Effects of 850-nm light-emitting diode photobiomodulation on pain intensity and dentin sialoprotein levels during canine distalization: A randomized controlled trial.
- 1Faculty of Dentistry, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
- 2Faculty of Dentistry, Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam.
- 3Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
Abstract
Background/purpose
Light-emitting diode-based photobiomodulation (LED PBM) potentially reduces pain and root resorption risk in orthodontics. This study evaluated 850-nm LED PBM effects on pain and dentin sialoprotein (DSP) levels during canine distalization.Materials and methods
This single-blind, parallel-group randomized controlled trial included 16 orthodontic patients (8 per group). Participants were randomly allocated in a 1:1 ratio to either an active LED group or a sham control group. Pain intensity was assessed using a visual analog scale (VAS) at 0, 24, and 48 h following canine distalization at four consecutive monthly visits (T₁-T₄) and expressed as the area under the curve (AUC0-48h). DSP concentrations in gingival crevicular fluid were measured at pretreatment (T₀), initiation of distalization (T₁), and follow-up visits (T₂, T₃) using sandwich ELISA. Longitudinal changes were analyzed using linear mixed-effects models.Results
The LED group demonstrated a significantly lower pain intensity compared with the control group, with a significant group-by-time interaction (χ²=9.46, P=0.024). Adjusted mean values of AUC0-48h were significantly reduced in the LED group at T₃ and T₄, with the greatest difference observed at T₃ (P < 0.001). A significant group-by-time interaction was observed for DSP (χ² = 9.75, P = 0.021), with lower DSP levels in the LED group being reported at T₁, T₂, and T₃ compared with the control group (P < 0.05).Conclusion
Repeated 850-nm LED PBM was associated with reduced cumulative pain and attenuated DSP increases during canine distalization, suggesting potential benefits for patient comfort and root resorption-related responses.
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BibTeX
@article{cao2026effects,
title = {{Effects of 850-nm light-emitting diode photobiomodulation on pain intensity and dentin sialoprotein levels during canine distalization: A randomized controlled trial.}},
author = {Thanh Thuy-Nhat Cao and Anh Ho-Quynh Nguyen and Hung Trong Hoang and Tham Dong Khac and Phuc Hoai Le and H K Tu Trinh and Kieu-Minh Le and Diem Truong},
journal = {Journal of dental sciences},
year = {2026},
month = oct,
doi = {10.1016/j.jds.2025.07.3502},
url = {https://europepmc.org/article/MED/42923675},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Effects of 850-nm light-emitting diode photobiomodulation on pain intensity and dentin sialoprotein levels during canine distalization: A randomized controlled trial.
AU - Thanh Thuy-Nhat Cao
AU - Anh Ho-Quynh Nguyen
AU - Hung Trong Hoang
AU - Tham Dong Khac
AU - Phuc Hoai Le
AU - H K Tu Trinh
AU - Kieu-Minh Le
AU - Diem Truong
PY - 2026
DA - 2026/10/01
JO - Journal of dental sciences
DO - 10.1016/j.jds.2025.07.3502
UR - https://europepmc.org/article/MED/42923675
AB - Background/purpose Light-emitting diode-based photobiomodulation (LED PBM) potentially reduces pain and root resorption risk in orthodontics. This study evaluated 850-nm LED PBM effects on pain and dentin sialoprotein (DSP) levels during canine distalization.Materials and methods This single-blind, parallel-group randomized controlled trial included 16 orthodontic patients (8 per group). Participants were randomly allocated in a 1:1 ratio to either an active LED group or a sham control group. Pain intensity was assessed using a visual analog scale (VAS) at 0, 24, and 48 h following canine distalization at four consecutive monthly visits (T₁-T₄) and expressed as the area under the curve (AUC0-48h). DSP concentrations in gingival crevicular fluid were measured at pretreatment (T₀), initiation of distalization (T₁), and follow-up visits (T₂, T₃) using sandwich ELISA. Longitudinal changes were analyzed using linear mixed-effects models.Results The LED group demonstrated a significantly lower pain intensity compared with the control group, with a significant group-by-time interaction (χ²=9.46, P=0.024). Adjusted mean values of AUC0-48h were significantly reduced in the LED group at T₃ and T₄, with the greatest difference observed at T₃ (P < 0.001). A significant group-by-time interaction was observed for DSP (χ² = 9.75, P = 0.021), with lower DSP levels in the LED group being reported at T₁, T₂, and T₃ compared with the control group (P < 0.05).Conclusion Repeated 850-nm LED PBM was associated with reduced cumulative pain and attenuated DSP increases during canine distalization, suggesting potential benefits for patient comfort and root resorption-related responses.
SN - 1991-7902
SN - 2213-8862
ER -