Structural order emerges without mechanical order inside living cells
- 1Third institute of physics - Biophysics, University of Goettingen
Abstract
Intracellular organization is vital for cell function, and disruption of organelle positioning is connected to severe diseases, such as cancer. Given the small length scale of cells and the importance of Brownian motion, maintaining intracellular organization requires mechanical interactions to remain stable. Here, we test the common view that intracellular organization is reflected in intracellular mechanical properties. Using the recently introduced active mechanical fingerprint, we quantify intracellular mechanical properties and active forces to create mechanical maps of patterned RPE1 cells. The organization of actin, microtubules, the Golgi apparatus, the endoplasmic reticulum, and the nucleus reveals pronounced structural polarization across the cell population. To overcome limitations of simple averages, we use a Wasserstein barycenter to calculate an optimal intracellular organization that explains all measurements with minimal cellular distortion. To our surprise, and in contrast to previous reports, we find neither a statistically significant spatial mechanical pattern nor any relevant correlation between the tested structures and intracellular mechanical properties. While there is clear local mechanical anisotropy, the large heterogeneity of mechanical values outweighs any intracellular mechanical gradient. This is further supported by large cell-to-cell variability. Thus, despite having the same genome, cells function properly while their intracellular mechanical properties vary over two orders of magnitude.
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BibTeX
@misc{eskandari2026structural,
title = {{Structural order emerges without mechanical order inside living cells}},
author = {M. A. Eskandari and S. Ulmer and D. Carranza Zuniga and S. Lambert and S. Dey and C. Marcelli and H. Sarparandeh and R. Haag and D. Marx and M. Luber and B. E. Vos and B. Schmitzer and S. Koester and T. Betz},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.15.759452},
url = {https://www.biorxiv.org/content/10.64898/2026.10.15.759452v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Structural order emerges without mechanical order inside living cells
AU - M. A. Eskandari
AU - S. Ulmer
AU - D. Carranza Zuniga
AU - S. Lambert
AU - S. Dey
AU - C. Marcelli
AU - H. Sarparandeh
AU - R. Haag
AU - D. Marx
AU - M. Luber
AU - B. E. Vos
AU - B. Schmitzer
AU - S. Koester
AU - T. Betz
PY - 2026
DA - 2026/10/15
PB - bioRxiv
DO - 10.64898/2026.10.15.759452
UR - https://www.biorxiv.org/content/10.64898/2026.10.15.759452v1
AB - Intracellular organization is vital for cell function, and disruption of organelle positioning is connected to severe diseases, such as cancer. Given the small length scale of cells and the importance of Brownian motion, maintaining intracellular organization requires mechanical interactions to remain stable. Here, we test the common view that intracellular organization is reflected in intracellular mechanical properties. Using the recently introduced active mechanical fingerprint, we quantify intracellular mechanical properties and active forces to create mechanical maps of patterned RPE1 cells. The organization of actin, microtubules, the Golgi apparatus, the endoplasmic reticulum, and the nucleus reveals pronounced structural polarization across the cell population. To overcome limitations of simple averages, we use a Wasserstein barycenter to calculate an optimal intracellular organization that explains all measurements with minimal cellular distortion. To our surprise, and in contrast to previous reports, we find neither a statistically significant spatial mechanical pattern nor any relevant correlation between the tested structures and intracellular mechanical properties. While there is clear local mechanical anisotropy, the large heterogeneity of mechanical values outweighs any intracellular mechanical gradient. This is further supported by large cell-to-cell variability. Thus, despite having the same genome, cells function properly while their intracellular mechanical properties vary over two orders of magnitude.
ER -