Anti-diabetic and Antioxidant Activities of a Bornean Ethnomedicinal Plant: In vitro and In silico Investigations of Fibraurea tinctoria Root Fractions.
- 1Department of Pharmacy, Faculty of Medicine, University of Islam Malang, Malang, Indonesia.
- 2Research Center for Genetic Engineering, Research Organization for Life Science and Environment, National Research and Innovation Agency (BRIN), KST Soekarno, Cibinong, Indonesia.
- 3Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makasar, Makasar, Indonesia.
Abstract
Fibraurea tinctoria, a native ethnomedicinal plant from Borneo, has been traditionally used to treat diabetes, with preclinical studies suggesting anti-hyperglycemic activity. However, the mechanisms underlying its hypoglycemic effects and active compounds remain unclear. This study aims to evaluate the anti-diabetic potential of F. tinctoria root extract through in vitro α-glucosidase inhibition and antioxidant assays, supported by in silico analysis. The roots were extracted using methanol, followed by fractionation with n-hexane (Fraction C6), ethyl acetate (Fraction EtOAc), n-butanol (Fraction BuOH) and aqueous solvent (Fraction H2O). The α-glucosidase inhibition assay was conducted using p-nitrophenyl-α-D-glucopyranoside (p-NPG), and antioxidant activity was measured using the DPPH method. Data were presented as mean ± SD, with IC50 values calculated by linear regression. Fraction EtOAc exhibited the strongest inhibitory activity against both α-glucosidase (IC50 = 147 ± 3.18 μg/mL) and free radicals (IC50 = 155 ± 2.30 μg/mL) compared with other fractions, though lower than reference standard acarbose and ascorbic acid. Phytochemical analysis using GC-MS identified bioactive compounds, further confirmed by molecular docking. 3-Isoxazolecarboperoxoic acid demonstrated optimal interactions with α-glucosidase (3TOP) and the aldose reductase (1IEI). These findings suggest that F. tinctoria could be a promising source for novel anti-diabetic and antioxidant agents.
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BibTeX
@article{purnomo2026antidiabetic,
title = {{Anti-diabetic and Antioxidant Activities of a Bornean Ethnomedicinal Plant: In vitro and In silico Investigations of Fibraurea tinctoria Root Fractions.}},
author = {Yudi Purnomo and Apon Zaenal Mustopa and Jendri Mamangkey and Herman Irawan},
journal = {Tropical life sciences research},
year = {2026},
month = oct,
doi = {10.21315/tlsr2026.37.3.10},
url = {https://europepmc.org/article/MED/42997720},
note = {CC BY},
}
RIS
TY - JOUR
TI - Anti-diabetic and Antioxidant Activities of a Bornean Ethnomedicinal Plant: In vitro and In silico Investigations of Fibraurea tinctoria Root Fractions.
AU - Yudi Purnomo
AU - Apon Zaenal Mustopa
AU - Jendri Mamangkey
AU - Herman Irawan
PY - 2026
DA - 2026/10/15
JO - Tropical life sciences research
DO - 10.21315/tlsr2026.37.3.10
UR - https://europepmc.org/article/MED/42997720
AB - Fibraurea tinctoria, a native ethnomedicinal plant from Borneo, has been traditionally used to treat diabetes, with preclinical studies suggesting anti-hyperglycemic activity. However, the mechanisms underlying its hypoglycemic effects and active compounds remain unclear. This study aims to evaluate the anti-diabetic potential of F. tinctoria root extract through in vitro α-glucosidase inhibition and antioxidant assays, supported by in silico analysis. The roots were extracted using methanol, followed by fractionation with n-hexane (Fraction C6), ethyl acetate (Fraction EtOAc), n-butanol (Fraction BuOH) and aqueous solvent (Fraction H2O). The α-glucosidase inhibition assay was conducted using p-nitrophenyl-α-D-glucopyranoside (p-NPG), and antioxidant activity was measured using the DPPH method. Data were presented as mean ± SD, with IC50 values calculated by linear regression. Fraction EtOAc exhibited the strongest inhibitory activity against both α-glucosidase (IC50 = 147 ± 3.18 μg/mL) and free radicals (IC50 = 155 ± 2.30 μg/mL) compared with other fractions, though lower than reference standard acarbose and ascorbic acid. Phytochemical analysis using GC-MS identified bioactive compounds, further confirmed by molecular docking. 3-Isoxazolecarboperoxoic acid demonstrated optimal interactions with α-glucosidase (3TOP) and the aldose reductase (1IEI). These findings suggest that F. tinctoria could be a promising source for novel anti-diabetic and antioxidant agents.
SN - 1985-3718
SN - 2180-4249
ER -