Isolation and characterization of two new cytotoxic glycosylsphingolipids from the stem bark of Pithecellobium eriorhachis Harms. (Fabaceae)
Abstract
Pithecellobium eriorhachis Harms . (Fabaceae) has been used as a folk medicine to treat various diseases in Cameroon and other countries. The present study aimed to isolate and characterise compounds with cytotoxic activity against three glioblastoma cancer cell lines (U87-MG, U251-MG, LN229) from the stem bark of P. eriorachis . As a result, two new cerebrosides, trivially named Pithecellobioside A and B ( 1–2 ), together with seven known compounds, were isolated by silica gel column chromatography, including one alkaloid ( 3 ), four flavonoids ( 4–7 ) and two steroids ( 8–9 ). The structures of all isolated compounds were elucidated using extensive spectral data, including 1D and 2D NMR, HR-ESI-MS, and comparison of their NMR data with those of previously reported compounds. Both new compounds ( 1 – 2 ) were assessed in vitro using the MTT assay on three glioblastoma cancer cell lines (U87-MG, U251-MG, and LN229). U87-MG cells were the most sensitive to compound 1 (IC 50 = 1.23 ± 0.06 μg/mL), while compound 2 showed the highest activity against LN229 cells (IC 50 = 2.68 ± 0.08 μg/mL), exceeding that of the reference drug (oxaliplatin against LN229, IC 50 = 4.78 ± 0.56 μg/mL). Pithecellobioside B ( 2 ) is identified as a potent cytotoxic compound that warrants further investigation for the development of novel antiproliferative drugs targeting LN229 epithelial-like glioblastoma cells. These findings indicate that Pithecellobiosides A and B ( 1–2 ) represent new cerebrosides with selective cytotoxicity against glioblastoma cells, highlighting P. eriorachis as a promising source of bioactive glycosphingolipids.
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BibTeX
@article{melong2026isolation,
title = {{Isolation and characterization of two new cytotoxic glycosylsphingolipids from the stem bark of Pithecellobium eriorhachis Harms. (Fabaceae)}},
author = {Raduis Melong and Steven Collins Njonte Wouamba and Armelle Tsamo Tontsa and Jean Paul Dzoyem and Éric Allémann and Florence Delie and Michel Gelbcke and Franck Meyer and Gilbert Deccaux Wabo Fotso Kapche},
journal = {Fitoterapia},
year = {2026},
month = oct,
doi = {10.1016/j.fitote.2026.107248},
url = {https://hal.science/hal-05790557v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - Isolation and characterization of two new cytotoxic glycosylsphingolipids from the stem bark of Pithecellobium eriorhachis Harms. (Fabaceae)
AU - Raduis Melong
AU - Steven Collins Njonte Wouamba
AU - Armelle Tsamo Tontsa
AU - Jean Paul Dzoyem
AU - Éric Allémann
AU - Florence Delie
AU - Michel Gelbcke
AU - Franck Meyer
AU - Gilbert Deccaux Wabo Fotso Kapche
PY - 2026
DA - 2026/10/10
JO - Fitoterapia
DO - 10.1016/j.fitote.2026.107248
UR - https://hal.science/hal-05790557v1
AB - Pithecellobium eriorhachis Harms . (Fabaceae) has been used as a folk medicine to treat various diseases in Cameroon and other countries. The present study aimed to isolate and characterise compounds with cytotoxic activity against three glioblastoma cancer cell lines (U87-MG, U251-MG, LN229) from the stem bark of P. eriorachis . As a result, two new cerebrosides, trivially named Pithecellobioside A and B ( 1–2 ), together with seven known compounds, were isolated by silica gel column chromatography, including one alkaloid ( 3 ), four flavonoids ( 4–7 ) and two steroids ( 8–9 ). The structures of all isolated compounds were elucidated using extensive spectral data, including 1D and 2D NMR, HR-ESI-MS, and comparison of their NMR data with those of previously reported compounds. Both new compounds ( 1 – 2 ) were assessed in vitro using the MTT assay on three glioblastoma cancer cell lines (U87-MG, U251-MG, and LN229). U87-MG cells were the most sensitive to compound 1 (IC 50 = 1.23 ± 0.06 μg/mL), while compound 2 showed the highest activity against LN229 cells (IC 50 = 2.68 ± 0.08 μg/mL), exceeding that of the reference drug (oxaliplatin against LN229, IC 50 = 4.78 ± 0.56 μg/mL). Pithecellobioside B ( 2 ) is identified as a potent cytotoxic compound that warrants further investigation for the development of novel antiproliferative drugs targeting LN229 epithelial-like glioblastoma cells. These findings indicate that Pithecellobiosides A and B ( 1–2 ) represent new cerebrosides with selective cytotoxicity against glioblastoma cells, highlighting P. eriorachis as a promising source of bioactive glycosphingolipids.
SN - 0367-326X
SN - 1873-6971
ER -