Ultra-fine Micronized Human Acellular Dermal Matrix Promotes Diabetic Wound Healing in a Mouse Model
4 affiliations
- 1College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea
- 2Department of R&D, Hulux, Seongnam, Republic of Korea
- 3Kyung Hee University Medical Center, Chamjoeun Hospital, Gwangju, Republic of Korea
- 4Department of R&D, Live Cell Instrument Co. Ltd, Namyangju, South Korea
Abstract
Background/Aim: Diabetic wounds are characterized by chronic inflammation, impaired angiogenesis, and abnormal extracellular matrix (ECM) remodeling, which contribute to delayed wound healing and non-healing ulcers. Because ECM degradation and impaired matrix repair are central features of diabetic wound pathology, we evaluated an ultra-fine micronized human acellular dermal matrix (UFM-hADM; mean particle size approximately 70 µm) as an injectable ECM scaffold for diabetic wound repair.
Materials and Methods: Public transcriptomic datasets from human diabetic foot ulcers were reanalyzed to assess ECM remodeling- and growth factor-related gene expression patterns and enriched wound repair-associated pathways. Full-thickness excisional wounds were then created on the dorsum of streptozotocin (STZ)-induced diabetic mice and treated with perilesional microinjections of UFM-hADM or saline. Wound closure was assessed by digital planimetry. Wound tissues were analyzed by histology, immunohistochemistry, and quantitative reverse transcription-polymerase chain reaction.
Results: Human diabetic foot ulcer datasets showed increased expression of several matrix metalloproteinase family members, altered ECM- and growth factor-related transcripts, and enrichment of tissue remodeling-associated pathways. In STZ-induced diabetic mice, UFM-hADM significantly improved wound closure compared with saline treatment, with near-complete epithelial coverage and more organized granulation tissue by Day 10. UFM-hADM-treated wounds showed increased expression of Col1a1, Col3a1, Tgfb1, and Egf, whereas Mmp1 and Mmp3 expressions were reduced. Immunohistochemistry showed increased CD31-positive microvessel density and α-smooth muscle actin-positive myofibroblast staining.
Conclusion: Human diabetic foot ulcer transcriptomic profiles support ECM remodeling and growth factor-related signaling as disease-associated features of chronic diabetic wounds. UFM-hADM improved wound repair in STZ-induced diabetic mice, with increased angiogenesis, reduced MMP expression, altered ECM-related gene expression, and more organized granulation tissue formation. These findings support further evaluation of UFM-hADM as an injectable human-derived ECM scaffold for chronic diabetic wounds.
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BibTeX
@article{hao2026ultrafine,
title = {{Ultra-fine Micronized Human Acellular Dermal Matrix Promotes Diabetic Wound Healing in a Mouse Model}},
author = {HAO JIN and BINNA SHIN and KEUN-WOO JANG and DASEUL KIM and SUBIN LEE and JIN YOUNG KIM and YONGJIN LEE and SANG-MYEONG LEE},
journal = {In Vivo},
year = {2026},
month = oct,
doi = {10.21873/invivo.14491},
url = {https://doi.org/10.21873/invivo.14491},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - Ultra-fine Micronized Human Acellular Dermal Matrix Promotes Diabetic Wound Healing in a Mouse Model
AU - HAO JIN
AU - BINNA SHIN
AU - KEUN-WOO JANG
AU - DASEUL KIM
AU - SUBIN LEE
AU - JIN YOUNG KIM
AU - YONGJIN LEE
AU - SANG-MYEONG LEE
PY - 2026
DA - 2026/10/29
JO - In Vivo
DO - 10.21873/invivo.14491
UR - https://doi.org/10.21873/invivo.14491
AB - Background/Aim: Diabetic wounds are characterized by chronic inflammation, impaired angiogenesis, and abnormal extracellular matrix (ECM) remodeling, which contribute to delayed wound healing and non-healing ulcers. Because ECM degradation and impaired matrix repair are central features of diabetic wound pathology, we evaluated an ultra-fine micronized human acellular dermal matrix (UFM-hADM; mean particle size approximately 70 µm) as an injectable ECM scaffold for diabetic wound repair. Materials and Methods: Public transcriptomic datasets from human diabetic foot ulcers were reanalyzed to assess ECM remodeling- and growth factor-related gene expression patterns and enriched wound repair-associated pathways. Full-thickness excisional wounds were then created on the dorsum of streptozotocin (STZ)-induced diabetic mice and treated with perilesional microinjections of UFM-hADM or saline. Wound closure was assessed by digital planimetry. Wound tissues were analyzed by histology, immunohistochemistry, and quantitative reverse transcription-polymerase chain reaction. Results: Human diabetic foot ulcer datasets showed increased expression of several matrix metalloproteinase family members, altered ECM- and growth factor-related transcripts, and enrichment of tissue remodeling-associated pathways. In STZ-induced diabetic mice, UFM-hADM significantly improved wound closure compared with saline treatment, with near-complete epithelial coverage and more organized granulation tissue by Day 10. UFM-hADM-treated wounds showed increased expression of Col1a1, Col3a1, Tgfb1, and Egf, whereas Mmp1 and Mmp3 expressions were reduced. Immunohistochemistry showed increased CD31-positive microvessel density and α-smooth muscle actin-positive myofibroblast staining. Conclusion: Human diabetic foot ulcer transcriptomic profiles support ECM remodeling and growth factor-related signaling as disease-associated features of chronic diabetic wounds. UFM-hADM improved wound repair in STZ-induced diabetic mice, with increased angiogenesis, reduced MMP expression, altered ECM-related gene expression, and more organized granulation tissue formation. These findings support further evaluation of UFM-hADM as an injectable human-derived ECM scaffold for chronic diabetic wounds.
SN - 0258-851X
SN - 1791-7549
ER -