An In Vivo Study on Wound Healing Activity of Prangos Species and Phytochemical Investigations
6 affiliations
- 1Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, Ankara 06560, Türkiye
- 2Graduate School of Health Sciences, Ankara University, Dışkapı, Ankara 06110, Türkiye
- 3Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, Ankara 06330, Türkiye
- 4Department of Pharmaceutical Basic Sciences, Faculty of Pharmacy, Gazi University, Ankara 06630, Türkiye
- 5Department of Pathology, Faculty of Veterinary Medicine, Fırat University, Elazığ 23119, Türkiye
- 6Department of Pharmacognosy, Faculty of Pharmacy, Adıyaman University, Adıyaman 02040, Türkiye
Abstract
Wound healing is a dynamic and highly coordinated biological process involving four overlapping phases: hemostasis, inflammation, proliferation, and tissue remodeling. The genus Prangos is known as “Çakşır” in Turkish and is an ethnomedicinal plant traditionally used in Anatolia for many purposes, including wound healing. The plant species is rich in furanocoumarins and their derivatives. This study aimed to investigate the wound-healing activity of selected endemic Prangos species, which are frequently mentioned in traditional medicine. For this purpose, several endemic species, such as Prangos abieticola Aytaç & H. Duman, Prangos denticulata Fisch. & C.A. Mey., Prangos heyniae H. Duman & M.F. Watson, and Prangos uechtritzii Boiss. & Hausskn. were collected and extracted for comparison of in vivo wound-healing activities. We evaluated the wound-healing activity of methanol extracts of the aerial parts of these species using a circular excision wound model. Tissue levels of TGF-β, hydroxyproline, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were measured. The methanol extract of P. denticulata exhibited notable wound-healing activity. Therefore, in this study, P. denticulata was selected as a candidate for phytochemical investigations, and the methanol extract of the species was subjected to a successive chromatographic process. Phytochemical studies yielded seven known furanocoumarin-type compounds (1–9): isoimperatorin (1), imperatorin (2), oxypeucedanin methanolate (3), xanthotoxol (4), isogospherol (5), saxalin (6), and isosaxalin (7), along with a simple coumarin, ulopterol (8), and a linear-type dihydropyranocoumarin called decursinol angelate/agasyllin (9). The structures were elucidated using 1H, 13C, and 2D NMR spectroscopy, as well as MS fragmentation. This study reports the wound-healing activities of endemic species growing in Anatolian flora, along with the phytochemical content of the active species, P. denticulata. Since coumarin-type compounds are specific and dominant in Prangos species, it is considered that the wound-healing activity could be attributed to these compounds.
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BibTeX
@article{gulmez2026vivo,
title = {{An In Vivo Study on Wound Healing Activity of Prangos Species and Phytochemical Investigations}},
author = {Nazif Gülmez and Alev Onder and İpek Süntar and Bayram Kagan Akay and Mürşide Ayşe Demirel and Songül Çeribaşı and Erkan Yilmaz},
journal = {Molecules},
year = {2026},
month = nov,
doi = {10.3390/molecules31213974},
url = {https://www.mdpi.com/1420-3049/31/21/3974},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - An In Vivo Study on Wound Healing Activity of Prangos Species and Phytochemical Investigations
AU - Nazif Gülmez
AU - Alev Onder
AU - İpek Süntar
AU - Bayram Kagan Akay
AU - Mürşide Ayşe Demirel
AU - Songül Çeribaşı
AU - Erkan Yilmaz
PY - 2026
DA - 2026/11/04
JO - Molecules
DO - 10.3390/molecules31213974
UR - https://www.mdpi.com/1420-3049/31/21/3974
AB - Wound healing is a dynamic and highly coordinated biological process involving four overlapping phases: hemostasis, inflammation, proliferation, and tissue remodeling. The genus Prangos is known as “Çakşır” in Turkish and is an ethnomedicinal plant traditionally used in Anatolia for many purposes, including wound healing. The plant species is rich in furanocoumarins and their derivatives. This study aimed to investigate the wound-healing activity of selected endemic Prangos species, which are frequently mentioned in traditional medicine. For this purpose, several endemic species, such as Prangos abieticola Aytaç & H. Duman, Prangos denticulata Fisch. & C.A. Mey., Prangos heyniae H. Duman & M.F. Watson, and Prangos uechtritzii Boiss. & Hausskn. were collected and extracted for comparison of in vivo wound-healing activities. We evaluated the wound-healing activity of methanol extracts of the aerial parts of these species using a circular excision wound model. Tissue levels of TGF-β, hydroxyproline, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were measured. The methanol extract of P. denticulata exhibited notable wound-healing activity. Therefore, in this study, P. denticulata was selected as a candidate for phytochemical investigations, and the methanol extract of the species was subjected to a successive chromatographic process. Phytochemical studies yielded seven known furanocoumarin-type compounds (1–9): isoimperatorin (1), imperatorin (2), oxypeucedanin methanolate (3), xanthotoxol (4), isogospherol (5), saxalin (6), and isosaxalin (7), along with a simple coumarin, ulopterol (8), and a linear-type dihydropyranocoumarin called decursinol angelate/agasyllin (9). The structures were elucidated using 1H, 13C, and 2D NMR spectroscopy, as well as MS fragmentation. This study reports the wound-healing activities of endemic species growing in Anatolian flora, along with the phytochemical content of the active species, P. denticulata. Since coumarin-type compounds are specific and dominant in Prangos species, it is considered that the wound-healing activity could be attributed to these compounds.
SN - 1420-3049
ER -