Comprehensive UPLC-QTOF-MS based metabolic profiling and insights into the bioactive potential of Vanda tessellata: a medicinal orchid
- 1Centre for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be University)
- 2Department of Biotechnology, Rama Devi Women’s University
Abstract
Vanda tessellata (Roxb.) Hook. Ex Don. is a medicinal epiphytic orchid traditionally used to treat inflammation, rheumatism, bronchitis, and other neurological disorders. The present study aimed to investigate the phytochemical composition and evaluate the antioxidant, antityrosinase, antibacterial, and antiproliferative activities of the methanolic extract of Vanda tessellata (VTE). Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) was employed to identify the phytoconstituents of VTE tentatively. The phenolic and flavonoid contents were assessed as gallic acid equivalents (GAE) and quercetin equivalents (QE), respectively. The evaluation of antioxidant activity determined using DPPH and ABTS radical scavenging assays. Antityrosinase activity was determined using the mushroom tyrosinase inhibition assay, while antibacterial activity was measured using both well diffusion and broth microdilution methods. Additionally, antiproliferative activity was investigated against Panc-1, HepG2, PC3, and HEK-293 cell lines using the MTT assay. The extract exhibited significant total phenolic and flavonoid content, measuring 95.72 µg GAE/mg of extract and 113.49 µg QE/mg of extract, respectively. Comprehensive UHPLC-QTOF-MS analysis tentatively identified 33 metabolites, predominantly flavonoids, phenolic acids, carboxylic acids, terpenoids, and alkaloids. VTE showed significant antioxidant activity, with IC₅₀ values of 59.38 ± 0.27 µg/mL and 27.63 ± 0.14 µg/mL in DPPH and ABTS assays, respectively. The extract also exhibited moderate tyrosinase inhibitory activity (IC₅₀ = 235.36 µg/mL), indicating its potential relevance to regulating melanin biosynthesis. Antibacterial evaluation revealed moderate activity against Bacillus cereus and Enterococcus faecalis, with MIC values of 62.5 µg/mL. Furthermore, VTE demonstrated selective antiproliferative activity against Panc-1, HepG2, and PC3 cancer cell lines with IC₅₀ values of 31.16, 54.09, and 62.24 µg/mL, respectively, while exhibiting substantially lower cytotoxicity toward normal HEK-293 cells (IC₅₀ = 221.47 µg/mL). Overall, the findings provide scientific evidence supporting the pharmacological potential of V. tessellata and highlight its promise as a natural source of antioxidant, tyrosinase inhibitory, anticancer, and antibacterial agents.
Related
- The Bioactive Potential of Algae and Their Health-Promoting Properties in the Context of the Impact on Human Health and Potential Applications in Food Technology: A Comprehensive Review
- Ethnomedicinal Uses, Phytochemistry and Bioactivities of Halophytes and Salt-Tolerant Plants with Potential Against Metabolic Syndrome: A Comprehensive Review
- Comparative profiling of bioactive compounds and antioxidant capacities of Brazilian semiarid Spondias species
- Persimmon By-Products: Bioactive Phytochemicals, Sustainable Valorization and Potential Food and Health Applications
- Potential role of resveratrol in abdominal cocoon syndrome: targeting inflammation, oxidative stress, and fibrosis via food-derived bioactive compounds resveratrol for treatment of abdominal cocoon syndrome
BibTeX
@article{padhee2026comprehensive,
title = {{Comprehensive UPLC-QTOF-MS based metabolic profiling and insights into the bioactive potential of Vanda tessellata: a medicinal orchid}},
author = {Sucheesmita Padhee and Rout George Kerry and Debajani Mohanty and Prabhat Kumar Das and Ambika Sahoo and Sudipta Jena and Sujata Mohanty and Laxmikanta Acharya and Pratap Chandra Panda and Asit Ray and Sanghamitra Nayak},
journal = {Scientific Reports},
year = {2026},
month = jul,
doi = {10.1038/s41598-026-63072-3},
url = {https://doi.org/10.1038/s41598-026-63072-3},
note = {CC BY},
}
RIS
TY - JOUR
TI - Comprehensive UPLC-QTOF-MS based metabolic profiling and insights into the bioactive potential of Vanda tessellata: a medicinal orchid
AU - Sucheesmita Padhee
AU - Rout George Kerry
AU - Debajani Mohanty
AU - Prabhat Kumar Das
AU - Ambika Sahoo
AU - Sudipta Jena
AU - Sujata Mohanty
AU - Laxmikanta Acharya
AU - Pratap Chandra Panda
AU - Asit Ray
AU - Sanghamitra Nayak
PY - 2026
DA - 2026/07/01
JO - Scientific Reports
DO - 10.1038/s41598-026-63072-3
UR - https://doi.org/10.1038/s41598-026-63072-3
AB - Vanda tessellata (Roxb.) Hook. Ex Don. is a medicinal epiphytic orchid traditionally used to treat inflammation, rheumatism, bronchitis, and other neurological disorders. The present study aimed to investigate the phytochemical composition and evaluate the antioxidant, antityrosinase, antibacterial, and antiproliferative activities of the methanolic extract of Vanda tessellata (VTE). Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) was employed to identify the phytoconstituents of VTE tentatively. The phenolic and flavonoid contents were assessed as gallic acid equivalents (GAE) and quercetin equivalents (QE), respectively. The evaluation of antioxidant activity determined using DPPH and ABTS radical scavenging assays. Antityrosinase activity was determined using the mushroom tyrosinase inhibition assay, while antibacterial activity was measured using both well diffusion and broth microdilution methods. Additionally, antiproliferative activity was investigated against Panc-1, HepG2, PC3, and HEK-293 cell lines using the MTT assay. The extract exhibited significant total phenolic and flavonoid content, measuring 95.72 µg GAE/mg of extract and 113.49 µg QE/mg of extract, respectively. Comprehensive UHPLC-QTOF-MS analysis tentatively identified 33 metabolites, predominantly flavonoids, phenolic acids, carboxylic acids, terpenoids, and alkaloids. VTE showed significant antioxidant activity, with IC₅₀ values of 59.38 ± 0.27 µg/mL and 27.63 ± 0.14 µg/mL in DPPH and ABTS assays, respectively. The extract also exhibited moderate tyrosinase inhibitory activity (IC₅₀ = 235.36 µg/mL), indicating its potential relevance to regulating melanin biosynthesis. Antibacterial evaluation revealed moderate activity against Bacillus cereus and Enterococcus faecalis, with MIC values of 62.5 µg/mL. Furthermore, VTE demonstrated selective antiproliferative activity against Panc-1, HepG2, and PC3 cancer cell lines with IC₅₀ values of 31.16, 54.09, and 62.24 µg/mL, respectively, while exhibiting substantially lower cytotoxicity toward normal HEK-293 cells (IC₅₀ = 221.47 µg/mL). Overall, the findings provide scientific evidence supporting the pharmacological potential of V. tessellata and highlight its promise as a natural source of antioxidant, tyrosinase inhibitory, anticancer, and antibacterial agents.
SN - 2045-2322
ER -