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Peer-reviewedCC BY 4.0Journal of Microscopy

Single‐molecule force spectroscopy of biomolecules using AFM. A multigenerational perspective

  • Ismahene Mesbah
  • Claire Valotteau
  • Felix Rico
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Abstract

Single‐molecule force spectroscopy (SMFS) using atomic force microscopy (AFM) allows the direct application and measurement of forces on individual molecular systems; revolutionizing our understanding of biomolecular mechanics and receptor‐ligand interactions and opening the field of single‐molecule mechanobiology. This review traces the evolution of the field, from foundational landmark experiments to recent developments that allowed revisiting those seminal works. We discuss the critical technological advancements, functionalisation strategies and theoretical developments that have shaped the discipline. Finally, we suggest future directions to further elucidate the intricate relationship between structure and mechanical properties of single biomolecules.

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Cite

BibTeXRIS
BibTeX
@article{mesbah2026singlemolecule,
  title = {{Single‐molecule force spectroscopy of biomolecules using AFM. A multigenerational perspective}},
  author = {Ismahene Mesbah and Claire Valotteau and Felix Rico},
  journal = {Journal of Microscopy},
  year = {2026},
  month = nov,
  doi = {10.1111/jmi.70196},
  url = {https://hal.science/hal-05852603v1},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Single‐molecule force spectroscopy of biomolecules using AFM. A multigenerational perspective
AU  - Ismahene Mesbah
AU  - Claire Valotteau
AU  - Felix Rico
PY  - 2026
DA  - 2026/11/01
JO  - Journal of Microscopy
DO  - 10.1111/jmi.70196
UR  - https://hal.science/hal-05852603v1
AB  - Single‐molecule force spectroscopy (SMFS) using atomic force microscopy (AFM) allows the direct application and measurement of forces on individual molecular systems; revolutionizing our understanding of biomolecular mechanics and receptor‐ligand interactions and opening the field of single‐molecule mechanobiology. This review traces the evolution of the field, from foundational landmark experiments to recent developments that allowed revisiting those seminal works. We discuss the critical technological advancements, functionalisation strategies and theoretical developments that have shaped the discipline. Finally, we suggest future directions to further elucidate the intricate relationship between structure and mechanical properties of single biomolecules.
SN  - 0022-2720
SN  - 1365-2818
ER  -