Micropropagation of Lisianthus (Eustoma grandiflorum (Raf.) Shinn.) Using Meta-Topolin.
4 affiliations
- 1Centro Nacional de Recursos Genéticos, INIFAP, Boulevard de la Biodiversidad No. 400, Rancho las Cruces, 2498, Tepatitlán de Morelos 47600, Jalisco, Mexico.
- 2Colegio de Postgraduados Campus Córdoba, Km. 348, Carretera Federal Córdoba-Veracruz, Manuel León, Amatlán de los Reyes 94953, Veracruz, Mexico.
- 3Facultad de Ciencias Agrícolas, Universidad Veracruzana, Circuito Gonzalo Aguirre Beltrán, Zona Universitaria, Xalapa 91000, Veracruz, Mexico.
- 4Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Peñuela, Amatlán de los Reyes 94945, Veracruz, Mexico.
Abstract
Lisianthus (Eustoma grandiflorum) is an ornamental species of high commercial value due to its diverse flower colors and prolonged vase life. However, lisianthus propagation by seed is limited by its low germination rates and high genetic variability, compromising plant uniformity and production timelines. This study evaluated the effect of meta-Topolin (mT) and of 6-benzylaminopurine (BAP), aromatic cytokinins, on the in vitro micropropagation of lisianthus, aiming to develop an efficient protocol for mass propagation. In vitro established shoots were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of mT and BAP (0, 0.5, 1.0, or 1.5 mg L⁻¹). The parameters assessed were shoot proliferation, shoot length, leaf number, root formation, and root length. Significant differences were observed among treatments. The highest proliferation rate (59.20 shoots per explant) was achieved with 0.5 mg L⁻¹ mT, representing a 37-fold increase over the control. With the addition of BAP to the culture medium, the results were inferior. Higher mT concentrations promoted rooting and improved acclimatization success, with the highest survival rate (95%) recorded for plants from the 1.5 mg L⁻¹ mT treatment. Low mT concentrations enhanced shoot multiplication but limited root formation, affecting nursery survival. Results demonstrated that meta-Topolin induces a higher shoot number during lisianthus micropropagation, and at higher concentrations, it enables in vitro rooting of the shoots generated during the proliferation stage. This provides a practical alternative for large-scale commercial propagation of this ornamental species.
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BibTeX
@article{ramirezmosqueda2026micropropagation,
title = {{Micropropagation of Lisianthus (Eustoma grandiflorum (Raf.) Shinn.) Using Meta-Topolin.}},
author = {Marco A Ramírez-Mosqueda and Alma Armenta-Medina and Obdulia Baltazar-Bernal and José Roberto Bautista-Aguilar and Marco Vinicio Rodríguez-Deméneghi},
journal = {Biotech (Basel (Switzerland))},
year = {2026},
month = oct,
doi = {10.3390/biotech15040093},
url = {https://europepmc.org/article/MED/42930070},
note = {CC BY},
}
RIS
TY - JOUR
TI - Micropropagation of Lisianthus (Eustoma grandiflorum (Raf.) Shinn.) Using Meta-Topolin.
AU - Marco A Ramírez-Mosqueda
AU - Alma Armenta-Medina
AU - Obdulia Baltazar-Bernal
AU - José Roberto Bautista-Aguilar
AU - Marco Vinicio Rodríguez-Deméneghi
PY - 2026
DA - 2026/10/13
JO - Biotech (Basel (Switzerland))
DO - 10.3390/biotech15040093
UR - https://europepmc.org/article/MED/42930070
AB - Lisianthus (Eustoma grandiflorum) is an ornamental species of high commercial value due to its diverse flower colors and prolonged vase life. However, lisianthus propagation by seed is limited by its low germination rates and high genetic variability, compromising plant uniformity and production timelines. This study evaluated the effect of meta-Topolin (mT) and of 6-benzylaminopurine (BAP), aromatic cytokinins, on the in vitro micropropagation of lisianthus, aiming to develop an efficient protocol for mass propagation. In vitro established shoots were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of mT and BAP (0, 0.5, 1.0, or 1.5 mg L⁻¹). The parameters assessed were shoot proliferation, shoot length, leaf number, root formation, and root length. Significant differences were observed among treatments. The highest proliferation rate (59.20 shoots per explant) was achieved with 0.5 mg L⁻¹ mT, representing a 37-fold increase over the control. With the addition of BAP to the culture medium, the results were inferior. Higher mT concentrations promoted rooting and improved acclimatization success, with the highest survival rate (95%) recorded for plants from the 1.5 mg L⁻¹ mT treatment. Low mT concentrations enhanced shoot multiplication but limited root formation, affecting nursery survival. Results demonstrated that meta-Topolin induces a higher shoot number during lisianthus micropropagation, and at higher concentrations, it enables in vitro rooting of the shoots generated during the proliferation stage. This provides a practical alternative for large-scale commercial propagation of this ornamental species.
SN - 2673-6284
ER -