GC-MS identification of a lacthydrazide-class siderophore produced by Streptomyces sp. VITGV100: its antimicrobial activity and molecular docking
- 1School of BioSciences and Technology, Vellore Institute of Technology, VIT University, Vellore, Tamil Nadu, India
Abstract
Background: The bacterial genus Streptomyces comprises well-known producers of bioactive compounds, mainly antibiotics. Specifically, the strain Streptomyces sp. VITGV100 shows promise as a potent antimicrobial source. This study investigates the activation of cryptic biosynthetic genes within this strain through chemical elicitation to amplify the production of secondary metabolites.
Materials and methods: To achieve this, Streptomyces sp. VITGV100 was cultured in nutrient broth supplemented with 0.5% dimethyl sulfoxide (DMSO) to elicit metabolic changes over 7, 14, and 21 days. The resulting crude extracts were screened for antimicrobial activity against four human pathogens: Escherichia coli (Microbial Type Culture Collection and Gene Bank [MTCC] 1687), Pseudomonas aeruginosa (MTCC 3541), Bacillus subtilis (MTCC 2756), and Staphylococcus aureus (MTCC 737). Bioactive fractions were characterized by gas chromatography-mass spectrometry (GC-MS). Finally, identified compounds, namely lacthydrazide, were docked against target proteins: E. coli Z-ring-associated protein (5IMJ), P. aeruginosa OXA10 (4WZ5), S. aureus TyrRS (1JIJ), and B. subtilis DLTA (3E7X).
Results: The 7-day crude extract exhibited the strongest antimicrobial activity, yielding a maximum inhibition zone of 31 mm against E. coli at 100 μg/ml and a minimum zone of 9 mm against B. subtilis at 25 μg/ml. GC-MS analysis revealed 45 distinct peaks in the included extracts versus 30 in controls, identifying lacthydrazide as a primary compound. Molecular docking confirmed the strong binding affinity of lacthydrazide to the target proteins, supporting its antimicrobial potential.
Conclusions: Streptomyces sp. VITGV100, elicited with 0.5% DMSO, effectively activated cryptic genes to synthesize potent antibacterial compounds. These findings position this strain as a valuable resource for discovering novel antimicrobials.
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BibTeX
@article{moon2026gcms,
title = {{GC-MS identification of a lacthydrazide-class siderophore produced by Streptomyces sp. VITGV100: its antimicrobial activity and molecular docking}},
author = {Madhuri Mukindrao Moon and John Godwin Christopher},
journal = {BioTechnologia},
year = {2026},
month = sep,
doi = {10.5114/bta/224355},
url = {https://doi.org/10.5114/bta/224355},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - GC-MS identification of a lacthydrazide-class siderophore produced by Streptomyces sp. VITGV100: its antimicrobial activity and molecular docking
AU - Madhuri Mukindrao Moon
AU - John Godwin Christopher
PY - 2026
DA - 2026/09/21
JO - BioTechnologia
DO - 10.5114/bta/224355
UR - https://doi.org/10.5114/bta/224355
AB - Background: The bacterial genus Streptomyces comprises well-known producers of bioactive compounds, mainly antibiotics. Specifically, the strain Streptomyces sp. VITGV100 shows promise as a potent antimicrobial source. This study investigates the activation of cryptic biosynthetic genes within this strain through chemical elicitation to amplify the production of secondary metabolites. Materials and methods: To achieve this, Streptomyces sp. VITGV100 was cultured in nutrient broth supplemented with 0.5% dimethyl sulfoxide (DMSO) to elicit metabolic changes over 7, 14, and 21 days. The resulting crude extracts were screened for antimicrobial activity against four human pathogens: Escherichia coli (Microbial Type Culture Collection and Gene Bank [MTCC] 1687), Pseudomonas aeruginosa (MTCC 3541), Bacillus subtilis (MTCC 2756), and Staphylococcus aureus (MTCC 737). Bioactive fractions were characterized by gas chromatography-mass spectrometry (GC-MS). Finally, identified compounds, namely lacthydrazide, were docked against target proteins: E. coli Z-ring-associated protein (5IMJ), P. aeruginosa OXA10 (4WZ5), S. aureus TyrRS (1JIJ), and B. subtilis DLTA (3E7X). Results: The 7-day crude extract exhibited the strongest antimicrobial activity, yielding a maximum inhibition zone of 31 mm against E. coli at 100 μg/ml and a minimum zone of 9 mm against B. subtilis at 25 μg/ml. GC-MS analysis revealed 45 distinct peaks in the included extracts versus 30 in controls, identifying lacthydrazide as a primary compound. Molecular docking confirmed the strong binding affinity of lacthydrazide to the target proteins, supporting its antimicrobial potential. Conclusions: Streptomyces sp. VITGV100, elicited with 0.5% DMSO, effectively activated cryptic genes to synthesize potent antibacterial compounds. These findings position this strain as a valuable resource for discovering novel antimicrobials.
SN - 0860-7796
SN - 2353-9461
ER -