus exerting anti-OP effects. Overall, these findings indicate that AMO-1 is a promising functional food ingredient with therapeutic potential for the prevention and management of OP.
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BibTeX
@article{zhou2026isolation,
title = {{Isolation, structural characterization of oligosaccharide AMO-1 from Avicennia marina fruit and its anti-osteoporotic effects via PI3K/AKT/HIF‑1α signaling pathway activation.}},
author = {Lishuang Zhou and Zhenhui Feng and Yijing Zhang and Lei Tan and Pan Zhou and Xia Wu and Xing Chen and Qinyuan Zhang and Qiwen Cai and Liutong Yu and Kefeng Wu and Luming Deng},
journal = {Natural products and bioprospecting},
year = {2026},
month = oct,
doi = {10.1007/s13659-026-00646-8},
url = {https://europepmc.org/article/MED/42911466},
note = {CC BY},
}
RIS
TY - JOUR
TI - Isolation, structural characterization of oligosaccharide AMO-1 from Avicennia marina fruit and its anti-osteoporotic effects via PI3K/AKT/HIF‑1α signaling pathway activation.
AU - Lishuang Zhou
AU - Zhenhui Feng
AU - Yijing Zhang
AU - Lei Tan
AU - Pan Zhou
AU - Xia Wu
AU - Xing Chen
AU - Qinyuan Zhang
AU - Qiwen Cai
AU - Liutong Yu
AU - Kefeng Wu
AU - Luming Deng
PY - 2026
DA - 2026/10/22
JO - Natural products and bioprospecting
DO - 10.1007/s13659-026-00646-8
UR - https://europepmc.org/article/MED/42911466
AB - Avicennia marina (Forssk.) Vierh. is an important mangrove plant with high nutritional and medicinal values, featuring saccharides as its primary bioactive compounds. In this study, we successfully isolated a neutral tetrasaccharide (AMO-1) from the fruits of A. marina. Through monosaccharide composition, methylation, UPLC-ESI-MS/MS, and NMR analyses, we revealed that the structure of AMO-1 was α-D-Galp-(1 → 6)-α-D-Galp-(1 → 6)-α-D-Glcp-(1 → 2)-β-D-Fruf. Subsequently, we investigated the anti-osteoporosis (anti-OP) efficacy and underlying mechanisms of AMO-1. Our findings demonstrated that AMO-1 promoted the differentiation and mineralization of MC3T3-E1 cells. Furthermore, AMO-1 increased trabecular bone density and restored its reticular structure in dexamethasone-induced OP mice. Additionally, AMO-1 upregulated the expression levels of osteogenic factors and elevated serum levels of osteocalcin and PINP. Mechanistically, AMO-1 promoted osteogenesis through activation of the PI3K/AKT/HIF-1α signaling pathway, thus exerting anti-OP effects. Overall, these findings indicate that AMO-1 is a promising functional food ingredient with therapeutic potential for the prevention and management of OP.
SN - 2192-2195
SN - 2192-2209
ER -