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Peer-reviewedCC BY 4.0Frontiers in Microbiology

Antibacterial efficacy and mechanism of action of VTC3-knockout Saccharomyces cerevisiae against multidrug- resistant pathogenic bacteria

  • Weibao Yang1,2
  • Mengkun Li1
  • Yu Zhang1
  • Kan Hu1
  • Bingjie Lu1
  • Ailin Yuan1
  • Zuobin Zhu3
  • Ying Li1,4
4 affiliations
  1. 1School of Medical Technology, Xuzhou Medical University, Xuzhou
  2. 2Suqian Zhongwu Hospital, Suqian
  3. 3Department of Genetics, Xuzhou Medical University, Xuzhou
  4. 4Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Department of Microbial and Biochemical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou
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Abstract

Introduction: Multidrug-resistant (MDR) bacterial infections are a global public health crisis, and traditional antibiotic therapies are becoming increasingly ineffective. Probiotics represent a promising strategy in this context.

Methods: In this study, we examined the antibacterial activity and mechanism of action of Saccharomyces cerevisiae with VTC3 gene knockout ( VTC3- KO).

Results: Growth curves, biofilm formation, antibacterial spectrum, and coculture experiments demonstrated that VTC3- KO S. cerevisiae effectively inhibited the growth of pathogens and significantly suppressed the biofilm formation of Escherichia coli , Staphylococcus aureus , and Klebsiella pneumoniae . Moreover, VTC3- KO S. cerevisiae exhibited tolerance to the gastrointestinal environment, with enhanced auto and coaggregation with pathogens. In in vivo culture of Galleria mellonella and in a mouse infection model, VTC3- KO S. cerevisiae significantly improved host survival, alleviated intestinal tissue damage, and reduced inflammatory cell infiltration.

Discussion: Mechanistic studies suggest that the observed antimicrobial effect may be attributable to the production of acidic substances. These findings demonstrate that VTC3- KO S. cerevisiae possesses dual antibacterial and probiotic functions, providing a sustainable alternative to traditional antibiotics for treating MDR infections.

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Cite

BibTeXRIS
BibTeX
@article{yang2026antibacterial,
  title = {{Antibacterial efficacy and mechanism of action of VTC3-knockout Saccharomyces cerevisiae against multidrug- resistant pathogenic bacteria}},
  author = {Weibao Yang and Mengkun Li and Yu Zhang and Kan Hu and Bingjie Lu and Ailin Yuan and Zuobin Zhu and Ying Li},
  journal = {Frontiers in Microbiology},
  year = {2026},
  month = oct,
  doi = {10.3389/fmicb.2026.1937861},
  url = {https://www.frontiersin.org/articles/10.3389/fmicb.2026.1937861/full},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Antibacterial efficacy and mechanism of action of VTC3-knockout Saccharomyces cerevisiae against multidrug- resistant pathogenic bacteria
AU  - Weibao Yang
AU  - Mengkun Li
AU  - Yu Zhang
AU  - Kan Hu
AU  - Bingjie Lu
AU  - Ailin Yuan
AU  - Zuobin Zhu
AU  - Ying Li
PY  - 2026
DA  - 2026/10/21
JO  - Frontiers in Microbiology
DO  - 10.3389/fmicb.2026.1937861
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2026.1937861/full
AB  - Introduction: Multidrug-resistant (MDR) bacterial infections are a global public health crisis, and traditional antibiotic therapies are becoming increasingly ineffective. Probiotics represent a promising strategy in this context.  Methods: In this study, we examined the antibacterial activity and mechanism of action of Saccharomyces cerevisiae with VTC3 gene knockout ( VTC3- KO).  Results: Growth curves, biofilm formation, antibacterial spectrum, and coculture experiments demonstrated that VTC3- KO S. cerevisiae effectively inhibited the growth of pathogens and significantly suppressed the biofilm formation of Escherichia coli , Staphylococcus aureus , and Klebsiella pneumoniae . Moreover, VTC3- KO S. cerevisiae exhibited tolerance to the gastrointestinal environment, with enhanced auto and coaggregation with pathogens. In in vivo culture of Galleria mellonella and in a mouse infection model, VTC3- KO S. cerevisiae significantly improved host survival, alleviated intestinal tissue damage, and reduced inflammatory cell infiltration.  Discussion: Mechanistic studies suggest that the observed antimicrobial effect may be attributable to the production of acidic substances. These findings demonstrate that VTC3- KO S. cerevisiae possesses dual antibacterial and probiotic functions, providing a sustainable alternative to traditional antibiotics for treating MDR infections.
SN  - 1664-302X
ER  -