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Peer-reviewedCC BYData in Brief

Dataset of full-length transcriptome assembly and annotation of a two-year-old

  • Yanxia Gao1
  • Binbin Wang1
  • Jing Wang1
  • Yan Xie1
  • Jiahui Wang2
  • Lan Yang3
  • Gaixia Zhang4
  • Hui Wang5
5 affiliations
  1. 1Sericulture Research Institute, Chengde Medical University, Chengde, 067000, China
  2. 2School of Basic Medical Sciences, Chengde Medical University, Chengde, 067000, China
  3. 3Department of Traditional Chinense Medicine, Chengde Medical University, Chengde, 067000, China
  4. 4College of Pharmacy, Shandong University of Traditional Chinense Medicine, Jinan, 250355, China
  5. 5Sericulture Research Institute, Chengde Medical University, Chengde, 067000, China; Corresponding author.
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Abstract

Transcriptome sequencing has become an important tool for gene discovery in medicinal plants. Compared with next-generation sequencing (NGS), third-generation sequencing, also known as single-molecule real-time (SMRT) sequencing, provides longer reads and avoids PCR amplification, making it particularly suitable for full-length transcriptome analysis. Bupleurum chinense DC. is an important medicinal herb in China. However, genome information for B. chinense remains limited. In this study, total RNA was separately extracted from the root, stem, and leaf of a two-year-old B. chinense plant, and equal amounts of RNA from the three tissues were pooled for PacBio SMRT full-length transcriptome sequencing. This study generated the first full-length transcriptome dataset for B. chinense . The dataset includes 11,448 annotated unigenes, 690 transcription factors, and 6178 SSR markers, with many unigenes associated with terpenoid biosynthesis and other secondary metabolism pathways. The raw data were deposited in the SRA database under BioProject accession number PRJNA1494021. This dataset will facilitate gene discovery, functional characterization, saikosaponin biosynthesis research, marker development, and molecular breeding in B. chinense and related species.

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Cite

BibTeXRIS
BibTeX
@article{gao2026dataset,
  title = {{Dataset of full-length transcriptome assembly and annotation of a two-year-old}},
  author = {Yanxia Gao and Binbin Wang and Jing Wang and Yan Xie and Jiahui Wang and Lan Yang and Gaixia Zhang and Hui Wang},
  journal = {Data in Brief},
  year = {2026},
  month = aug,
  doi = {10.1016/j.dib.2026.113347},
  url = {https://doi.org/10.1016/j.dib.2026.113347},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Dataset of full-length transcriptome assembly and annotation of a two-year-old
AU  - Yanxia Gao
AU  - Binbin Wang
AU  - Jing Wang
AU  - Yan Xie
AU  - Jiahui Wang
AU  - Lan Yang
AU  - Gaixia Zhang
AU  - Hui Wang
PY  - 2026
DA  - 2026/08/01
JO  - Data in Brief
DO  - 10.1016/j.dib.2026.113347
UR  - https://doi.org/10.1016/j.dib.2026.113347
AB  - Transcriptome sequencing has become an important tool for gene discovery in medicinal plants. Compared with next-generation sequencing (NGS), third-generation sequencing, also known as single-molecule real-time (SMRT) sequencing, provides longer reads and avoids PCR amplification, making it particularly suitable for full-length transcriptome analysis. Bupleurum chinense DC. is an important medicinal herb in China. However, genome information for B. chinense remains limited. In this study, total RNA was separately extracted from the root, stem, and leaf of a two-year-old B. chinense plant, and equal amounts of RNA from the three tissues were pooled for PacBio SMRT full-length transcriptome sequencing. This study generated the first full-length transcriptome dataset for B. chinense . The dataset includes 11,448 annotated unigenes, 690 transcription factors, and 6178 SSR markers, with many unigenes associated with terpenoid biosynthesis and other secondary metabolism pathways. The raw data were deposited in the SRA database under BioProject accession number PRJNA1494021. This dataset will facilitate gene discovery, functional characterization, saikosaponin biosynthesis research, marker development, and molecular breeding in B. chinense and related species.
SN  - 2352-3409
ER  -