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Peer-reviewedCC BY-NC-ND 4.0STAR Protocols

A live-imaging protocol to track cell movement in the Xenopus embryo

  • Alexandre Chuyen
  • Fabrice Daian
  • Andrea Pasini
  • Laurent Kodjabachian
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Abstract

A live-imaging protocol to track cell movement in the Xenopus embryo Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded.

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Cite

BibTeXRIS
BibTeX
@article{chuyen2026liveimaging,
  title = {{A live-imaging protocol to track cell movement in the Xenopus embryo}},
  author = {Alexandre Chuyen and Fabrice Daian and Andrea Pasini and Laurent Kodjabachian},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2021.100928},
  url = {https://hal.science/hal-05790503v1},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - A live-imaging protocol to track cell movement in the Xenopus embryo
AU  - Alexandre Chuyen
AU  - Fabrice Daian
AU  - Andrea Pasini
AU  - Laurent Kodjabachian
PY  - 2026
DA  - 2026/10/09
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2021.100928
UR  - https://hal.science/hal-05790503v1
AB  - A live-imaging protocol to track cell movement in the Xenopus embryo Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded.
SN  - 2666-1667
ER  -