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Peer-reviewedCC BY 4.0STAR Protocols

Protocol for imaging and analyzing the synaptic coverage of astrocytes with super-resolution STED microscopy in mouse brain slices

  • Lucy Waldron1,2
  • Maïna Garnero1,3
  • David Mazaud1
  • Nathalie Rouach1
  1. 1Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology, Collège de France, CNRS, INSERM, PSL-NEURO, Université PSL, 75005 Paris, France
  2. 2Doctoral School SdV N°657, Université PSL, Paris, France
  3. 3Doctoral School N°158, Sorbonne Université, Paris, France
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Abstract

Super-resolution has allowed us to image areas previously inaccessible due to the limits of standard microscopy techniques. Here, we present a protocol to determine the astroglial coverage of synapses. We detail the steps to obtain and label mouse brain slices followed by imaging using 3D stimulated emission depletion (STED) microscopy and analysis using a custom-made Fiji plug-in, with imaging at a lateral resolution of 80 nm. This technique ascertains the interaction between astrocytes and the glial cell’s role in signaling and brain region function.

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Cite

BibTeXRIS
BibTeX
@article{waldron2026protocol,
  title = {{Protocol for imaging and analyzing the synaptic coverage of astrocytes with super-resolution STED microscopy in mouse brain slices}},
  author = {Lucy Waldron and Maïna Garnero and David Mazaud and Nathalie Rouach},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104906},
  url = {https://doi.org/10.1016/j.xpro.2026.104906},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Protocol for imaging and analyzing the synaptic coverage of astrocytes with super-resolution STED microscopy in mouse brain slices
AU  - Lucy Waldron
AU  - Maïna Garnero
AU  - David Mazaud
AU  - Nathalie Rouach
PY  - 2026
DA  - 2026/10/21
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104906
UR  - https://doi.org/10.1016/j.xpro.2026.104906
AB  - Super-resolution has allowed us to image areas previously inaccessible due to the limits of standard microscopy techniques. Here, we present a protocol to determine the astroglial coverage of synapses. We detail the steps to obtain and label mouse brain slices followed by imaging using 3D stimulated emission depletion (STED) microscopy and analysis using a custom-made Fiji plug-in, with imaging at a lateral resolution of 80 nm. This technique ascertains the interaction between astrocytes and the glial cell’s role in signaling and brain region function.
SN  - 2666-1667
ER  -