Anti-Hyperglycaemic, Hepatoprotective, and Nephroprotective Effects of Wrightia tinctoria Seeds and Enterococcus ratti in Alloxan-Induced Diabetic Mice
- 1State Key Laboratory of Vegetation Structure, Functions and Construction, and Yunnan Key Laboratory of Soil Erosion Prevention and Green Development, , Kunming 650500, China;
Abstract
Diabetes mellitus (DM), a chronic metabolic disease that leads to damage to multiple organ systems, needs to be investigated in natural ways. The present experiment was performed to determine the anti-hyperglycaemic, nephroprotective and hepatoprotective potential of the methanolic extract of Wrightia tinctoria (W. tinctoria) seeds, and Enterococcus ratti (E. ratti), in alloxan-induced hyperglycaemic mice (150 mg/kg body weight). W. tinctoria and E. ratti were screened out from different locally medicinally important plants and bacterial strains by different in vitro tests. W. tinctoria seeds showed α-amylase inhibition through the starch–iodine test (88.64% at 100 mg/mL), inhibition of glycosylation of haemoglobin (88.34% at 3.56 mg/mL), and an inhibitory effect on glucose uptake by yeast cells (91.62% at 500 µg/mL). Among various bacterial strains used for in vitro screening, E. ratti exhibited the most effective glucose adsorption capacity (26.2% at colony-forming unit (CFU) 1011). High-performance liquid chromatography (HPLC) and Fourier-transformed infrared (FT-IR) spectroscopy analyses provided preliminary information on the phytochemical composition of the extract. In vivo administration of W. tinctoria (200, 400 mg/kg body weight), E. ratti (60 µL at a CFU 1011) alone and their combination produced significant dose-dependent improvements in treatment groups, with the combination demonstrating enhanced efficacy compared to individual treatments. Future research should focus on detailed mechanisms underlying these protective effects using transcriptomic and proteomic approaches and developing standardized formulation strategies for translation into clinical applications.
Related
- Stress hyperglycaemic ratio is independently associated with intramyocardial haemorrhage in STEMI patients: a cardiac magnetic resonance study.
- Anti-diabetic and Antioxidant Activities of a Bornean Ethnomedicinal Plant: In vitro and In silico Investigations of Fibraurea tinctoria Root Fractions.
- Hepatoprotective Formula 919 ameliorates hepatic steatosis and metabolic disturbance in NAFLD with involvement of AMPK signaling
- Nephroprotective Effect of the Methanolic Extract of Flourensia cernua Against Renal Ischemia–Reperfusion Injury Associated with Modulation of Oxidative Stress in Wistar Rats
- Nephroprotective effects of GLP-1 receptor agonists in patients receiving lithium treatment: a multinational cohort study using the TriNetX network
BibTeX
@article{munawar2026antihyperglycaemic,
title = {{Anti-Hyperglycaemic, Hepatoprotective, and Nephroprotective Effects of Wrightia tinctoria Seeds and Enterococcus ratti in Alloxan-Induced Diabetic Mice}},
author = {Mohsin Munawar and Syed Turab Raza},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198792},
url = {https://europepmc.org/article/PMC/PMC13665475},
note = {CC BY},
}
RIS
TY - JOUR
TI - Anti-Hyperglycaemic, Hepatoprotective, and Nephroprotective Effects of Wrightia tinctoria Seeds and Enterococcus ratti in Alloxan-Induced Diabetic Mice
AU - Mohsin Munawar
AU - Syed Turab Raza
PY - 2026
DA - 2026/09/30
JO - International journal of molecular sciences
DO - 10.3390/ijms27198792
UR - https://europepmc.org/article/PMC/PMC13665475
AB - Diabetes mellitus (DM), a chronic metabolic disease that leads to damage to multiple organ systems, needs to be investigated in natural ways. The present experiment was performed to determine the anti-hyperglycaemic, nephroprotective and hepatoprotective potential of the methanolic extract of Wrightia tinctoria (W. tinctoria) seeds, and Enterococcus ratti (E. ratti), in alloxan-induced hyperglycaemic mice (150 mg/kg body weight). W. tinctoria and E. ratti were screened out from different locally medicinally important plants and bacterial strains by different in vitro tests. W. tinctoria seeds showed α-amylase inhibition through the starch–iodine test (88.64% at 100 mg/mL), inhibition of glycosylation of haemoglobin (88.34% at 3.56 mg/mL), and an inhibitory effect on glucose uptake by yeast cells (91.62% at 500 µg/mL). Among various bacterial strains used for in vitro screening, E. ratti exhibited the most effective glucose adsorption capacity (26.2% at colony-forming unit (CFU) 1011). High-performance liquid chromatography (HPLC) and Fourier-transformed infrared (FT-IR) spectroscopy analyses provided preliminary information on the phytochemical composition of the extract. In vivo administration of W. tinctoria (200, 400 mg/kg body weight), E. ratti (60 µL at a CFU 1011) alone and their combination produced significant dose-dependent improvements in treatment groups, with the combination demonstrating enhanced efficacy compared to individual treatments. Future research should focus on detailed mechanisms underlying these protective effects using transcriptomic and proteomic approaches and developing standardized formulation strategies for translation into clinical applications.
SN - 1422-0067
ER -