PreprintCC BYbioRxiv
Fully Two-Photon-Driven RESOLFT Microscopy for Super-Resolution Imaging inside Tissue
- 1SANKEN, The University of Osaka
Abstract
Two-photon excitation (2PE) microscopy reaches deep into tissue but remains diffraction-limited, and existing 2PE-STED and 2PE-RESOLFT schemes still rely on single-photon depletion or photoswitching. Here we present 2P-RESOLFT microscopy, in which excitation and reversible photoswitching are both two-photon-driven. Using the positive photoswitchable protein Padron2, we achieved 205-nm lateral resolution and resolved previously unreported membrane protrusions of {beta} cells inside pancreatic pseudoislets.
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BibTeX
@misc{ozakinoma2026fully,
title = {{Fully Two-Photon-Driven RESOLFT Microscopy for Super-Resolution Imaging inside Tissue}},
author = {R. Ozaki-Noma and H. Ishii and T. Wazawa and J. Sakamoto and Y. Kozawa and Y. Kato and E. Mukai and T. Nemoto and T. Nagai},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.13.751305},
url = {https://www.biorxiv.org/content/10.64898/2026.09.13.751305v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Fully Two-Photon-Driven RESOLFT Microscopy for Super-Resolution Imaging inside Tissue
AU - R. Ozaki-Noma
AU - H. Ishii
AU - T. Wazawa
AU - J. Sakamoto
AU - Y. Kozawa
AU - Y. Kato
AU - E. Mukai
AU - T. Nemoto
AU - T. Nagai
PY - 2026
DA - 2026/09/13
PB - bioRxiv
DO - 10.64898/2026.09.13.751305
UR - https://www.biorxiv.org/content/10.64898/2026.09.13.751305v1
AB - Two-photon excitation (2PE) microscopy reaches deep into tissue but remains diffraction-limited, and existing 2PE-STED and 2PE-RESOLFT schemes still rely on single-photon depletion or photoswitching. Here we present 2P-RESOLFT microscopy, in which excitation and reversible photoswitching are both two-photon-driven. Using the positive photoswitchable protein Padron2, we achieved 205-nm lateral resolution and resolved previously unreported membrane protrusions of {beta} cells inside pancreatic pseudoislets.
ER -