Dendritic cells and vascular inflammation in patients with coronary artery disease with and without chronic kidney disease
- 1Universitatsklinikum Jena
Abstract
ObjectiveDendritic cells (DCs) are priming local immune responses, and their tissue-associated activity increases in inflammatory conditions. In patients with coronary artery disease (CAD), circulating dendritic cell precursors (DCPs) are reduced. This study investigates circulating DCPs and tissue densities of migrated DCs in CAD patients undergoing bypass surgery, focussing on patients with chronic kidney disease (CKD) as a risk factor for CAD and a condition of chronic vascular inflammation.
Approach and ResultsCirculating DCPs were measured in the peripheral blood (before surgery) using fluorescence-activated cell sorting (FACS) in n=59 patients and results were compared with an age-matched healthy control group (n=45). DCs were stained (Fascin) in circular sections of the internal thoracic artery taken during bypass surgery and mature (CD83+) and immature (CD209+) DCs were differentiated. Cell densities were obtained digitally. Descriptive statistics and correlation analysis generated hypotheses.
Circulating myeloid DCPs were reduced (p<0.001), concomitant CKD added no further reduction. Immature DCs were predominantly detected in the adventitia, mature DCs dominated in the intima (p<0.001). In CKD, reduced circulating myeloid DCPs correlated with immature DCs in the vessel wall (r=0.87; p=0.002) and rather mature DCs were detectable; in patients without CKD immature DCs were dominating. C-reactive protein correlated with mature DCs (r=0.38; p=0.026).
ConclusionsQuantities of DCPs and mature and immature DCs vary between the vessel wall layers. The dominance of immature myeloid DCs in the adventitia suggests activation of the vascular-associated lymphoid tissue (VALT). Intensified inflammation associated with CKD may have promoted the maturation of myeloic DCs.
Related
- Urinary Bisphenol A Levels Are Associated with Vascular Injury in Mexican Patients with Coronary Artery Disease
- Berberine improves lipid levels, vascular function, and gut microbial-dependent metabolites in stable coronary artery disease patients
- Inflammation-based phenotyping and prognostic implications in patients with non-obstructive coronary artery disease.
- Predictive Value of the Systemic Immune-Inflammation Index for Cardiovascular Events in Patients With Chronic Total Coronary Occlusion
- Efficacy and Safety of Bioresorbable Vascular Scaffolds Versus Contemporary Drug-Eluting Stents in Coronary Artery Disease: A Systematic Review, Meta-Analysis, and Trial Sequential Analysis
BibTeX
@misc{ruhe2026dendritic,
title = {{Dendritic cells and vascular inflammation in patients with coronary artery disease with and without chronic kidney disease}},
author = {J. Ruhe and S. Böttger and T. Bondeva and I. Rohm and G. Färber and T. Doenst and D. Kretzschmar and G. Wolf and M. Busch},
howpublished = {medRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.01.26364549},
url = {https://www.medrxiv.org/content/10.64898/2026.10.01.26364549v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Dendritic cells and vascular inflammation in patients with coronary artery disease with and without chronic kidney disease
AU - J. Ruhe
AU - S. Böttger
AU - T. Bondeva
AU - I. Rohm
AU - G. Färber
AU - T. Doenst
AU - D. Kretzschmar
AU - G. Wolf
AU - M. Busch
PY - 2026
DA - 2026/10/01
PB - medRxiv
DO - 10.64898/2026.10.01.26364549
UR - https://www.medrxiv.org/content/10.64898/2026.10.01.26364549v1
AB - ObjectiveDendritic cells (DCs) are priming local immune responses, and their tissue-associated activity increases in inflammatory conditions. In patients with coronary artery disease (CAD), circulating dendritic cell precursors (DCPs) are reduced. This study investigates circulating DCPs and tissue densities of migrated DCs in CAD patients undergoing bypass surgery, focussing on patients with chronic kidney disease (CKD) as a risk factor for CAD and a condition of chronic vascular inflammation. Approach and ResultsCirculating DCPs were measured in the peripheral blood (before surgery) using fluorescence-activated cell sorting (FACS) in n=59 patients and results were compared with an age-matched healthy control group (n=45). DCs were stained (Fascin) in circular sections of the internal thoracic artery taken during bypass surgery and mature (CD83+) and immature (CD209+) DCs were differentiated. Cell densities were obtained digitally. Descriptive statistics and correlation analysis generated hypotheses. Circulating myeloid DCPs were reduced (p<0.001), concomitant CKD added no further reduction. Immature DCs were predominantly detected in the adventitia, mature DCs dominated in the intima (p<0.001). In CKD, reduced circulating myeloid DCPs correlated with immature DCs in the vessel wall (r=0.87; p=0.002) and rather mature DCs were detectable; in patients without CKD immature DCs were dominating. C-reactive protein correlated with mature DCs (r=0.38; p=0.026). ConclusionsQuantities of DCPs and mature and immature DCs vary between the vessel wall layers. The dominance of immature myeloid DCs in the adventitia suggests activation of the vascular-associated lymphoid tissue (VALT). Intensified inflammation associated with CKD may have promoted the maturation of myeloic DCs.
ER -